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Method for embedding biological specimen with high water content

A technology of biological specimens and water content, applied in the direction of measuring devices, test sample preparation, instruments, etc., can solve the problems of unusable embedding and achieve good embedding effect

Inactive Publication Date: 2008-04-16
CHONGQING NORMAL UNIVERSITY
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  • Summary
  • Abstract
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  • Claims
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AI Technical Summary

Problems solved by technology

[0002] At present, the use of transparent urea-formaldehyde resin to embed biological specimens is mainly limited to small insects and small plants. For biological specimens with a large water content, they cannot be used for embedding without special treatment.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0013] Embedding of jellyfish specimens

[0014] (1) Fix, sterilize, and preserve the jellyfish; cleaning: take out the specimen immersed in the preservative, and repeatedly rinse with clean water to remove the preservative; plasticization: use polyethylene with a molecular weight of 600 from low to high. Glycol is used as a plasticizer to plasticize the specimen so that polyethylene glycol can completely replace the moisture in the specimen;

[0015] (2) Take out the specimen plasticized by polyethylene glycol and put it into a porcelain plate with absorbent paper, transfer it to a 30-40°C incubator and place it for 12-24 hours to complete the treatment of the specimen before embedding ;

[0016] (3) Take the prepared transparent urea-formaldehyde resin and fully fuse with the plasticized biological specimen: add transparent urea-formaldehyde resin as a primer, put the specimen obtained in step (2) after it solidifies, and insert the specimen with an insect needle or stainle...

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Abstract

The invention relates to an encapsulating method for a biological specimen. The technical proposal adopted is that: a biological specimen is fixed, sterilized, antisepticised and cleaned, polyoxyethylene (PEG six hundred) is used to plasticize the specimen, the plasticized specimen is placed in a porcelain dish which is provided with absorbent paper, is then put into an incubator of thirty degrees to forty degrees for twelve to twenty-four hours and are finally fully integrated with a prepared transparent Lauxite until the preparation of specimen is completed. The encapsulating method has the advantages of a good encapsulating effect, mould proofing, insect prevention and color retention, which is particularly applicable to encapsulating the biological specimen with more water content, such as Craspedacusta, earthworms, mollusks and small-sized fishes.

Description

technical field [0001] The invention relates to an embedding method of biological specimens, in particular to an embedding method of biological specimens with relatively high water content. Background technique [0002] Currently available techniques for embedding biological specimens with transparent urea-formaldehyde resin are mainly limited to small insects and small plants. For biological specimens with high water content in the body, they cannot be used for embedding without special treatment. Contents of the invention [0003] Aiming at the deficiencies of the existing embedding technology, the present invention provides a method for embedding biological specimens with high water content, and the specific technical scheme is as follows: [0004] A kind of embedding method to the biological sample with more water content, it is characterized in that following steps are carried out: [0005] (1) Fix, sterilize, preserve, clean, and plasticize the collected biological ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/28G01N33/483
Inventor 唐安科唐发辉赵元莙
Owner CHONGQING NORMAL UNIVERSITY
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