66 results about "Tissue Distributions" patented technology

Described herein are compositions and methods of use of anti-pancreatic cancer antibodies or fragments thereof, such as murine, chimeric, humanized or human PAM4 antibodies. The subject antibodies show a number of novel and useful therapeutic characteristics, such as binding with high specificity to pancreatic and other cancers, but not to normal or benign pancreatic tissues and binding to a high percentage of early stage pancreatic cancers. In preferred embodiments, the antibodies bind to pancreatic cancer mucins. The antibodies and fragments are of use for the detection, diagnosis and / or treatment of cancer, such as pancreatic cancer. The antibodies, such as PAM4 antibodies, bind to a PAM4 antigen that shows unique cell and tissue distributions compared with other known antibodies such as CA19.9, DUPAN2, SPAN1, Nd2, B72.3, and Lea and Le(y) antibodies that bind to the Lewis antigens.

The present invention relates to a novel compound of use in the improved delivery of therapeutic drug agents into target cells or tissues, composition comprising the same and uses thereof. The compound is more specifically a conjugate of a peptide moiety and a camptothecin, a derivative or analog thereof which provides numerous benefits, including enhancement in terms of aqueous solubility, pharmacokinetics and tissue distribution, enlargement of the therapeutic index, and limitation of the inter-patient metabolic variability, as well as improvement of delivery of the biologically active ingredient to the target cells or tissues.

Highly efficient cationic liposomes have been developed as an improved delivery system for biologically-active reagents. A novel structure, the sandwich liposome, is formed and comprises one or more biologically active agents internalized between two bilomellar liposomes. This structure protects the incoming agent and accounts for the high efficiency of in vivo delivery and for the broad tissue distribution of the sandwich liposome complexes.These novel liposomes are also highly efficient carriers of nucleic acids. By using extruded DOTAP:cholesterol liposomes to form complexes with DNA encoding specific proteins, expression has been improved dramatically. Highest expression was achieved in the lung, while increased expression was detected in several organs and tissues.

The invention discloses a plate shape correction method during steel plate quenching based on model control. Firstly, according to the warping direction and warping amount of the steel plate shape after actual quenching, calculating the difference of the steel plate tissue distribution by utilizing an established steel plate surface tissue distribution model; then obtaining the cooling speed difference of upper and lower surfaces of the steel plate by utilizing an established steel plate surface temperature model according to the tissue distribution difference of the upper and lower surfaces of the steel plate; and finally, calculating the heat transfer coefficient ratio of the upper and lower surfaces of the steel plate by utilizing an established water volume optimization model and regulating the water volume and distribution of subsequent steel plates quantitatively to control the plate shape.

A geldanamycin derivate with nucleoside base is prepare by inducing soluble nucleoside by geldanamycin and obtaining the final product. Nucleoside base is connected with geldanamycin on Linker. It can keep or strengthen antiviral activity of geldanamycin and improve solubility and tissue distribution.

The invention discloses a preparation method for a gill tissue paraffin section. The preparation method comprises the following steps: fixing, decalcifying, dehydrating, transparentizing, carrying out paraffin permeation, embedding, slicing, sticking sections, expanding the sections, de-waxing and rehydrating, staining, re-staining, sealing and the like. Compared with an existing paraffin section manufacturing method, an operation process of dehydrating, transparentizing and immersing by wax is improved; the preparation fixing and tissue wax immersing effects of gill tissue paraffin are improved; a slicing problem when the gill tissue paraffin section is prepared is improved; the structure definition of the gill tissue section is greatly improved; a plurality of problems in a gill tissue manufacturing process in the prior art are solved. The preparation method is good for antigen positioning of immunocytochemical staining, so that when experiments including in-situ hybridization, immunohistochemistry, immunofluorescence and the like are carried out on the gill tissue paraffin section, tissue distribution and cell positioning of some genes and proteins can be displayed, and further feasible conditions are provided for carrying out gill research on levels of cells, genes and proteins.

The present invention relates to a novel compound of use in the improved delivery of therapeutic drug agents into target cells or tissues, composition comprising the same and uses thereof. The compound is more specifically a conjugate of a peptidic moiety and a camptothecin, a derivative or analog thereof which provides numerous benefits, including enhancement in terms of aqueous solubility, pharmacokinetics and tissue distribution, enlargement of the therapeutic index, and limitation of the inter-patient metabolic variability, as well as improvement of delivery of the biologically active ingredient to the target cells or tissues.

The invention belongs to the technical field of medicines and relates to a myricetin nanosuspension and a preparation method thereof. The myricetin nanosuspension is prepared by adopting a settling method and a high-pressure homogenization method, and the formula comprises myricetin and a stabilizer according to the weight ratio of 1: 0.25-1: 2.5. According to the preparation method provided by the invention, the formula is optimized, Tween-80, Cremophor EL and D-alpha-tocopheryl polyethylene glycol 1000 succinate (TPGS) are screened as the stabilizer, the prepared myricetin nanosuspension has stable nature and simple composition of the formula, the preparation process is simple, convenient and feasible, and the particle size range is 100nm-300nm; and by reducing the particle size of a medicament, the solubility and the dissolution of the myricetin are obviously improved, and the oral bioavailability and the in-vivo tissue distribution of the myricetin are further improved. In addition, the myricetin nanosuspension can be freeze-dried, and proper excipients are added into the obtained freeze-dried powder for further preparing oral liquid, tablets, granules, capsules and other different oral preparations so as to facilitate clinical applications.

The method provides a method for measuring a volume of dairy cow mammary tissue. The method includes the following steps of placing two shooting devices at a plurality of preset positions on two sides of a fence respectively, calibrating the shooting devices, collecting images as original images, acquiring a dairy cow breast image, constructing a three-dimensional description of a breast shape, calculating an upper bound depth and a lower bound depth of a mammary tissue distribution area of each breast area, acquiring three-dimensional coordinates of upper and lower bounds of the mammary tissue distribution area in a normal vector direction of an external three-dimensional point cloud, constituting inside and outside three-dimensional descriptions of the cow mammary tissue distribution area, acquiring the ratio of mammary tissue in an ultrasound image of each breast area, and calculating an actual volume of the mammary tissue of each breast area. According to the method for measuring the volume of the dairy cow mammary tissue, a cow breast three-dimensional model is constructed by means of a computer vision technology, measurement of the appearance assessment index data and volume is performed, internal tissue distribution of organs are analyzed through the ultrasound image, and real-time measurement of a proportional relation of the internal tissue distribution of cow breasts is performed.

The invention belongs to the technical field of medical image intelligent processing, and particularly relates to a cancer full-view digital pathological section segmentation method based on a local classification neural network, which comprises the following steps of: dividing a pathological section into a plurality of image blocks which have fixed sizes and are not overlapped; sending each imageblock into a classification model based on a convolutional neural network, judging the category of the block through forward propagation, and splicing the classification results of the blocks according to the positions of the blocks on the original image, thereby obtaining a heat map of different tissue distribution in the whole slice; finally, conducting median filtering on the heat map, removing an area with a too small area, so as to obtain a segmentation image and assist a doctor in diagnosis. By means of the method, the areas where different tissues in the whole pathological section arelocated can be rapidly marked, doctors are effectively assisted in diagnosis, and the diagnosis accuracy and efficiency are improved.

The invention belongs to the technical field of medicines, and in particular relates to a nanosuspension of annonaceous acetogenin drugs prepared from such amphiphilic stabilizers as mPEG PCL, mPEG PLA, mPEG PLGA, mPEG DSPE, mPEG Chol, SPC, Tween80, BSA, TPGS and the like, as well as a preparation method and an application of the nanosuspension. The annonaceous acetogenin total lactone nanosuspension is prepared by virtue of a solvent precipitation-ultrasonic injection method, and according to a prescription, the proportioning ratio of the annonaceous acetogenin drugs to the stabilizer is at 1 to (0.02-10) (in percentage by weight). The prepared annonaceous acetogenin nanosuspension can reach a load-loading capacity to 90% to the greatest extent and can reach a minimum grain size to 123.2nm, and the nanosuspension is good in polydispersity. The nanosuspension is stable in both gastrointestinal fluid and plasma, and the nanosuspension is applicable to oral administration and injection administration; the nanosuspension has a good in-vitro sustained-release effect and is free from burst release; the nanoparticle, in comparison with a crude drug solution, is more obvious in tumor cell inhibitory rate in vitro; the nanosuspension, in in-vivo tissue distribution, shows tumor passive targeting; therefore, the nanosuspension is beneficial for enhancing efficacy and reducing toxic and side effects. Meanwhile, an in-vivo efficacy experiment shows that the nanosuspension is outstanding in antineoplastic efficacy, and the nanosuspension has a broad development prospect.

Nucleic acid compositions encoding a pro-apoptotic protein, Bok (Bcl-2-related ovarian killer) are identified. Bok has conserved Bcl-2 homology domains 1, 2 and 3 and a C-terminal transmembrane region present in other Bcl-2 related proteins, but lacks the BH4 domain found only in anti-apoptotic Bcl-2 proteins. Over-expression of Bok induces apoptosis. Cell killing induced by Bok is suppressed by co-expression with selective anti-apoptotic Bcl-2 proteins. Bok is highly expressed in the ovary, testis and uterus, particularly in granulosa cells, the cell type that undergoes apoptosis during follicle atresia. Identification of Bok as a new pro-apoptotic protein with wide tissue distribution and hetero-dimerization properties facilitates elucidation of apoptosis mechanisms in reproductive and other tissues, and provides a means for manipulating apoptosis.

The invention discloses a DNA sequence for coding spodoptera exigua vitellogenin (SEVg). The invention also discloses a specific polypeptide sequence capable of utilizing Western blot to detect the AlVg protein expression level. The invention also discloses a polyclonal antibody of an SEVg polypeptide and a preparation method thereof. The polypeptide specific antibody is utilized for analyzing the tissue distribution of SEVg in spodoptera exigua and the difference expression on the protein level. The invention can be used for observing and predicting the population dynamics of spodoptera exigua in fields and can provide material basis and technical support for quick detection and molecular biology study of the protein.

The invention relates to an adherent peptide capable of specifically combining hepatocarcinoma tissue / cell, screening method of said adherent peptide and application of said adherent peptide, belonging to the field of molecular pharmacology technology. The internal and external combined screening method provided by said invention has the advantages of internal screening method and external screening method, and can overcome their defects, and two kinds of hepatocarcinoma tissue / cell adherent peptides can be screened out, and their polypeptide sequences ore determined. Said hepatocarcinoma tissue / cell specific adherent peptides have good guide effect and strong tissue distribution specificity, and have broad application prospects in preparation of guide medicine for curing hepatocarcinoma.

The invention provides application of an aminoadamantane mononitrate compound in preparation of drugs for preventing and treating diseases such as glaucoma, vascular dementia, subarachnoid hemorrhage and pulmonary arterial hypertension. Discovered by experiments, the aminoadamantane mononitrate compound can be combined with an NMDA receptor and release nitric oxide (NO), excitatory toxicity of nerve cells induced by glutamic acid can be lowered, the compound plays a role in dilating cerebral blood vessels, cerebral blood flow can be improved, but peripheral blood pressure is not lowered; retinal ganglion cell injury induced by high intraocular pressure can be reduced, and the intraocular pressure is lowered through releasing the NO; in a vascular dementia model, the cerebral blood flow can be improved, motor behavior disorder is relieved, and learning cognitive ability is improved; behavioral abnormality and cerebrovascular spasm induced by subarachnoid hemorrhage can be relieved; discovered by researches on tissue distribution of the drugs, MN-08 can be gathered in pulmonary tissue, in a pulmonary arterial hypertension model, pulmonary blood vessels can be dilated, right ventricular pressure can be lowered, and plumpness of right ventricle can be reduced.

The invention discloses a quenching method of a major-diameter thick-wall pressure gas cylinder. Based on a numerical simulation technique, under the premise that a major-diameter thick-wall pressure gas cylinder can obtain satisfactory tissues and an inner cavity can release pressure completely and according to the temperature change of characteristic nodes of the body of the pressure gas cylinder and distribution of tissues, the invention provides a quenching and cooling method in which intermittent mist spraying is carried out on the inner surface of the gas cylinder and continuous water spraying is carried out on the outer surface of the gas cylinder when the gas cylinder rotates. The gas cylinder rotates to improve the cooling uniformity of the gas cylinder. The inner surface of the gas cylinder is cooled in a mist spraying manner to reduce the temperature difference of the inner and outer surfaces of the cylinder body so as to reduce the quenching stress, improve the hardenability and reduce the deformation of the cylinder body. The mist spraying pause process of the inner surface is beneficial to good pressure release effect of vapor in the inner cavity of the gas cylinder. By means of the method disclosed by the invention, the quenched major-diameter thick-wall pressure gas cylinder can obtain good tissue distribution, small deformation and residual stress, so that the quenching quality is improved.

The invention belongs to the technical field of preparation of cold-rolled dual-phase steel, and relates to cold-rolled dual-phase steel DP780 and a flexible production method thereof. The cold-rolleddual-phase steel DP780 comprises the components according to the percentage by weight: 0.13 to 0.17 of C, 0.10 to 0.40 of Si, 1.0 to 1.2 of Mn, 0.01 to 0.04 of Ti, 0.10-0.20 of Cr, 0.02-0.06 of Al, less than or equal to 0.015 of P, less than or equal 0.010 of S, and the balance being Fe and inevitable impurities. The cold-rolled dual-phase steel DP780 prepared by the method has uniform tissue distribution, martensite volume fraction of less than 20%, average grain size of 3-4 micrometers, yield strength of 400-650 MPa, tensile strength of greater than or equal to 780 MPa, elongation percentage A80 of greater than or equal to 18%, n10-20 / Ag value of greater than or equal to 0.14, r90 value of greater than or equal to 0.8, and BH2 of greater than or equal to 50 MPa and can meet the requirements of automobile factories on individualization and high forming property.

The invention discloses a fluorescent selective histone deacetylase inhibitor and a preparation method and application thereof. The fluorescent selective histone deacetylase inhibitor has a structuralgeneral formula (I) or (II) show in the description. The compound of formula (I) or (II) structure is applicable to the preparation of drugs to detect tissue distribution of histone deacetylase and cell and tissue imaging, and drugs to treat or diagnose diseases related to histone deacetylase dysfunction.

The invention relates to a device for strengthening a laser cladding layer through high-frequency vibration rolling and a using method thereof, and belongs to the field of metal material surface strengthening. The device comprises a laser cladding system and an ultrasonic rolling system; the laser cladding system comprises a robot arm and a laser cladding head; and the ultrasonic rolling system comprises an ultrasonic rolling system support, an ultrasonic vibration device, a pressing roller and the like. Ultrasonic rolling is carried out while the laser cladding layer is formed, and the pressing roller acts on the edge of a molten pool. Ultrasonic vibration applied to the pressing roller can refine cladding layer grains and optimize the mobility of metal in the molten pool, so that tissue distribution is uniform, bubbles are easy to escape, and microdefects are reduced. The rolling of the pressing roller in an action area not only can realize surface grain refinement on a semi-solidified or just solidified cladding layer, convert residual tensile stress into residual compressive stress and improve the comprehensive performance of the cladding layer, but also can enable the surface of the cladding layer to be smooth and tidy, and can meet the surface quality and size requirements of most parts.

The invention belongs to the technical field of medical image intelligent processing, and particularly relates to a DCNN-based cancer full-view digital pathological section survival analysis method. The method comprises the following steps: respectively taking a plurality of image blocks from a lymphatic dense region, a necrosis region and a tumor region in a pathological section, sending the image blocks and global segmentation of the section into a convolutional neural network, and acquiring survival time distribution of the section through forward propagation The death probability of the patient in a plurality of time intervals can be obtained from the predicted survival time distribution, and the survival probability of the patient before a plurality of time nodes can be obtained through accumulation. Local images of the sections and global tissue distribution serve as input, the survival time distribution of the patient serves as output, a doctor is helped to estimate the prognosis condition of the patient, and therefore early clinical diagnosis of cancer is assisted.

Highly efficient cationic liposomes have been developed as an improved delivery system for biologically-active reagents. A novel structure, the sandwich liposome, is formed and comprises one or more biologically active agents internalized between two bilomellar liposomes. This structure protects the incoming agent and accounts for the high efficiency of in vivo delivery and for the broad tissue distribution of the sandwich liposome complexes.These novel liposomes are also highly efficient carriers of nucleic acids. By using extruded DOTAP:cholesterol liposomes to form complexes with DNA encoding specific proteins, expression has been improved dramatically. Highest expression was achieved in the lung, while increased expression was detected in several organs and tissues.

The invention provides a method for preparing ultra-high hardness cladding layer by synchronous ultrasonic vibration assisted laser technology. The specific scheme is to introduce ultrasonic vibration synchronously in the process of cladding Ni-based cermet coating, and this method can improve the flow of liquid metal In the process of solidification, the growing dendrite network can be broken and dispersed to various parts of the melt to form evenly distributed small crystal nuclei to prevent segregation of cermets, thereby It ensures that the cladding layer does not crack while obtaining ultra-high hardness; the equipment is simple, it is convenient for calibration and installation, the energy consumption is small, and the working environment is good. The obtained coating cermet has uniform particle distribution, high hardness and good wear resistance.

The invention relates to an analysis method and an analysis system of a tissue structure of a woven fabric, belonging to the technical field of image recognition. The analysis method comprises the following steps of: receiving a fabric image; roughly adjusting the warp and latitude trend; identify the distribution of different tissues on the fabric to obtain the distribution area of a single tissue in the image; at least a single tissue block slice is cut from each single tissue distribution area; obtain the cyclic pattern and number of cycles of the tissue structure in a single tissue block slice; calculating the overall warp and weft density of the fabric to be analyzed according to the warp and weft density of all weave structures on the fabric to be analyzed; output Results. By dividing the fabric image into tissue slices of single tissue, and then recognizing the slices with artificial intelligence, the fabric texture can be identified accurately, and the fabric warp and weft density value can be obtained, so that the subsequent lofting copies of fabric products can be directly completed by computer, and can be widely used in the field of textile manufacturing.

The invention discloses an Apolygus lucorum soluble trehalase (AlTre-1) monoclonal antibody as well as a preparation method and application of thereof. The method comprises the following steps: firstly, applying AlTre-1 recombinant protein subjected to prokaryotic expression and purification to an immunized mice so as to prepare antiserum, and determining the titer of the antiserum by an indirect ELISA method; and preparing myeloma cells and spleen cells and then performing cell fusion, screening positive porocyte by an ELISA method, then performing subcloning, and picking out positive monoclonal holes for enlarging cultivation and strain fixing, thereby finally obtaining the AlTre-1 monoclonal antibody. Besides, a Western blot method determines that the monoclonal antibody can specifically identify the AlTre-1 recombinant protein and can generate specific immune reaction. By virtue of the application of the AlTre-1 monoclonal antibody, a material base and a technical support can be provided for the tissue distribution of soluble trehalase in Apolygus lucorum and the quick detection and molecular biology study of the protein.

Described herein are compositions and methods of use of anti-pancreatic cancer antibodies or fragments thereof, such as murine, chimeric, humanized or human PAM4 antibodies. The subject antibodies show a number of novel and useful therapeutic characteristics, such as binding with high specificity to pancreatic and other cancers, but not to normal or benign pancreatic tissues and binding to a high percentage of early stage pancreatic cancers. In preferred embodiments, the antibodies bind to pancreatic cancer mucins. The antibodies and fragments are of use for the detection, diagnosis and / or treatment of cancer, such as pancreatic cancer. The antibodies, such as PAM4 antibodies, bind to a PAM4 antigen that shows unique cell and tissue distributions compared with other known antibodies such as CA19.9, DUPAN2, SPAN1, Nd2, B72.3, and Lea and Le(y) antibodies that bind to the Lewis antigens.

The invention provides a herbal mixture extractive of angelica keiskei and folium ginkgo capable of reducing insulin resistance. The extractive is prepared by extracting raw angelica keiskei and raw folium ginkgo based on weight ratio of 1:(0.1-10); and the extractive mainly comprises total flavone from angelica keiskei and total flavone from folium ginkgo. The invention also provides a preparation method of the extractive. The extractive shows remarkable hypoglycemic effect while being applied to a diabetic mice model and an insulin resistance rat model, and also can influence the tissue distribution and the function level of in-situ intestinal incretin secretory cells, and therefore, the insulin resistance can be treated; and the extractive can be developed and prepared into a medicine for reducing and treating insulin resistance, and thus the application of the extractive in preparation of a medicine for reducing the insulin resistance is provided.