Preparation method of galactosylated chitosan modified immune ethosome

Abstract

The invention relates to a preparation method of a galactosylated chitosan modified immune ethosome. The preparation method comprises the following steps: mixing lecithin, cholesterol and octadecylamine, then dissolving in ethanol, carrying out rotary evaporation to obtain a liposome membrane, then adding antigen protein solution, and carrying out ultrasonic dispersion to obtain an immune ethosome loaded with antigen protein; by utilizing a layer-by-layer self-assembly technology, dropwise adding the immune ethosome loaded with the antigen protein into hyaluronic acid (HA) solution, stirring to obtain mixed solution, then dropwise adding into galactosylated chitosan (GC) solution, and stirring, so that the galactosylated chitosan modified immune ethosome is obtained. The prepared GC modified immune ethosome has the advantages of high deformability, high encapsulation efficiency, cutin softening and complete skin permeation (so that an organism is immunized and obtains corresponding resistance), no stimulation to skin, stable properties and simple preparation method and is an ideal carrier for constructing a transcutaneous immune patch.

Description

technical field [0001] The invention belongs to the field of preparation of immune ethosomes, in particular to a method for preparing immune ethosomes modified by galactosylated chitosan. Background technique [0002] Transcutaneous immunization (TCI) is a new type of immunization strategy developed in recent years. It is a new method for inducing a systemic immune response by applying antigens and adjuvants locally to the skin. The defects such as low immune efficiency and easy to produce tolerance have broad application prospects. At present, the main obstacle limiting its application is the barrier effect of the stratum corneum on the skin surface, which makes it difficult for the macromolecular antigenic substances of the vaccine to pass through the skin surface and enter the active epidermis and dermis rich in antigen-presenting cells (Antigen-presenting cells, APCs). Therefore, studying a novel transcutaneous immune system without sacrificing skin integrity is the key...

AI Technical Summary

Problems solved by technology

[0002] Transcutaneous immunization (TCI) is a new type of immunization strategy developed in recent years. It is a new method for inducing a systemic immune response by applying antigens and adjuvants locally to the skin. Low immune efficiency, prone to tolerance, etc., have broad application prospects

At present, the main obstacle limiting its application is the barrier effect of the stratum corneum on the skin surface, which makes it difficult for the macromolecular antigenic substances of the vaccine to pass through the skin surface and enter the active epidermis and dermis rich in antigen-presenting cells (Antigen-presenting cells, APCs). Therefore, studying a novel transcutaneous immune system without sacrificing skin integrity is the key to developing transcutaneous immunity

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