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Method for preparing anti-duck viral hepatitis transfer factor

A duck viral hepatitis and transfer factor technology, applied in the direction of antiviral agents, antiviral immunoglobulins, medical preparations containing active ingredients, etc., can solve the problem that duck viral hepatitis cannot be fundamentally prevented, and achieve the Duck viral hepatitis, the effect of simple and easy preparation method and short production time

Inactive Publication Date: 2011-01-26
河南后羿生物工程股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although there are already some drugs or preparations against duck viral hepatitis, they cannot fundamentally prevent duck viral hepatitis.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] This embodiment takes duck type 1 viral hepatitis as an example to prepare anti-duck viral hepatitis transfer factor. The steps of the method are as follows:

[0019] 1) Preparation of duck hepatitis virus antigen: Inoculate duck type 1 viral hepatitis in the allantoic cavity of 9-day-old chicken embryos for proliferation, culture at 33°C for 72 hours, take chicken embryo allantoic fluid, and put it into the sterilized In a centrifuge tube, ultrasonically sonicate for 10min (50Hz), centrifuge at 5000rpm / min for 20min, discard the precipitate and take the supernatant to obtain the crude virus antigen;

[0020] 2) Extraction of virus antigen: Carefully spread 20%, 30%, 40%, and 66% gradient sucrose solutions in sequence in centrifuge tubes, each gradient sucrose solution is 1ml, and finally thicken the antigen on the surface of the solution by 0.2 ml, after centrifugation at 40000r / min for 2.5h, there is a virus band on the second layer, carefully suck it out, dialyze to ...

Embodiment 2

[0027] This embodiment takes duck type 1 viral hepatitis as an example to prepare anti-duck viral hepatitis transfer factor. The steps of the method are as follows:

[0028] 1) Preparation of duck hepatitis virus antigen: inoculate duck type 1 viral hepatitis in the allantoic cavity of 9-day-old chicken embryos for proliferation, culture at 35°C for 84 hours, take chicken embryo allantoic fluid, and put it into sterilized chicken embryos In a centrifuge tube, ultrasonically sonicate for 10min (50Hz), centrifuge at 5000rpm / min for 20min, discard the precipitate and take the supernatant to obtain the crude virus antigen;

[0029] 2) Extraction of virus antigen: Carefully spread 20%, 30%, 45%, and 60% gradient sucrose solutions in sequence in centrifuge tubes, each gradient sucrose solution is 1ml, and finally thicken the antigen on the solution surface by 0.2 ml, after centrifugation at 40000r / min for 2.5h, there is a virus band on the second layer, carefully suck it out, dialyz...

Embodiment 3

[0036] This embodiment takes duck type 1 viral hepatitis as an example to prepare anti-duck viral hepatitis transfer factor. The steps of the method are as follows:

[0037]1) Preparation of duck hepatitis virus antigen: inoculate duck type 1 viral hepatitis in the allantoic cavity of 10-day-old chicken embryos for proliferation, culture at 37°C for 96 hours, take chicken embryo allantoic fluid, and put it into sterilized In a centrifuge tube, ultrasonically sonicate for 10min (50Hz), centrifuge at 5000rpm / min for 20min, discard the precipitate and take the supernatant to obtain the crude virus antigen;

[0038] 2) Extraction of virus antigen: Carefully spread 25%, 45%, 55%, and 65% gradient sucrose solutions in sequence in centrifuge tubes, each gradient sucrose solution is 1ml, and finally thicken the antigen on the solution surface by 0.2 ml, after centrifugation at 40000r / min for 2.5h, there is a virus band on the second layer, carefully suck it out, dialyze to remove the ...

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Abstract

The invention discloses a method for preparing an anti-duck viral hepatitis transfer factor, which comprises the following steps of: firstly, inoculating duck viral hepatitis in a chick embryo allantoic cavity for multiplication and extracting a virus antigen from the chick embryo allantoic cavity; secondly, immunizing a healthy pig body with the extracted virus antigen; and thirdly, extracting the anti-duck viral hepatitis transfer factor from the spleen of the immune pig. In the invention, specific transfer factor is extracted from duck viral hepatitis viruses and can effectively suppress the duck viral hepatitis viruses and protect the cells of a normal body from being infected with the duck viral hepatitis viruses, so that the duck viral hepatitis is prevented and treated radically. Moreover, the preparation method of the invention is simple and feasible, short in production time and low in cost and has a promising application prospect.

Description

technical field [0001] The invention relates to a preparation method of anti-duck viral hepatitis transfer factor. Background technique [0002] Duck viral hepatitis is a highly lethal viral infectious disease of ducklings. The disease was first discovered in the United States, and later found in many duck-raising countries such as the United Kingdom, Canada, and Germany. The disease is characterized by acute onset, rapid spread, high mortality, opisthotonus clinical manifestations, and cases of hepatitis and hemorrhage. The disease causes significant economic losses to duck farms. Although some medicines or preparations against duck viral hepatitis have been arranged at present, they cannot fundamentally prevent duck viral hepatitis. Contents of the invention [0003] The purpose of the present invention is to provide a preparation method of specific anti-duck viral hepatitis transfer factor to inhibit duck viral hepatitis virus, protect normal body cells from infection...

Claims

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Application Information

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IPC IPC(8): A61K35/28C07K16/08A61P31/12A61K35/26
Inventor 吴红云韩改会李建正
Owner 河南后羿生物工程股份有限公司
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