25 results about "Ethanol Injection" patented technology

The invention provides a preparation method of mitochondria-targeted antioxidant hybrid vesicles. The preparation method comprises the following steps: S1, preparing and separating adipose-derived stem cell nano-vesicles; s2, preparing a liposome loaded with dihydromyricetin and distearoyl phosphatidyl ethanolamine-polyethylene glycol 2000-triphenylphosphine targeting peptide by an ethanol injection method; and S3, preparing the hybrid nano vesicles loaded with the dihydromyricetin and the distearoyl phosphatidyl ethanolamine-polyethylene glycol 2000-triphenylphosphine targeting peptide. The invention also provides the hybrid vesicles prepared by the method and application of the hybrid vesicles in preparation of refractory diabetes wound treatment drugs. The lipidosome and the adipose-derived stem cell nano-vesicles are hybridized to prepare the hybridized vesicles capable of targeting mitochondria and resisting oxidation, the hybridized vesicles are applied to wound treatment for the first time, the problem of oxidative stress of diabetic wounds is solved, wound healing is accelerated, and a new strategy is provided for optimizing mitochondrial function defects and oxidative stress of the diabetic wounds.

The invention discloses a preparation method of a vitamin C liposome preparation capable of improving encapsulation efficiency, the vitamin C liposome preparation is prepared by an ethanol injection-high pressure microjet circulation homogenization method, the homogenized liposome is small in particle size and uniform in distribution, and meanwhile, by combining a dialysis purification and buffer agent pH regulation process, the encapsulation efficiency of the vitamin C liposome preparation is improved. The prepared vitamin C liposome preparation can still keep high stability under the condition that cholesterol is not contained, and the problem that in the prior art, a vitamin C liposome preparation is low in encapsulation efficiency is effectively solved.

The invention provides a lipidosome STING agonist delivery system based on a PD-L1 antibody as well as a preparation method and application of the lipidosome STING agonist delivery system, and belongs to the technical field of medical biology. Preparing lipidosome by adopting an ethanol injection method and an ammonium sulfate gradient technology; the PD-L1 antibody and the STING agonist can be loaded at the same time; the liposome is prepared by adopting an ethanol injection method and an ammonium sulfate gradient technology, and the STING agonist can be wrapped in the liposome in an active drug loading manner; dSPE-PEG2000-NHS and a PD-L1 antibody are mixed and incubated according to a specific proportion by utilizing a post-insertion method to form an antibody conjugated micelle, and the antibody conjugated micelle is fused with a blank liposome to realize antibody modification; secondly, the nano-liposome has good drug carrier characteristics and can effectively protect the loaded drug, reduce the risk of enzymolysis of the drug and improve the stability of the drug in vivo, so that the liposome drug delivery system simultaneously loading the nano-liposome and the nano-liposome is successfully prepared.

The invention discloses a nuclear magnetic signal separation method for water in a miscible solution in a porous medium, and relates to the technical field of fluid flow research, and the method comprises the following steps: S1, selecting a porous medium core sample to be detected, and obtaining the gas logging porosity and permeability of the core; s2, injecting ethanol into the saturated water rock core to simulate a displacement process, and obtaining relaxation time distribution of the rock core; s3, injecting ethanol into the dried rock core, and obtaining relaxation time distribution of the rock core; s4, heavy water is injected into the saturated water rock core, and relaxation time distribution of the rock core is obtained; s5, obtaining relaxation time distribution of water in a mixed solution of ethanol and water; s6, acquiring an ethanol injection PV critical value of a differential spectrum method; s7, obtaining an ethanol injection PV critical value of a time domain analysis method; and S8, selecting a differential spectrum method or a time-domain analysis method to separate a nuclear magnetic signal of water in a mixed solution of ethanol and water in the porous medium. The problem that the content and distribution change of water in the porous medium in the miscible-phase displacement process are difficult to accurately measure by an existing conventional displacement experiment method is solved.

The invention discloses a double-selenium lipid nanoparticle as well as a preparation method and application thereof. According to the nanoparticle, a skeleton is composed of cationic lipid, neutral phospholipid, cholesterol and functionalized PEG lipid, meanwhile, mRNA and a TLR agonist for coding therapeutic protein are carried, and the nanoparticle is synthesized through an ethanol injection method; the nanoparticle has both active targeting and strong transfection capabilities, the lipid nanoparticle is actively targeted to a tumor microenvironment and is taken by tumor cells by using biotin modified PEG lipid, and rapid'inclusion-lysosome 'escape is realized in the tumor cells by using trifluorogroup modified PEG lipid. Meanwhile, the nanoparticles are degraded in a glutathione / hydrogen peroxide dual-mode, so that the entrapped medicine is quickly and effectively released; the drug delivery system provided by the invention is simple in synthesis method, high in gene transfection efficiency and good in biological safety, and has potential value in anti-tumor research and clinical application.

The invention discloses a moisturizing anti-wrinkle polypeptide composition as well as a preparation method and application thereof, and relates to the technical field of daily chemical materials. The invention discloses a preparation method of a moisturizing and anti-wrinkle polypeptide composition, which comprises the following steps: uniformly stirring meadowfoam seed oil, caprylic acid / capric acid / succinic triglyceride and polypeptide liposome to obtain the moisturizing and anti-wrinkle polypeptide composition, wherein the polypeptide liposome is prepared by taking a combination of soybean lecithin, modified cholesterol and cholesterol as a capsule material and taking acetyl hexapeptide-8, snake venom peptide and a blue copper peptide compound as a core material through an ethanol injection method. The modified cholesterol is obtained by modifying cholesterol, the modified cholesterol is added into the capsule material, the defects that an existing liposome material is prone to aggregation and leakage, poor in stability and the like are effectively overcome, and the prepared material is good in stability, capable of being slowly released, high in skin retention amount and capable of being more easily absorbed by skin cells; the function of obviously delaying skin aging is realized.

The invention discloses a lipid nanoparticle-platelet conjugate as well as a preparation method and application thereof. The conjugate is formed by coupling lipid nanoparticles and platelets through a covalent modification method, a non-covalent modification method or a genetic engineering method, and the lipid nanoparticles can be loaded with nucleic acid, small molecules, protein / polypeptide drugs or CRISPR / Cas system components. The lipid nanoparticles have the drug carrying function of lipid nanoparticles and the natural spleen targeting characteristic of platelets, and the drug uptake rate and delivery efficiency of immune cells can be improved. The preparation method adopts manual mixing, ethanol injection or a microfluidic method, is simple, convenient and efficient, can be completed within several minutes, and does not damage platelets. According to the conjugate, the drug loading type and the coupling mode can be adjusted according to different diseases, the limitation of a single drug carrier is broken through, and the conjugate can adapt to various scenes such as tumor treatment and vaccine delivery and meet the personalized treatment requirement.

Described herein are light-activatable, sustained-exposure compositions for the treatment and monitoring of diseases along with methods of making and using the same, wherein the compositions comprise a solvent, a cellulosic polymer, a photosensitizer, and optionally additional therapeutic agents. In some embodiments, an additional therapeutic agent is included. The compositions can be administered to a target in need, such as a tumor or lesion, and activated with a light source to induce necrosis or apoptosis and to track the distribution of the composition at the target site. Beneficially the compositions provide synergistic ablation and photodynamic efficacy.

This application relates to the fields of chemistry and biomedicine, specifically to a tofenamic acid lipid composition, its preparation method, and its application. A tofenamic acid derivative is obtained by reacting a morpholine solution with tofenamic acid, ethyldimethylaminopropylcarbodiimide, and 4-dimethylaminopyridine, followed by purification. The morpholine in the morpholine solution is an N-alkylmorpholine compound. The tofenamic acid derivative is used to prepare tofenamic acid prodrug liposomes via ethanol injection, which are then used in combination with a STING agonist. The synergistic effect of the combined treatment stems from the dual regulation of the tumor immune microenvironment (TME), overcoming the immune escape induced by STING monotherapy. After STING agonist treatment, the intratumoral COX-2 / PGE2 axis is activated, leading to immunosuppression. The combination of tofenamic acid liposomes and a STING agonist inhibits COX-2, thus relieving this negative feedback loop.

This invention discloses a food-grade nanoliposome and its preparation method. Phosphatidylcholine is dissolved in anhydrous ethanol to obtain an organic phase. Ultrapure water or the water-soluble substance 5(6)-carboxyfluorescein is used as the aqueous phase. The organic and aqueous phases are mixed using a microfluidic method or an ethanol injection method to form a uniform lipid film, resulting in nanoliposomes applicable to the food industry. The aqueous phase is encapsulated within the hydrophilic region of the liposome. Since the liposomes prepared by this invention do not contain cholesterol or other substances besides phosphatidylcholine and have a good encapsulation rate, replacing the aqueous phase with nutrients allows for long-term consumption as food without causing excessive cholesterol levels in the human body. Furthermore, the encapsulation of nutrients by liposomes can improve the absorption of nutrients by the human body, demonstrating significant application potential in the food industry.

The invention relates to pearl eyesight-improving eye drops and a preparation method thereof.The pearl eyesight-improving eye drops are composed of pearl liquid, borneol, sodium glycerophosphate, poloxamer 188, ethyl alcohol and water for injection and can further contain sodium chloride and ethylparaben, the poloxamer 188, the sodium glycerophosphate and the ethyl alcohol serve as auxiliary materials of the pearl eyesight-improving eye drops, and the two auxiliary materials of the poloxamer 188 and the sodium glycerophosphate are used, so that the eyesight-improving effect of the pearl eyesight-improving eye drops is improved. According to the preparation method, the dosage of ethanol can be greatly reduced under the condition that borneol in the pearl eyesight-improving eye drops is completely dissolved, and in addition, the stability of the obtained pearl eyesight-improving eye drops is also remarkably improved.

The invention discloses a dendrobium officinale flavone nano-liposome with uric acid reducing activity as well as a preparation method and application thereof. The nano-liposome is prepared based on a sterol substance, a phospholipid substance and a cationic lipid. Wherein the sterol substance comprises phytosterol ester and cholesterol, the phospholipid substance is dipalmitoyl phosphatidylcholine (DPPC), and the cationic lipid is (2, 3-dioleoyl-propyl)-trimethylamine (DOTAP); and a functional active substance dendrobium officinale flavone is encapsulated. The dendrobium officinale flavone nano-liposome is prepared by adopting an ethanol injection method, and the nano-liposome can reduce the content of uric acid and xanthine oxidase, inhibit the level of inflammatory factors and relieve kidney and liver injury, so that hyperuricemia is relieved.

The present invention discloses an atomized inhalable fluorocarbon nanotransfection reagent for delivering nucleic acid drugs, and a preparation method and application thereof, and belongs to the field of gene transfection biotechnology. The present invention aims to solve the problem that nucleic acid system delivery is easily degraded by RNase enzymes in plasma and tissues, and the transfection efficiency needs to be improved. The nanotransfection reagent of the present invention includes cationic lipids, neutral phospholipids, cholesterol, auxiliary lipids, stabilizers, and fluorocarbon compounds; and is prepared by thin film dispersion method, ethanol injection method, reverse evaporation method, freeze drying method, freeze-thaw method, organic solvent volatilization method, and ultrasonic dispersion method. It is used for the encapsulation and delivery of nucleic acids. The particle size of the present invention is uniform, nanometer-scale, high in oxygen content, and can be atomized for inhalation administration. At the same time, the atomized nanotransfection reagent has the advantages of small toxic and side effects, good patient compliance, high transfection efficiency, and high encapsulation rate.

The application discloses a method for separating the nuclear magnetic signal of water in a miscible solution in a porous medium, and relates to the technical field of fluid flow research, and comprises the following steps: S1, selecting a porous medium core sample to be measured, and obtaining core gas measured porosity and permeability; S2, injecting ethanol into a water-saturated core to simulate a displacement process, and obtaining the relaxation time distribution of the core; S3, injecting ethanol into a dry core, and obtaining the relaxation time distribution of the core; S4, injecting heavy water into the water-saturated core, and obtaining the relaxation time distribution of the core; S5, obtaining the relaxation time distribution of water in a mixed solution of ethanol and water; S6, obtaining the ethanol injection PV critical value of a difference spectrum method; S7, obtaining the ethanol injection PV critical value of a time domain analysis method; and S8, selecting the difference spectrum method or the time domain analysis method to separate the nuclear magnetic signal of water in the mixed solution of ethanol and water in the porous medium, so that the problem that the content and distribution change of water in the porous medium in a miscible displacement process cannot be accurately measured by using a conventional displacement experiment method is solved.

The present invention discloses a method for preparing nucleic acid lipid nanoparticles targeting the liver and spleen by an ethanol injection method. Compared with the traditional microfluidics technology, the present invention adopts an ethanol injection method to add a lipid solution dropwise to complete the preparation of LNP under vortex mixing, thereby simplifying the operation process and reducing the equipment cost. The method of the present invention can fully mix lipids and nucleic acids to form nucleic acid lipid nanoparticles with good particle size and encapsulation rate, and has a good application prospect.

The application discloses a phospholipid-epicatechin liposome and a preparation method and application thereof, and relates to the field of food preservation technology. The preparation method of the phospholipid-epicatechin liposome comprises the following steps: taking a functional phospholipid fraction as a membrane material, taking epicatechin as an active component, and preparing the phospholipid-epicatechin liposome by an ethanol injection-ultrasonic method; and the functional phospholipid fraction is prepared by taking low-value sturgeon caviar as raw material, extracting by chloroform-methanol and purifying by silica gel column chromatography. The phospholipid-epicatechin liposome provided by the application can be applied to sturgeon caviar preservation, can effectively delay product brightness attenuation, inhibit abnormal accumulation of volatile basic nitrogen and malondialdehyde, retard excessive degradation of protein, and maximally retain key functional fatty acids such as EPA and DHA in the caviar.

The application discloses a curcumin-based ionic liquid liposome and a preparation method and application thereof. The preparation method of the curcumin-based ionic liquid liposome comprises the following steps: reacting curcumin with succinic anhydride to obtain curcumin succinate; reacting the curcumin succinate with betaine and L-carnitine to obtain a curcumin-based ionic liquid; and mixing the curcumin-based ionic liquid with lipids, and obtaining the curcumin-based ionic liquid liposome through an ethanol injection method. The curcumin-based ionic liquid liposome has good biocompatibility, improves the solubility and bioavailability of curcumin, so that the curcumin-based ionic liquid liposome can promote the penetration of the curcumin into the stratum corneum and has the effects of antioxidation, anti-inflammation and antitumor on the skin.

The invention belongs to the technical field of lavender liposome liquid band-aid, and discloses a lavender essential oil liposome liquid band-aid and a preparation method and application thereof, and the preparation method comprises the following steps: S1, raw material pretreatment; s2, extracting lavender essential oil; s3, the essential oil is placed in a centrifugal tube and subjected to refrigerated centrifugation, and upper-layer essential oil is taken; s4, preparing lavender lipidosome by adopting an ethanol injection method; and S5, adding 10% of polyvinyl alcohol, 10% of polyvinylpyrrolidone and glycerol into the lipidosome, grinding and uniformly mixing to obtain the liquid band-aid. The lavender essential oil is extracted through a supercritical CO2 fluid extraction method, the lipidosome with high encapsulation efficiency, high drug loading capacity and good stability is prepared through an ethanol injection method, and the lavender essential oil lipidosome liquid band-aid is prepared by adding a film forming material, a solvent and a plasticizer, so that the biological activity and stability of the lavender essential oil are improved, the lavender essential oil has slow release property, and the lavender essential oil lipidosome liquid band-aid can be used for preparing the lavender essential oil lipidosome liquid band-aid. The sensitization is reduced, and the effect of improving the drug effect is achieved.

The invention discloses an amomum villosum essential oil liposome with anti-inflammatory and anti-depression effects as well as a preparation method and application of the amomum villosum essential oil liposome. The amomum villosum essential oil liposome provided by the invention is prepared by injecting the lipid phase obtained by mixing the wall material, the emulsifier and the amomum villosum essential oil into the water phase through an ethanol injection method, the particle size distribution is uniform, the encapsulation efficiency is relatively high, and the stability and bioavailability of the amomum villosum essential oil are improved; meanwhile, the fructus amomi essential oil has better biological safety and no side effect, can be used for in-vivo drug-loading treatment, has a very good anti-inflammatory effect, can remarkably relieve the depressive symptom of a model mouse, further improves the in-vivo treatment effect of the fructus amomi essential oil, has better anti-inflammatory and anti-depression effects compared with original essential oil, and can be used for preparing a medicine. And more products and methods are provided for developing more anti-inflammatory or anti-depression drugs.

The application discloses an OCTN1-mitochondria double-targeting cascade delivery liposome of ergothioneine, a preparation method and application thereof, and belongs to the technical field of functional cosmetics and targeted drug preparations. The application adopts an ethanol injection method combined with a pH gradient active drug loading technology to prepare a core drug loading liposome, and sequentially modifies ceramide 3, an OCTN1 targeting ligand and a mitochondria targeting ligand on the surface, so as to construct a three-step cascade delivery system of efficient transdermal active cell uptake and mitochondria precise enrichment. The prepared liposome has an encapsulation efficiency of no less than 88%, a drug loading amount of no less than 9.8%, a 24h in-vitro transdermal cumulative permeation amount of no less than 15 mu g / cm 2 , an intracellular mitochondria enrichment rate of no less than 65%, and a mitochondria ROS clearance efficiency of no less than 87%, and the antioxidant and anti-photoaging effects of ergothioneine are significantly improved, so that the application can be widely applied to the fields of high-end anti-aging cosmetics, medical repair products and functional skin care products.

The invention belongs to the technical field of medicines, and particularly relates to preparation of Ag2Fe2O4-TiO2 (at) Rh2-PTX lipid nanoparticles and application of the Ag2Fe2O4-TiO2 (at) Rh2-PTX lipid nanoparticles to breast cancer resistance. The preparation method comprises the following steps: firstly, taking iron nitrate nonahydrate, silver nitrate and tetrabutyl titanate as raw materials, and preparing an Ag2Fe2O4-TiO2 magnetic nanoparticle core by adopting an ultrasonic chemical reduction method; and then coating egg yolk lecithin containing paclitaxel (PTX) and a cholesterol substitute ginsenoside solution on the surface of the inner core through an ethanol injection method to obtain the Ag2Fe2O4-TiO2 coated Rh2-PTX lipid nanoparticles. The nanoparticles prepared by the invention can combine near-infrared light (NIR) induced targeted photothermal therapy and immunotherapy to further play a synergistic anti-tumor treatment role, show excellent anti-breast cancer effect and biological safety, and provide a novel and efficient coping scheme for diagnosis and treatment integrated research of breast cancer.

The invention discloses a lipidosome gold nano composite material and a preparation method thereof. The preparation method comprises the following steps: preparing 25 nm gold particles by adopting a sodium citrate reduction method, and preparing a lipidosome with the diameter of about 142 nm and good monodispersity by adopting an ethanol injection method; a sample in which the volume ratio of a gold nano solution to a liposome solution is 1: 5 is selected for SERS detection; detailed analysis on the spectrum proves the existence of three main components, namely cholesterol, phosphatidylcholine and phosphatidyl ethanolamine, and finds that lipid molecules interact with gold nanoparticles through head groups; a strong vibration peak repeatedly appearing at 2120 cm <-1 > in a sample test is selected as a characteristic peak of a sample, the reliability and repeatability of liposome structure analysis by using the plasmon gold nanoparticles are further studied, a result shows that an SERS signal has good uniformity and repeatability, and the RSD of the local SERS signal is only 15.9%; on the basis of good uniformity and repeatability, the SERS hotspot map of the sample is also researched, and a prospect is provided for quantitative analysis.

The invention discloses a betacyanin nano-composite liposome, a preparation method and application of the betacyanin nano-composite liposome in preparation of an antioxidant, and belongs to the technical field of medicine and health care. According to the invention, betacyanin is embedded in liposome by using an ethanol injection method to prepare betacyanin liposome (Lip), and chitosan and sodium alginate are combined on the liposome through electrostatic interaction by using a layer-by-layer self-assembly technology. The preparation method comprises the following steps: preparing a chitosan-betacyanin composite liposome (Cs-Lip) and a sodium alginate-chitosan-betacyanin composite liposome (Sa-Cs-Lip); the DLS characterization of the composite liposome keeps good stability in the aspects of pH, storage, salt concentration, ion type and the like, the limitation that the betacyanin is easy to degrade is avoided, and particularly, the sodium alginate-chitosan-betacyanin composite liposome (Sa-Cs-Lip) also shows oxidation resistance superior to that of the betacyanin; therefore, the betacyanin has the potential to become a novel food additive for replacing betacyanin.

The present application relates to high sensitivity micro substance detection technical field, specifically is a kind of preparation PDMS flexible substrate gold particle transfer method, including the following steps: step one, preparation gold nanoparticles solution: step two, self-assembly into film: by liquid-liquid interface self-assembly method, with gold nanoparticles solution and n-hexane water oil interface is constructed, with ethanol injection into gold nanoparticles solution, water oil interface will gradually form a layer of nanometer gold particle film;Step three, preparation flexible SERS substrate.The present application can successfully transfer gold nanoparticles to flexible substrate;Gold particle film formed by liquid-liquid self-assembly is naturally settled on the surface of PDMS in keeping with the original close arrangement arrangement mode;The substrate prepared by the preparation method of the application has good detection effect on the analyte, and the limit can be measured 1×10 ‑8 mol / L with rhodamine 6G as raman active probe.

The invention relates to the technical field of pharmaceutical preparations, and discloses a preparation method and application of a novel dihydromyricetin derivatized butyric acid liposome, the liposome is composed of phospholipid, PEG lipid or butyrate thereof, cholesterol, dihydromyricetin or butyrate thereof; the components are dissolved in ethanol by using an ethanol injection method and then hydrated to form the liposome. As a delivery carrier, the liposome disclosed by the invention can deliver natural flavonoid compounds such as dihydromyricetin and the like, has the advantages of simplicity, easiness in preparation, low cost, good stability, low toxicity and wide application, and can play a synergistic treatment effect of dihydromyricetin and short-chain fatty acid butyric acid; in-vivo and in-vitro experiments show that the butyric acid derivatization dihydromyricetin lipidosome does not affect the original antioxidant effect of dihydromyricetin, the anti-inflammatory effect of a nano material can be remarkably improved, and the butyric acid derivatization dihydromyricetin lipidosome is suitable for treating chronic obstructive pulmonary diseases and other chronic inflammatory diseases and has synergistic interaction, low toxicity and industrial production potential.