Human vaccine for preventing hydrophobia and tetanus

A rabies vaccine and rabies technology, applied in the field of vaccines, can solve problems such as inconvenience of medication, and achieve the effects of convenient medication, enhanced immunity, and increased titer

Active Publication Date: 2013-08-14
CHENGDU KANGHUA BIOLOGICAL PROD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In addition, a large amount of tetanus antitoxin is usually injected after a rabies bite to prevent Clostridium tetani infection, which causes inconvenience in medication

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] One. The human diploid cell WI-38 is first recovered, cultured and expanded to meet the needs of production batches. Use animal cell culture medium 199 culture medium, add 2 bovine serum and kanamycin 26 U / ml to the cell culture medium, pH 7.0, the culture temperature is 35° C., and the culture method is roller bottle culture.

[0038] During the culture process, the cell division rate is determined according to the needs of the production batch. In this example, 1:2 is selected. When the cells grow into a dense monolayer, the rabies virus can be inoculated, and 0.3% (W / W) is added to the cell maintenance solution. At the same time adjust the pH 7.0, the culture temperature is 35 ℃, and an appropriate amount of antibiotics can be added. When inoculating, first wash the cell surface with culture medium such as Earle's solution to remove the residual bovine serum. The layer of human diploid cells are inoculated with 0.1 mol of the rabies virus strain CDKHBP-1 strain, and cu...

Embodiment 2

[0045] One. The human diploid cell WI-38 is first recovered, cultured and expanded to meet the needs of production batches. Use animal cell culture fluid 199 culture fluid, add 10% bovine serum and gentamicin 30U / ml to the cell culture fluid, pH 7.5, its culture temperature is 37 ℃, culture method is microcarrier reactor culture.

[0046] During the culture process, the cell division rate is determined according to the needs of the production batch. In this example, 1:4 is selected. When the cells grow into a dense monolayer, the rabies virus can be inoculated. It is best to add 0.5% (W / W) human albumin, adjust pH 7.5, culture temperature 37℃, and add appropriate amount of antibiotics. Usable culture medium includes 199 solution, constant salt solution, etc. The inoculation process includes first use of culture medium such as Earle's solution Wait for washing the cell surface to remove the remaining bovine serum, then inoculate 0.01 mol of the rabies virus strain CDKHBP-1 strai...

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PUM

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Abstract

The invention discloses a human vaccine for preventing hydrophobia and tetanus, which belongs to the field of vaccines. The vaccine consists of a hydrophobia vaccine and a tetanus toxin, wherein the hydrophobia vaccine is obtained by inoculating a CDKHBP-1 strain onto a human diploid cell WI-38 and purifying. By using the hydrophobia vaccine and the tetanus toxin prepared with the preparation method disclosed by the invention together, immunity to hydrophobia and tetanus can be realized; and moreover, the tetanus toxin can be used for remarkably enhancing valence effect of the hydrophobia vaccine, plays a role in enhancing immunity, and is convenient for administration.

Description

technical field [0001] The invention belongs to the field of vaccines, in particular to a human vaccine for preventing rabies and tetanus. Background technique [0002] Rabies, rabies, also known as hydrophobia, is an acute viral infectious disease that affects the central nervous system. All warm-blooded animals, including humans, may be infected. It is mostly obtained from the bite of infected animals. Since there is no effective treatment for rabies and the mortality rate is extremely high, vaccination is the only effective means to prevent the occurrence of rabies and ensure people's health. [0003] Human diploid cell (Human Diploid Cell, HDC) rabies vaccine is obtained by adapting the fixed strain of rabies virus on human diploid cells, subculture, proliferating, concentrating and purifying. Because the cells used are derived from humans, they have the advantages of small side effects, short immune onset time, and high antibody titers. Especially for rabies, a highly...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K39/295A61K39/205A61K39/08A61P31/04A61P31/14C12N7/00C12R1/93
Inventor 蔡勇侯文礼钟泽荣冯晓
Owner CHENGDU KANGHUA BIOLOGICAL PROD
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