Preparation method for gill tissue paraffin section
A technology of paraffin section and production method, which is applied in the preparation of test samples, etc., can solve the problems of inconspicuous staining contrast between nucleus and cytoplasm, poor staining, unclear layers, etc., achieve good staining and observation effects, and improve clear structure Degree, enhance the effect of penetration
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[0030] Experimental purpose: To observe the pathological changes of carp gill tissue after exposure to different concentrations of fluoride for 90 days by preparing paraffin sections of gill tissue. Specific steps are as follows:
[0031] (1) Preparation or purchase of reagents
[0032] A Preparation of Bouin’s fixative solution Prepared by volume ratio from the following reagents: 15 parts of saturated picric acid, 5 parts of formalin, and 1 part of glacial acetic acid.
[0033] B Preparation of Perenyi’s decalcification solution It is prepared by volume of the following reagents: 4 parts of 10% nitric acid, 3 parts of 0.5% chromic acid, and 3 parts of absolute ethanol.
[0034] C Choose paraffin wax with a melting point of 56~58°C for flaky slices.
[0035] (2) Sampling Carps exposed to different concentrations of fluoride for 90 days were anesthetized in an ice bath, and then the second piece of gill on the left side was cut, with a volume of 3 mm×3 mm×2 mm. Two samples ...
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