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Human vaccine for preventing hydrophobia and tetanus

A rabies vaccine and rabies technology, applied in the field of vaccines, can solve problems such as inconvenience of medication, and achieve the effects of convenient medication, increased titer, and enhanced immunity

Active Publication Date: 2012-09-19
CHENGDU KANGHUA BIOLOGICAL PROD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In addition, a large amount of tetanus antitoxin is usually injected after a rabies bite to prevent Clostridium tetani infection, which causes inconvenience in medication

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] one. Firstly, human diploid cell WI-38 is recovered, cultured and expanded to meet the needs of production batches. Animal cell culture medium 199 culture medium was used, 2 bovine serum and kanamycin 26U / ml were added to the cell culture medium, pH 7.0, the culture temperature was 35°C, and the culture method was spinner bottle culture.

[0038] During the culture process, the cell separation rate is determined according to the needs of the production batch. In this example, 1:2 is selected. When the cells grow into a dense monolayer, the rabies virus can be inoculated, and 0.3% (W / W) is added to the cell maintenance solution. At the same time, adjust the pH to 7.0, culture at 35°C, and add an appropriate amount of antibiotics. When inoculating, first wash the cell surface with a culture medium such as Earle's solution to remove residual bovine serum, and then grow dense cells Inoculate 0.1 mol of rabies virus strain CDKHBP-1 strain on the human diploid cells of the l...

Embodiment 2

[0045] one. Firstly, human diploid cell WI-38 is recovered, cultured and expanded to meet the needs of production batches. Animal cell culture medium 199 culture medium was used, 10% bovine serum and gentamicin 30U / ml were added to the cell culture medium, pH 7.5, the culture temperature was 37°C, and the culture method was microcarrier reactor culture.

[0046] During the culture process, the cell sorting rate is determined according to the needs of the production batch. In this embodiment, 1:4 is selected. When the cells grow into a dense monolayer, the rabies virus can be inoculated. It is best to add 0.5% (W / W) human serum albumin, adjust the pH to 7.5 at the same time, culture at 37°C, and add an appropriate amount of antibiotics. The culture medium that can be used includes 199 liquid, constant salt solution, etc. The inoculation process includes first using a culture medium such as Earle's solution Wash the cell surface to remove residual bovine serum, then inoculate ...

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PUM

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Abstract

The invention discloses a human vaccine for preventing hydrophobia and tetanus, which belongs to the field of vaccines. The vaccine consists of a hydrophobia vaccine and a tetanus toxin, wherein the hydrophobia vaccine is obtained by inoculating a CDKHBP-1 strain onto a human diploid cell WI-38 and purifying. By using the hydrophobia vaccine and the tetanus toxin prepared with the preparation method disclosed by the invention together, immunity to hydrophobia and tetanus can be realized; and moreover, the tetanus toxin can be used for remarkably enhancing valence effect of the hydrophobia vaccine, plays a role in enhancing immunity, and is convenient for administration.

Description

technical field [0001] The invention belongs to the field of vaccines, in particular to a human vaccine for preventing rabies and tetanus. Background technique [0002] Rabies, rabies, also known as hydrophobia, is an acute viral infectious disease that affects the central nervous system. All warm-blooded animals, including humans, may be infected. It is mostly obtained from the bite of infected animals. Since there is no effective treatment for rabies and the mortality rate is extremely high, vaccination is the only effective means to prevent the occurrence of rabies and ensure people's health. [0003] Human diploid cell (Human Diploid Cell, HDC) rabies vaccine is obtained by adapting the fixed strain of rabies virus on human diploid cells, subculture, proliferating, concentrating and purifying. Because the cells used are derived from humans, they have the advantages of small side effects, short immune onset time, and high antibody titers. Especially for rabies, a highly...

Claims

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Application Information

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IPC IPC(8): A61K39/295A61K39/205A61K39/08A61P31/04A61P31/14C12N7/00C12R1/93
Inventor 蔡勇侯文礼钟泽荣冯晓
Owner CHENGDU KANGHUA BIOLOGICAL PROD
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