Thermosensitive liposome as well as preparation method and application thereof

A heat-sensitive liposome and liposome preparation technology, applied in the field of liposome embedding, can solve problems that have not been reported

Inactive Publication Date: 2015-07-22
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the current reports, the core materials embedded in thermosensitive liposomes are mainly drugs with relatively large molecular weights, and there is no report on the embedding of flavor substances with small molecular weights.

Method used

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  • Thermosensitive liposome as well as preparation method and application thereof
  • Thermosensitive liposome as well as preparation method and application thereof
  • Thermosensitive liposome as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Thermosensitive liposomes embedded with β-ionone (phosphatidylcholine and hydrogenated lecithin molar ratio 4:1)

[0034] Materials and dosage:

[0035] Composite phospholipid material 200mg; long circulation material 30mg; Tween80 100mg; β-ionone 25mg.

[0036] Preparation:

[0037] Weigh various materials according to the above dosage into a round bottom flask (250ml), add absolute ethanol in a water bath at 50°C until dissolved, remove the ethanol by rotary evaporation under reduced pressure, and form a thin film. PBS buffer solution was added, hydrated by rotary evaporation (50°C), cooled rapidly, and ultrasonically treated in an ice bath at 360W on a probe-type ultrasonic instrument for 4 minutes. After dissolving in water at 50°C for 2 hours, let it stand overnight at 4°C, remove titanium particles by refrigerated centrifugation, and fill with N 2 After sealing, the thermosensitive liposome embedding β-ionone is obtained. After adding the protective agent, the...

Embodiment 2

[0040] Thermosensitive liposomes embedded with β-ionone (molar ratio of phosphatidylcholine to hydrogenated lecithin is 3:2)

[0041] Take 200mg of composite phospholipid material; 100mg of Tween80; 30mg of long-circulation material and 50mg of β-ionone. The preparation process of liposome is identical with example 1.

[0042] The obtained liposome has a particle diameter of about 170 nm and an encapsulation efficiency of about 35% as measured by a nanometer particle size distribution analyzer. DSC measurement results show that its phase transition temperature is around 43°C. The liposome can keep stable for at least 1 month at 25°C, and the dried powder liposome can keep stable for at least 3 months at 25°C. Experiments show that at 37°C, β-ionone is released less, and from 42°C, it is released rapidly.

Embodiment 3

[0044] Thermosensitive Liposomes Encapsulating Rose Essential Oil

[0045] Take 200mg of compound phospholipid material; 100mg of Tween80; 30mg of long circulation material and 25mg of rose essential oil. The preparation process of liposome is identical with example 1.

[0046] The obtained liposome has a particle diameter of about 200 nm and an encapsulation efficiency of about 45% as measured by a nanometer particle size distribution analyzer. The phase transition temperature shown by the DSC spectrum is around 41°C. Stability results showed that the temperature-sensitive liposome remained stable at 25°C for at least 1 month, and remained stable at 25°C for at least 3 months after powdering. Experiments have shown that rose essential oil releases less at 37°C, and it releases rapidly from 42°C.

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Abstract

The invention discloses thermosensitive liposome as well as a preparation method and application thereof. The thermosensitive liposome uses phospholipid as a membrane material of the thermosensitive liposome, and is prepared by adding a membrane structure regulating agent and a phase change regulating agent in the preparation process, wherein the phospholipid comprises dipalmitoyl phosphatidyl choline and hydrogenated soybean phospholipid in a mol ratio ranging from 6:4 to 10:0; the phase change regulating temperature range is 35-50 DEG C; the mass ratio of the phospholipid to the membrane structure regulating agent to the phase change regulating agent is 20-40: 0-10: 5-20. The liposome is applied to a cigarette filter, can keep stable at room temperature, and can quickly release embedded substances in the cigarette smoking process.

Description

technical field [0001] The invention relates to the technical field of liposome embedding, in particular to a thermosensitive liposome preparation and its application in cigarettes. Background technique [0002] Embedding preparations containing flavor substances are widely used in cigarettes, and these preparations can be added to shredded tobacco, cigarette paper, filter tip and tipping paper. Among them, the embedding technology added to the filter has unique advantages: it avoids the loss of the core material during the storage of the cigarette and the pyrolysis during the burning and smoking process; it avoids the risk of increasing the harmfulness of the cigarette due to the burning of the wall material of the embedding agent. The embedding agent added to the filter tip can break the capsule shell with the help of changes in external conditions, such as: extrusion of fingers or lips, changes in temperature, changes in pH or air pressure, etc. Tobacco companies at home...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01J13/02C11B9/00A24D3/14
CPCB01J13/02A24D3/14C11B9/00
Inventor 钟芳周志磊梁蓉李玥徐菲菲王一涵陈羚
Owner JIANGNAN UNIV
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