Method for producing PRRS (Porcine Reproductive and Respiratory Syndrome) viruses

A production method and pig breathing technology, applied in biochemical equipment and methods, viruses/phages, microorganisms, etc., can solve the problems of being easily polluted by the environment, polluting the quality of vaccines, and high production costs, reducing production costs and achieving uniform product quality. , the effect of short production cycle

Active Publication Date: 2011-04-06
成都史纪生物制药有限公司
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  • Abstract
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Problems solved by technology

The traditional process is labor-intensive, time-consuming, low in efficiency, and high in production cost; it is easy to be polluted by the environment; there are large differences between different production batches or between different bottles of the same production batch; it is difficult to expand production; it is easy to be contaminated by bacteria or other viruses The hidden dangers of vaccine quality, involving biosafety and public health issues

Method used

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  • Method for producing PRRS (Porcine Reproductive and Respiratory Syndrome) viruses
  • Method for producing PRRS (Porcine Reproductive and Respiratory Syndrome) viruses

Examples

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Embodiment Construction

[0029] The present invention will be further described below in conjunction with the accompanying drawings and specific embodiments.

[0030] (1) Select a torrent-perfusion bioreactor as the means of cultivation: 10L torrent-perfusion bioreactor.

[0031] (2) Select the paper carrier as the carrier for cell attachment and growth: polyester amide paper carrier.

[0032] (3) Pretreatment method of the paper carrier: 1) soak the paper carrier with sterile PBS overnight; 2) wash the paper carrier 3 times with sterile PBS; 3) soak the paper carrier with cell culture growth solution for 2 hours above.

[0033] (4) Marc-145 cells were selected as cells for seedling production.

[0034] (5) Subculture and cultivation of cells for seedling production.

[0035] The above-mentioned cells were digested and passaged with trypsinized solution, and continued to be cultured with a cell growth medium containing 10% bovine serum, 1% double antibody (mixed solution of penicillin and streptomy...

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Abstract

The invention discloses a method for producing PRRS (Porcine Reproductive and Respiratory Syndrome) viruses through culturing Marc-145 cells by applying a torrent-perfusion bioreactor. The method comprises the following steps of: (1) selecting Marc-145 cells as cells for culturing seedlings; (2) subculturing the Marc-145 cells; (3) reproducing virus seeds for production; (4) perfusing and culturing the Marc-145 cells on a paper carrier in the torrent-perfusion bioreactor; (5) producing the PRRS viruses in the torrent-perfusion bioreactor; and (6) treating the obtained virus antigen solution. The invention can greatly decrease the production cost, improve the input-output ratio by more than 10 times and expand the production scale easily and rapidly, has short production preparation cycle and high degree of automation, occupies less area, causes less environmental pollution which is easy to treat, consumes less labor, is easy to realize balanced and stable virus quality and can obviously improve the yield and the quality of the viruses.

Description

technical field [0001] The invention relates to a vaccine production method using a torrent-perfusion bioreactor and paper carrier cell culture technology, which can be used for industrial production of porcine respiratory and reproductive syndrome vaccine and replaces the traditional spinner bottle culture production process. Background technique [0002] At present, the production of porcine respiratory and reproductive disorder syndrome vaccine in China mainly relies on the cell spinner bottle culture method. The traditional process is labor-intensive, time-consuming, low in efficiency, and high in production cost; it is easy to be polluted by the environment; there are large differences between different production batches or between different bottles of the same production batch; it is difficult to expand production; it is easy to be contaminated by bacteria or other viruses The hidden dangers of vaccine quality caused by this problem involve biosafety and public health...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N7/00C12N7/02
Inventor 徐宏军丁光星
Owner 成都史纪生物制药有限公司
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