Cordyceps sinensis polysaccharide liposome medicament and preparation thereof

A technology of Cordyceps polysaccharides and liposomes, applied in liposome delivery, drug combination, antineoplastic drugs, etc., can solve the problems of inability to achieve high bioavailability, short half-life, short maintenance time of effective blood drug concentration, etc. Achieve the effect of maintaining effective blood drug concentration and good absorption and utilization

Inactive Publication Date: 2009-04-08
JILIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the absorption and digestion of Cordyceps polysaccharides in the body is too fast, the half-life is short, and high bioavailability cannot be achieved, and

Method used

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  • Cordyceps sinensis polysaccharide liposome medicament and preparation thereof
  • Cordyceps sinensis polysaccharide liposome medicament and preparation thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0044] Preparation of Cordyceps polysaccharide liposomes by reverse evaporation method: Weigh 100 mg of soybean lecithin, 100 mg of cholesterol, and 200 mg of Cordyceps polysaccharide, dissolve them in 10 ml of chloroform, add 10 ml of phosphate buffer (0.01mol / L), and then ultrasonicate in a water bath for 15 minutes to form a uniform single liposome. For the phase system, evaporate under reduced pressure (60°C) to remove chloroform to gel, then add 15ml of phosphate buffer (0.01mol / L), and continue to evaporate under reduced pressure (60°C) to form an aqueous suspension, that is, liposome formation. The encapsulation rate can reach 62.3%, and the burst release rate is 4.41%.

Embodiment 2

[0046] Preparation of Cordyceps polysaccharide liposomes by reverse evaporation method: Weigh 400 mg of soybean lecithin, 200 mg of cholesterol, and 200 mg of Cordyceps polysaccharide, dissolve them in 15 ml of chloroform, add 20 ml of phosphate buffer (0.01 mol / L), and then ultrasonicate in a water bath for 15 min to form a uniform single liposome. For the phase system, evaporate under reduced pressure (60°C) to remove chloroform to gel, then add 15ml of phosphate buffer (0.01mol / L), and continue to evaporate under reduced pressure (60°C) to form an aqueous suspension, that is, liposome formation. The encapsulation rate can reach 59.4%, and the burst release rate is 4.98%.

Embodiment 3

[0048] Preparation of Cordyceps polysaccharide liposomes by thin-film ultrasonic method: Weigh 150 mg of soybean lecithin, 50 mg of cholesterol, and 200 mg of Cordyceps polysaccharide, dissolve in 10 ml of chloroform, evaporate under reduced pressure (50 ° C) to form a film and remove the organic solvent, then add 25 ml of phosphate buffer (0.01mol / L) to wash the membrane, and then ultrasonically in a water bath for 20min, pass through a 0.8μm microporous membrane to complete the particle, that is, liposomes are formed. The encapsulation rate can reach 47.4%, and the burst release rate is 5.12%.

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Abstract

The invention relates to liposome of extract of cordyceps and a method for preparing the same. The main composition of the extract is polysaccharide. The liposome of the cordyceps is prepared by using phospholipid and cholesterol as membrane materials, and the prepared liposome of the cordyceps can be further prepared into dosage forms of tablet, injection, oral agent, capsule, granule, freeze-dried powder and the like. The method for preparing the liposome of the cordyceps can adopt a reverse evaporation method, a film ultrasound method and an ethanol injection method, the encapsulation rate of the methods can reach more than 40 percent, and the in vitro burst rate is low. The preparing process improves the stability of the drug. After the polysaccharide of the cordyceps is coated with the liposome, the polysaccharide of the cordyceps can be absorbed and used better in human body; and through the slow release effect of the liposome, the effective blood drug level of the drug can be maintained for a longer time.

Description

technical field [0001] The invention discloses a cordyceps mycelium polysaccharide liposome drug, and also discloses a preparation process of the liposome, belonging to the technical field of extraction and application of active ingredients of traditional Chinese medicines. Background technique [0002] Cordyceps is a complex of sub-seats and larval corpses that parasitize on the larvae of the larval moths. It is a traditional precious and nourishing Chinese herbal medicine, and it is also known as the three major Chinese tonics together with ginseng and deer antler. It has effects on regulating the immune system of the body, resisting tumors, improving the heart's ability to withstand hypoxia, reducing blood cholesterol and triglycerides, and fighting fatigue. [0003] At present, people have researched and analyzed the composition and pharmacological effects of Cordyceps sinensis. There are many active ingredients such as cordycepic acid, cordycepin, adenosine, amino acid...

Claims

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Application Information

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IPC IPC(8): A61K9/127A61K31/715A61K36/068A61P37/02A61P35/00A61P9/10A61P3/06
Inventor 滕利荣孟庆繁逯家辉郑丹谢晶姜丹王迪金璐
Owner JILIN UNIV
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