Breast cancer treatment triptolide liposome preparation and preparation method thereof

A technology of triptolide and liposome preparation, which is applied in the field of traditional Chinese medicine, can solve the problems of large toxic and side effects, unsatisfactory curative effect, and low curative effect of cancer, and achieve the effect of growth inhibition

Inactive Publication Date: 2013-12-25
NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Chemotherapeutic drugs have a certain eff...

Method used

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  • Breast cancer treatment triptolide liposome preparation and preparation method thereof
  • Breast cancer treatment triptolide liposome preparation and preparation method thereof
  • Breast cancer treatment triptolide liposome preparation and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0026] Embodiment 1: A kind of triptolide liposome preparation for the treatment of breast cancer is made of triptolide, and the preparation method of its liposome is as follows:

[0027] (1) The ratio of raw materials for preparing liposomes is: lecithin 900mg, amphipathic polyethylene glycol 2000-distearoylphosphatidylethanolamine 90mg, cholesterol 90mg;

[0028] (2) The preparation method of triptolide liposomes is prepared by ethanol injection method: take 90ml of PBS buffer solution with pH 7.4 in an eggplant-shaped bottle, and preheat it in a constant temperature water bath at 50°C for 35min;

[0029] (3) Take the liposome proportioned in step 1 in a beaker, add 36 mg of triptolide, and then add an appropriate amount of absolute ethanol to dissolve it;

[0030] (4) After the triptolide is completely dissolved, quickly inject it into the eggplant-shaped bottle with a buffer solution preheated at a constant temperature in step 2 to obtain a milky white liposome suspension,...

Embodiment 2

[0031] Embodiment 2: A kind of triptolide liposome preparation for the treatment of breast cancer is made of triptolide, and the preparation method of its liposome is as follows:

[0032] (1) The ratio of raw materials for preparing liposomes is: lecithin 600mg, amphiphilic polyethylene glycol 2000-distearoylphosphatidylethanolamine 60mg, cholesterol 60mg;

[0033] (2) The preparation method of triptolide liposomes is prepared by ethanol injection method: take 90ml of PBS buffer solution with pH 7.4 in an eggplant-shaped bottle, and preheat it in a constant temperature water bath at 55°C for 30min;

[0034] (3) Take the liposome proportioned in step 1 in a beaker, add 24mg of triptolide, and then add an appropriate amount of absolute ethanol to dissolve it;

[0035] (4) After triptolide is completely dissolved, quickly inject it into the eggplant-shaped bottle with buffer solution preheated at constant temperature in step 2 to obtain a milky white liposome suspension, continue...

Embodiment 3

[0036] Example 3: Inhibition of a triptolide liposome preparation on transplanted breast cancer in nude mice

[0037] 1. Experimental animals and tumor strains

[0038] 50 female BALB / C-nu / nu nude mice, weighing 18-20g, were purchased from the Animal Experiment Center of our school. Human breast cancer cell line MCF-7 was purchased from the Animal Experiment Center of Jiangsu Cancer Institute.

[0039] 2. Modeling and experimental methods

[0040] Of 50 BALB / C-nu / nu nude mice, 10 were randomly reserved in proportion as the negative control group, and the remaining 40 were used for modeling. Take human breast cancer MCF-7 cells in the logarithmic growth phase cultured in vitro, and adjust the cell concentration to 1×10 7 / ml. Under sterile conditions, human breast cancer MCF-7 cells were inoculated on the back of BALB / C-nu / nu nude mice with an inoculation volume of 0.2ml / mouse (the number of cells was 2×10 6 / Only). The entire inoculation process must be carried out in a ...

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Abstract

The present invention discloses a breast cancer treatment triptolide liposome preparation and a preparation method thereof, wherein the breast cancer treatment triptolide liposome preparation is a liposome preparation prepared from a breast cancer treatment traditional Chinese medicine active effective component triptolide. The preparation method comprises: adopting an ethanol injection method to prepare liposomes to obtain the breast cancer (breast cancer and triple-negative breast cancer) treatment triptolide liposome preparation. According to the breast cancer treatment triptolide liposome preparation, in vivo animal anti-cancer pharmacological experiment results show that: significant growth inhibition effects are provided for breast cancer and triple-negative breast cancer, and anti-cancer activity of the breast cancer treatment triptolide liposome preparation is respectively and significantly higher than anti-cancer activities of chemotherapy drugs such as paclitaxel and cisplatin. In addition, the triptolide liposome preparation provides a higher anti-cancer effect than the triptolide non-liposome preparation.

Description

1. Technical field [0001] The invention relates to the technical field of traditional Chinese medicine, in particular to a triptolide liposome preparation for treating breast cancer and a preparation method thereof. 2. Background technology [0002] Cancer is a formidable enemy that endangers human health. There are about 1.6 million new cancer cases in my country every year, and about 1.3 million people die from cancer every year. The number of deaths due to cancer in the country is increasing year by year. my country is a high-incidence area for breast cancer, lung cancer and other cancers, and the cancer mortality rate is high. No more. [0003] There are currently no effective drugs that cure most cancer patients. Although chemotherapeutic drugs have certain curative effects on cancer, they have severe side effects, and Chinese patent medicines generally have low curative effects on cancer, and the curative effects are not ideal. [0004] The present inventor has studi...

Claims

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Application Information

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IPC IPC(8): A61K9/127A61K31/585A61P35/00
Inventor 章永红章迅
Owner NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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