13 results about "Ethanol Injection" patented technology

The invention provides a lipidosome STING agonist delivery system based on a PD-L1 antibody as well as a preparation method and application of the lipidosome STING agonist delivery system, and belongs to the technical field of medical biology. Preparing lipidosome by adopting an ethanol injection method and an ammonium sulfate gradient technology; the PD-L1 antibody and the STING agonist can be loaded at the same time; the liposome is prepared by adopting an ethanol injection method and an ammonium sulfate gradient technology, and the STING agonist can be wrapped in the liposome in an active drug loading manner; dSPE-PEG2000-NHS and a PD-L1 antibody are mixed and incubated according to a specific proportion by utilizing a post-insertion method to form an antibody conjugated micelle, and the antibody conjugated micelle is fused with a blank liposome to realize antibody modification; secondly, the nano-liposome has good drug carrier characteristics and can effectively protect the loaded drug, reduce the risk of enzymolysis of the drug and improve the stability of the drug in vivo, so that the liposome drug delivery system simultaneously loading the nano-liposome and the nano-liposome is successfully prepared.

The invention discloses a lipid nanoparticle-platelet conjugate as well as a preparation method and application thereof. The conjugate is formed by coupling lipid nanoparticles and platelets through a covalent modification method, a non-covalent modification method or a genetic engineering method, and the lipid nanoparticles can be loaded with nucleic acid, small molecules, protein / polypeptide drugs or CRISPR / Cas system components. The lipid nanoparticles have the drug carrying function of lipid nanoparticles and the natural spleen targeting characteristic of platelets, and the drug uptake rate and delivery efficiency of immune cells can be improved. The preparation method adopts manual mixing, ethanol injection or a microfluidic method, is simple, convenient and efficient, can be completed within several minutes, and does not damage platelets. According to the conjugate, the drug loading type and the coupling mode can be adjusted according to different diseases, the limitation of a single drug carrier is broken through, and the conjugate can adapt to various scenes such as tumor treatment and vaccine delivery and meet the personalized treatment requirement.

This application relates to the fields of chemistry and biomedicine, specifically to a tofenamic acid lipid composition, its preparation method, and its application. A tofenamic acid derivative is obtained by reacting a morpholine solution with tofenamic acid, ethyldimethylaminopropylcarbodiimide, and 4-dimethylaminopyridine, followed by purification. The morpholine in the morpholine solution is an N-alkylmorpholine compound. The tofenamic acid derivative is used to prepare tofenamic acid prodrug liposomes via ethanol injection, which are then used in combination with a STING agonist. The synergistic effect of the combined treatment stems from the dual regulation of the tumor immune microenvironment (TME), overcoming the immune escape induced by STING monotherapy. After STING agonist treatment, the intratumoral COX-2 / PGE2 axis is activated, leading to immunosuppression. The combination of tofenamic acid liposomes and a STING agonist inhibits COX-2, thus relieving this negative feedback loop.

This invention discloses a food-grade nanoliposome and its preparation method. Phosphatidylcholine is dissolved in anhydrous ethanol to obtain an organic phase. Ultrapure water or the water-soluble substance 5(6)-carboxyfluorescein is used as the aqueous phase. The organic and aqueous phases are mixed using a microfluidic method or an ethanol injection method to form a uniform lipid film, resulting in nanoliposomes applicable to the food industry. The aqueous phase is encapsulated within the hydrophilic region of the liposome. Since the liposomes prepared by this invention do not contain cholesterol or other substances besides phosphatidylcholine and have a good encapsulation rate, replacing the aqueous phase with nutrients allows for long-term consumption as food without causing excessive cholesterol levels in the human body. Furthermore, the encapsulation of nutrients by liposomes can improve the absorption of nutrients by the human body, demonstrating significant application potential in the food industry.

The application discloses a method for separating the nuclear magnetic signal of water in a miscible solution in a porous medium, and relates to the technical field of fluid flow research, and comprises the following steps: S1, selecting a porous medium core sample to be measured, and obtaining core gas measured porosity and permeability; S2, injecting ethanol into a water-saturated core to simulate a displacement process, and obtaining the relaxation time distribution of the core; S3, injecting ethanol into a dry core, and obtaining the relaxation time distribution of the core; S4, injecting heavy water into the water-saturated core, and obtaining the relaxation time distribution of the core; S5, obtaining the relaxation time distribution of water in a mixed solution of ethanol and water; S6, obtaining the ethanol injection PV critical value of a difference spectrum method; S7, obtaining the ethanol injection PV critical value of a time domain analysis method; and S8, selecting the difference spectrum method or the time domain analysis method to separate the nuclear magnetic signal of water in the mixed solution of ethanol and water in the porous medium, so that the problem that the content and distribution change of water in the porous medium in a miscible displacement process cannot be accurately measured by using a conventional displacement experiment method is solved.

The application discloses a phospholipid-epicatechin liposome and a preparation method and application thereof, and relates to the field of food preservation technology. The preparation method of the phospholipid-epicatechin liposome comprises the following steps: taking a functional phospholipid fraction as a membrane material, taking epicatechin as an active component, and preparing the phospholipid-epicatechin liposome by an ethanol injection-ultrasonic method; and the functional phospholipid fraction is prepared by taking low-value sturgeon caviar as raw material, extracting by chloroform-methanol and purifying by silica gel column chromatography. The phospholipid-epicatechin liposome provided by the application can be applied to sturgeon caviar preservation, can effectively delay product brightness attenuation, inhibit abnormal accumulation of volatile basic nitrogen and malondialdehyde, retard excessive degradation of protein, and maximally retain key functional fatty acids such as EPA and DHA in the caviar.

The application discloses a curcumin-based ionic liquid liposome and a preparation method and application thereof. The preparation method of the curcumin-based ionic liquid liposome comprises the following steps: reacting curcumin with succinic anhydride to obtain curcumin succinate; reacting the curcumin succinate with betaine and L-carnitine to obtain a curcumin-based ionic liquid; and mixing the curcumin-based ionic liquid with lipids, and obtaining the curcumin-based ionic liquid liposome through an ethanol injection method. The curcumin-based ionic liquid liposome has good biocompatibility, improves the solubility and bioavailability of curcumin, so that the curcumin-based ionic liquid liposome can promote the penetration of the curcumin into the stratum corneum and has the effects of antioxidation, anti-inflammation and antitumor on the skin.

The invention belongs to the technical field of lavender liposome liquid band-aid, and discloses a lavender essential oil liposome liquid band-aid and a preparation method and application thereof, and the preparation method comprises the following steps: S1, raw material pretreatment; s2, extracting lavender essential oil; s3, the essential oil is placed in a centrifugal tube and subjected to refrigerated centrifugation, and upper-layer essential oil is taken; s4, preparing lavender lipidosome by adopting an ethanol injection method; and S5, adding 10% of polyvinyl alcohol, 10% of polyvinylpyrrolidone and glycerol into the lipidosome, grinding and uniformly mixing to obtain the liquid band-aid. The lavender essential oil is extracted through a supercritical CO2 fluid extraction method, the lipidosome with high encapsulation efficiency, high drug loading capacity and good stability is prepared through an ethanol injection method, and the lavender essential oil lipidosome liquid band-aid is prepared by adding a film forming material, a solvent and a plasticizer, so that the biological activity and stability of the lavender essential oil are improved, the lavender essential oil has slow release property, and the lavender essential oil lipidosome liquid band-aid can be used for preparing the lavender essential oil lipidosome liquid band-aid. The sensitization is reduced, and the effect of improving the drug effect is achieved.

The application discloses an OCTN1-mitochondria double-targeting cascade delivery liposome of ergothioneine, a preparation method and application thereof, and belongs to the technical field of functional cosmetics and targeted drug preparations. The application adopts an ethanol injection method combined with a pH gradient active drug loading technology to prepare a core drug loading liposome, and sequentially modifies ceramide 3, an OCTN1 targeting ligand and a mitochondria targeting ligand on the surface, so as to construct a three-step cascade delivery system of efficient transdermal active cell uptake and mitochondria precise enrichment. The prepared liposome has an encapsulation efficiency of no less than 88%, a drug loading amount of no less than 9.8%, a 24h in-vitro transdermal cumulative permeation amount of no less than 15 mu g / cm 2 , an intracellular mitochondria enrichment rate of no less than 65%, and a mitochondria ROS clearance efficiency of no less than 87%, and the antioxidant and anti-photoaging effects of ergothioneine are significantly improved, so that the application can be widely applied to the fields of high-end anti-aging cosmetics, medical repair products and functional skin care products.

The invention discloses a lipidosome gold nano composite material and a preparation method thereof. The preparation method comprises the following steps: preparing 25 nm gold particles by adopting a sodium citrate reduction method, and preparing a lipidosome with the diameter of about 142 nm and good monodispersity by adopting an ethanol injection method; a sample in which the volume ratio of a gold nano solution to a liposome solution is 1: 5 is selected for SERS detection; detailed analysis on the spectrum proves the existence of three main components, namely cholesterol, phosphatidylcholine and phosphatidyl ethanolamine, and finds that lipid molecules interact with gold nanoparticles through head groups; a strong vibration peak repeatedly appearing at 2120 cm <-1 > in a sample test is selected as a characteristic peak of a sample, the reliability and repeatability of liposome structure analysis by using the plasmon gold nanoparticles are further studied, a result shows that an SERS signal has good uniformity and repeatability, and the RSD of the local SERS signal is only 15.9%; on the basis of good uniformity and repeatability, the SERS hotspot map of the sample is also researched, and a prospect is provided for quantitative analysis.

The invention discloses a betacyanin nano-composite liposome, a preparation method and application of the betacyanin nano-composite liposome in preparation of an antioxidant, and belongs to the technical field of medicine and health care. According to the invention, betacyanin is embedded in liposome by using an ethanol injection method to prepare betacyanin liposome (Lip), and chitosan and sodium alginate are combined on the liposome through electrostatic interaction by using a layer-by-layer self-assembly technology. The preparation method comprises the following steps: preparing a chitosan-betacyanin composite liposome (Cs-Lip) and a sodium alginate-chitosan-betacyanin composite liposome (Sa-Cs-Lip); the DLS characterization of the composite liposome keeps good stability in the aspects of pH, storage, salt concentration, ion type and the like, the limitation that the betacyanin is easy to degrade is avoided, and particularly, the sodium alginate-chitosan-betacyanin composite liposome (Sa-Cs-Lip) also shows oxidation resistance superior to that of the betacyanin; therefore, the betacyanin has the potential to become a novel food additive for replacing betacyanin.

The present application relates to high sensitivity micro substance detection technical field, specifically is a kind of preparation PDMS flexible substrate gold particle transfer method, including the following steps: step one, preparation gold nanoparticles solution: step two, self-assembly into film: by liquid-liquid interface self-assembly method, with gold nanoparticles solution and n-hexane water oil interface is constructed, with ethanol injection into gold nanoparticles solution, water oil interface will gradually form a layer of nanometer gold particle film;Step three, preparation flexible SERS substrate.The present application can successfully transfer gold nanoparticles to flexible substrate;Gold particle film formed by liquid-liquid self-assembly is naturally settled on the surface of PDMS in keeping with the original close arrangement arrangement mode;The substrate prepared by the preparation method of the application has good detection effect on the analyte, and the limit can be measured 1×10 ‑8 mol / L with rhodamine 6G as raman active probe.

The invention relates to the technical field of pharmaceutical preparations, and discloses a preparation method and application of a novel dihydromyricetin derivatized butyric acid liposome, the liposome is composed of phospholipid, PEG lipid or butyrate thereof, cholesterol, dihydromyricetin or butyrate thereof; the components are dissolved in ethanol by using an ethanol injection method and then hydrated to form the liposome. As a delivery carrier, the liposome disclosed by the invention can deliver natural flavonoid compounds such as dihydromyricetin and the like, has the advantages of simplicity, easiness in preparation, low cost, good stability, low toxicity and wide application, and can play a synergistic treatment effect of dihydromyricetin and short-chain fatty acid butyric acid; in-vivo and in-vitro experiments show that the butyric acid derivatization dihydromyricetin lipidosome does not affect the original antioxidant effect of dihydromyricetin, the anti-inflammatory effect of a nano material can be remarkably improved, and the butyric acid derivatization dihydromyricetin lipidosome is suitable for treating chronic obstructive pulmonary diseases and other chronic inflammatory diseases and has synergistic interaction, low toxicity and industrial production potential.