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Preparation method for antibacterial peptide liposome

A technology of peptide lipids and antimicrobial peptides, applied in the field of biological preparations, can solve the problems of loss of activity and easy inactivation, and achieve the effects of easy acquisition, reduction of dosage, and improvement of bioavailability

Inactive Publication Date: 2012-05-23
TIANJIN RINGPU BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, after antimicrobial peptides enter the body, they are easily hydrolyzed by proteases in the digestive system to lose their activity, and they are also easily inactivated under natural conditions.

Method used

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  • Preparation method for antibacterial peptide liposome
  • Preparation method for antibacterial peptide liposome
  • Preparation method for antibacterial peptide liposome

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] The formula of liposome encapsulating active antimicrobial peptide is:

[0031] Component dosage

[0032] Phosphatidylcholine 9.87g

[0033] Cholesterol 2.221g

[0034] Vitamin E 0.1851g

[0035] Ethyl Paraben 0.0308g

[0036] Propylparaben 0.0308g

[0037] Ethanol 100mL

[0038] The formula of aqueous solution containing protective agent and active antimicrobial peptide is:

[0039] Component dosage

[0040] Antimicrobial peptide solid fermentation product drying powder 21.6g

[0041] Mannitol 5.0g

[0042] Sucrose 2.5g

[0043] Make up to 100mL with water for injection

[0044] The preparation method of the antimicrobial peptide liposome:

[0045] (1) Quantitatively weigh 9.87g of phosphatidylcholine, 2.221g of cholesterol, 0.1851g of vitamin E, 0.0308g of ethyl paraben and 0.0308g of propyl paraben and dissolve them in 100mL of ethanol, and ultrasonically disperse the solution evenly;

[0046] (2) Quantitatively weigh 21.6g of antimicrobial peptide solid fermentation product drying powd...

Embodiment 2

[0049] The formula of liposome encapsulating active antimicrobial peptide is:

[0050] Component dosage

[0051] Phosphatidylcholine 4.68g

[0052] Cholesterol 1.053g

[0053] Vitamin E 0.0878g

[0054] Ethyl Paraben 0.0293g

[0055] Ethanol 100mL

[0056] The formula of aqueous solution containing protective agent and active antimicrobial peptide is:

[0057] Component dosage

[0058] Antimicrobial peptide solid fermentation product drying powder 16.8g

[0059] Mannitol 6.0g

[0060] Sucrose 3.0g

[0061] Make up to 100mL with water for injection

[0062] The preparation method of the antimicrobial peptide liposome:

[0063] (1) Quantitatively weigh 4.68g of phosphatidylcholine, 1.053g of cholesterol, 0.0878g of vitamin E and 0.0293g of ethyl paraben in 100mL of ethanol, and ultrasonic treatment to make the solution uniformly dispersed and dissolved;

[0064] (2) Quantitatively weigh out 16.8g of antimicrobial peptide solid fermentation product drying powder, 6.0g of mannitol and 3.0g of sucrose...

Embodiment 3

[0067] The liposomes encapsulating active antimicrobial peptides are:

[0068] Component dosage

[0069] Cerephalin 4.53g

[0070] Octadecylamine 1.0193g

[0071] Vitamin E 0.0849g

[0072] Ethyl Paraben 0.0142g

[0073] Sorbic acid 0.0142g

[0074] Ethanol 100mL

[0075] The formula of aqueous solution containing protective agent and active antimicrobial peptide is:

[0076] Component dosage

[0077] Antimicrobial peptide solid fermentation product drying powder 15.2g

[0078] Gelatin 4.0g

[0079] Trehalose 2.0g

[0080] Make up to 100mL with water for injection

[0081] The preparation method of the antimicrobial peptide liposome:

[0082] (1) Quantitatively weigh 4.53g cephalin, 1.0193g octadecylamine, 0.0849g vitamin E, 0.0142g ethyl paraben, and 0.0142g sorbic acid and dissolve them in 100mL ethanol, and ultrasonically disperse and dissolve the solution;

[0083] (2) Quantitatively weigh 15.2g of antimicrobial peptide solid fermentation product drying powder, 4.0g gelatin and 2.0g trehalose,...

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Abstract

The invention provides a preparation method for an antibacterial peptide liposome. Medicinal active ingredients include an antibacterial peptide, a liposome film additive, a preservative and the like. The preparation method has the advantages of high embedding rate, stable preparation process, stable medicament effect, low leak rate and the like. The antibacterial peptide is prepared into the active antibacterial peptide liposome, so that the embedding rate of the antibacterial peptide can be increased, and the active period of the anti-bacteria peptide is prolonged.

Description

Technical field [0001] The invention belongs to the technical field of biological preparations, and particularly relates to a preparation method of active antimicrobial peptide liposomes. The method uses a liposome membrane as a shell to wrap the antimicrobial peptide to prolong the active protection period. Background technique [0002] Antibacterial peptides (ABP), also known as antimicrobial peptides (AMP) or peptide antibiotics, are widely distributed in bacteria, viruses, and various animals and plants, and have the ability to resist external microorganisms and eliminate mutant cells in the body. A class of small molecule peptides. Antibacterial peptides are a type of polypeptides that are encoded by specific genes of biological cells and induced by specific external conditions. As the first line of defense against external microorganisms, antibacterial peptides have powerful and extensive antibacterial functions. High antibacterial efficiency, broad antibacterial spectrum...

Claims

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Application Information

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IPC IPC(8): A61K9/127A61K38/02A61P31/04
Inventor 闫亮亮昌莉丽刘桂兰
Owner TIANJIN RINGPU BIO TECH
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