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Influenza A,B virus antigen colloidal gold combined detection test paper

A technology for detecting test strips and test strips, applied in the field of B virus antigen detection test strips and influenza A, can solve the problems of long time, complicated operation, cumbersome and other problems, and achieve the effects of easy promotion, clear and easy to distinguish results, and simple operation.

Inactive Publication Date: 2007-01-31
BEIJING ZHUANGDI HAOHE BIOMEDICINE SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It overcomes the shortcomings of the existing technology, such as complex operation, cumbersome operation, long time, need for instruments, and inability to detect two antigens at the same time

Method used

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  • Influenza A,B virus antigen colloidal gold combined detection test paper
  • Influenza A,B virus antigen colloidal gold combined detection test paper
  • Influenza A,B virus antigen colloidal gold combined detection test paper

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Embodiment 1: Influenza A, B virus antigen joint detection test strip (referring to Fig. 1)

[0025] The reaction support is 6.2cm×0.4cm PCV plate; the absorbent pad is 2cm×0.4cm oil filter paper; the nitrocellulose membrane of 1.8cm×0.4cm is coated with anti-mouse IgG, FluA-McAb, FluB-McAb in sequence; ×0.4cm colloidal gold-labeled FluA, B-McAb glass fiber membrane; the gold-labeled antibody protective film is a 2.7cm×0.4cm polyester film; that is, the joint detection test strip for influenza A and B virus antigens is formed.

Embodiment 2

[0026] Embodiment 2: detection method (see figure 2 )

[0027] Take nasopharyngeal secretions, put them into a vial containing cell lysate, insert the test strip "4" of Example 1 into the bottle (the test strip can not be taken out until the result is observed), the liquid in the sample Suction upward, 10-15 minutes to interpret the results:

[0028] If it contains influenza A or B antigen, it will form a corresponding complex with the colloidal gold-labeled influenza A or B-McAb on the test strip, and combine with the influenza A or B-McAb coated on the nitrocellulose membrane to form a red color. Lines, i.e. forming red bands at "A" or "B".

[0029] Regardless of whether it contains the corresponding antigen or not, the colloidal gold-labeled McAb continues to crawl upward and forms a red precipitation line with the anti-mouse IgG coated on the membrane, that is, a red band is formed at "C". This line is a quality control line. If the colloidal gold fails, this line will ...

experiment example 1

[0031] Experimental Example 1: Clinical Test Results

[0032] After testing 1005 samples of influenza-like patients, influenza A and B viruses were simultaneously infected, and three bands A, B, and C appeared in 1 case, accounting for 0.01%; Influenza A virus infection, two bands A and C appeared in 374 cases, accounting for 37.2% ; There were 413 cases of influenza B virus infection with two bands B and C, accounting for 41.1%. Compared with the conventionally used indirect fluorescence method at present, the sensitivity is 99.8%, and the specificity is 100%.

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PUM

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Abstract

The invention supplies a rapid testing tape that could simultaneously test A, B virus antigen. It covers FluA-McAb, FluB-McAb and IgG on NC film, compounds FluA-McAb, FluB-McAb marked by colloidal gold, simultaneously tests A, B virus antigen in tested sample by using film chromatography double antibody cream filling method. The invention could decrease cockamamie process, and could gain result in 10-15 minutes. It is suitable to hospital and the research for large scale epidemiology to handle sporadic affair.

Description

technical field [0001] The invention belongs to the field of biological detection, and in particular relates to a test strip for detecting influenza A and B virus antigens and an application thereof. Background technique [0002] At present, the detection of influenza A and B viruses uses tissue culture and double serum antibody detection as the "gold standard". However, this method is cumbersome and complicated to operate, and takes a long time. It does not play a guiding role in the timely detection, forecast and prevention of influenza. However, it still plays an important role in the analysis of genetic and antigenic changes in virus populations and in the discovery of new viruses. Commonly used methods for rapid detection of influenza include: direct and indirect fluorescence assay (IFA / DFA), enzyme immunoassay (EIA), enzyme activity assay (neuraminidase assay), APAAP bridged enzyme-labeled assay, nucleic acid amplification method (PCR), but there are problems such as ...

Claims

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Application Information

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IPC IPC(8): G01N33/569G01N33/558G01N21/78G01N33/547
Inventor 刘明
Owner BEIJING ZHUANGDI HAOHE BIOMEDICINE SCI & TECH
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