IgG kit for detecting streetvirus of dogs using indirect enzyme immunosorbent assay and preparation method thereof

A technology of adsorption test and rabies virus, which is applied in the field of indirect enzyme immunosorbent assay for detection of canine rabies virus IgG kit and its preparation, and can solve the problem that there is no commercial sale of the kit and no research report on the method kit for the detection of rabies virus antibody, etc. question

Inactive Publication Date: 2004-11-17
湖北省预防医学科学院
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Problems solved by technology

However, the method and corresponding test kit for the detection of rabies virus antibody in experimental animals (including dogs, monkeys, mice, etc.) have not yet seen the research report on the detection method of rabies virus antibody in experimental animals, nor have the corresponding test kits been commercially available.

Method used

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  • IgG kit for detecting streetvirus of dogs using indirect enzyme immunosorbent assay and preparation method thereof
  • IgG kit for detecting streetvirus of dogs using indirect enzyme immunosorbent assay and preparation method thereof

Examples

Experimental program
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Embodiment 1

[0077] 1 Preparation of HRP-rabbit anti-dog IgG

[0078] 1.1 Extraction and purification of canine IgG

[0079] 1.1.1 Choose one healthy and qualified Beagle dog over 6 months old who is negative for rabies virus antibody [license number is SCXK (E) 2003-0001]], collect blood from the heart or bloodletting from the carotid artery, and separate the serum.

[0080] 1.1.2 Take canine serum xml, add xml saline to dilute: add 2 xml saturated ammonium sulfate solution dropwise under stirring (final concentration is 50% saturated ammonium sulfate), set at 4°C for 30 minutes (flocculent precipitates appear), centrifuge at 3000rpm 30 minutes, remove the supernatant: dissolve the precipitate with 2xml normal saline, add xml saturated ammonium sulfate dropwise under stirring (final concentration is 33% saturated ammonium sulfate), place at 4°C for 30 minutes, centrifuge at 5000rmp for 30 minutes, remove the supernatant; PBS (0.01mol / L pH7.4) dissolves the precipitate, puts it into an an...

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Abstract

The invention refers to a kind of detecting reagent box and the manufacturing method, concretely refers to the reagent which is indirect enzyme immune sorption experiment for detecting rabies virus IgG and the manufacturing method. The reagent box compositions are: beforehand enclosed rabies virus antigen enzyme label board, sample diluting solution, HRP-rabies resisting IgG enzyme compound, condensed washer solvent, substrate and stopping liquid. The specificity of the reagent can reach 100%; the sensitivity is 1:640; the accuracy (the variation coefficient) is 6.98%. The reagent uses indirect ELISA to detect the rabies virus IgG antibody.

Description

technical field [0001] The invention relates to a detection kit and a preparation method thereof, in particular to a detection kit for canine rabies virus IgG by an indirect enzyme immunosorbent assay and a preparation method thereof. technical background [0002] Experimental animals are an important foundation and one of the necessary supporting conditions for life sciences. The quality of experimental animals is not only directly related to the success or failure of related scientific research work, but also an important symbol of a country's overall related scientific research level. The quality assurance of experimental animals not only depends on the level of feeding management and hardware conditions, but also depends on a standardized detection system, and the key to the detection system is science, advanced detection methods and standardized detection reagents. At present, my country's laboratory animal testing standards and systems are not complete, uniform, non-s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/535G01N33/543G01N33/569
Inventor 张瑜徐国景唐利军肖红雨孙凡中张金明易平杨文祥林乔龚镇奎
Owner 湖北省预防医学科学院
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