The invention discloses verapamil liposome and a preparation method of the verapamil liposome, belonging to the technical field of medicine. The verapamil liposome is composed of verapamil, phospholipid, cholesterol, chitosan, cationic lipid and antioxidant, etc., all of which are in certain weight percentage. The verapamil liposome is prepared by adopting ammonium sulphate gradients method, film hydration method, ethanol injection method and reverse phase evaporation method. The invention adopts chitosan or derivatives of the chitosan to modify the surface of the liposome, and applies the chitosan to ophthalmic preparation, which can further increase corneal retention of the liposome and can remarkably improve the corneal penetration amount of the drug, with obvious advantages compared with ordinary liposome. With high entrapment efficiency, good stability, good biocompatibility, biological adhesiveness and biodegradability, the prepared liposome can realize sustained release and long-acting administration, particularly suitable for administration on ocular region, and can better play the ocular effects of anti-glaucoma and anti-cataract of verapamil.