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Preparation method for gill tissue paraffin section

A technology of paraffin section and production method, which is applied in the preparation of test samples, etc., can solve the problems of inconspicuous staining contrast between nucleus and cytoplasm, poor staining, unclear layers, etc., achieve good staining and observation effects, and improve clear structure Degree, enhance the effect of penetration

Inactive Publication Date: 2014-07-23
SHANXI AGRI UNIV
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Problems solved by technology

Due to the particularity of fish gill tissue structure, it is difficult to obtain ideal results by using traditional paraffin sectioning methods. Not only the structure is blurred, but also the staining contrast between the nucleus and cytoplasm is not obvious, the layers are unclear, and the coloring is poor, which leads to the preparation of tissue specimens. fail

Method used

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Embodiment 1

[0030] Experimental purpose: To observe the pathological changes of carp gill tissue after exposure to different concentrations of fluoride for 90 days by preparing paraffin sections of gill tissue. Specific steps are as follows:

[0031] (1) Preparation or purchase of reagents

[0032] A Preparation of Bouin’s fixative solution Prepared by volume ratio from the following reagents: 15 parts of saturated picric acid, 5 parts of formalin, and 1 part of glacial acetic acid.

[0033] B Preparation of Perenyi’s decalcification solution It is prepared by volume of the following reagents: 4 parts of 10% nitric acid, 3 parts of 0.5% chromic acid, and 3 parts of absolute ethanol.

[0034] C Choose paraffin wax with a melting point of 56~58°C for flaky slices.

[0035] (2) Sampling Carps exposed to different concentrations of fluoride for 90 days were anesthetized in an ice bath, and then the second piece of gill on the left side was cut, with a volume of 3 mm×3 mm×2 mm. Two samples ...

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Abstract

The invention discloses a preparation method for a gill tissue paraffin section. The preparation method comprises the following steps: fixing, decalcifying, dehydrating, transparentizing, carrying out paraffin permeation, embedding, slicing, sticking sections, expanding the sections, de-waxing and rehydrating, staining, re-staining, sealing and the like. Compared with an existing paraffin section manufacturing method, an operation process of dehydrating, transparentizing and immersing by wax is improved; the preparation fixing and tissue wax immersing effects of gill tissue paraffin are improved; a slicing problem when the gill tissue paraffin section is prepared is improved; the structure definition of the gill tissue section is greatly improved; a plurality of problems in a gill tissue manufacturing process in the prior art are solved. The preparation method is good for antigen positioning of immunocytochemical staining, so that when experiments including in-situ hybridization, immunohistochemistry, immunofluorescence and the like are carried out on the gill tissue paraffin section, tissue distribution and cell positioning of some genes and proteins can be displayed, and further feasible conditions are provided for carrying out gill research on levels of cells, genes and proteins.

Description

technical field [0001] The invention belongs to the field of microscopic tissue sections, and in particular relates to a method for preparing fish gill tissue paraffin sections. Background technique [0002] Paraffin section is the most widely used method in routine histological preparation techniques. Paraffin section is not only used to observe the morphological structure of normal cells and tissues, but also the main method for studying, observing and judging the morphological changes of cells and tissues in pathology and forensic science, and has been widely used in research in many other disciplines middle. In teaching, most of the section samples observed under the light microscope are prepared by paraffin section method. Living cells or tissues are mostly colorless and transparent, and there is a lack of contrast between various tissues and various structures in the cells, and it is difficult to distinguish them clearly under a general light microscope; tissues will...

Claims

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Application Information

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IPC IPC(8): G01N1/28G01N1/30G01N1/36
Inventor 曹谨玲陈剑杰罗永巨王俊东李宏全宋晶
Owner SHANXI AGRI UNIV
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