IgG kit for detecting streetvirus of dogs using indirect enzyme immunosorbent assay and preparation method thereof
A technology of adsorption test and rabies virus, which is applied in the field of indirect enzyme immunosorbent assay for detection of canine rabies virus IgG kit and its preparation, and can solve the problem that there is no commercial sale of the kit and no research report on the method kit for the detection of rabies virus antibody, etc. question
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[0077] 1 Preparation of HRP-rabbit anti-dog IgG
[0078] 1.1 Extraction and purification of canine IgG
[0079] 1.1.1 Choose one healthy and qualified Beagle dog over 6 months old who is negative for rabies virus antibody [license number is SCXK (E) 2003-0001]], collect blood from the heart or bloodletting from the carotid artery, and separate the serum.
[0080] 1.1.2 Take canine serum xml, add xml saline to dilute: add 2 xml saturated ammonium sulfate solution dropwise under stirring (final concentration is 50% saturated ammonium sulfate), set at 4°C for 30 minutes (flocculent precipitates appear), centrifuge at 3000rpm 30 minutes, remove the supernatant: dissolve the precipitate with 2xml normal saline, add xml saturated ammonium sulfate dropwise under stirring (final concentration is 33% saturated ammonium sulfate), place at 4°C for 30 minutes, centrifuge at 5000rmp for 30 minutes, remove the supernatant; PBS (0.01mol / L pH7.4) dissolves the precipitate, puts it into an an...
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