Preparation method of paraffin section of fish gill tissue

A technology of paraffin section and production method, which is applied in the preparation of test samples, etc., can solve the problems of inconspicuous staining contrast between nucleus and cytoplasm, poor staining, unclear layers, etc., achieve good staining and observation effects, and improve clear structure Degree, enhance the effect of penetration

Inactive Publication Date: 2016-02-24
SHANXI AGRI UNIV
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AI Technical Summary

Problems solved by technology

Due to the particularity of fish gill tissue structure, it is difficult to obtain ideal results by using traditional paraffin sectioning methods. Not only the structure is blurred, but also the staining contrast between the nucleus and cytoplasm is not obvious, the layers are unclear, and the coloring is poor, which leads to the preparation of tissue specimens. fail

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Experimental purpose: To observe the pathological changes of carp gill tissue after exposure to different concentrations of fluoride for 90 days by preparing paraffin sections of gill tissue. Specific steps are as follows:

[0031] (1) Preparation or purchase of reagents

[0032] A Preparation of Bouin's fixative solution is prepared by volume ratio of the following reagents: 15 parts of saturated picric acid, 5 parts of formalin, and 1 part of glacial acetic acid.

[0033] B Preparation of Perenyi’s decalcification solution is prepared by volume of the following reagents: 4 parts of 10% nitric acid, 3 parts of 0.5% chromic acid, and 3 parts of absolute ethanol.

[0034] C choose and buy scale-shaped paraffin wax with a melting point of 56-58 °C.

[0035] (2) Sampling Anesthetize carp exposed to different concentrations of fluoride for 90 days in an ice bath, then cut out the second piece of gill on the left, with a volume of 3mm×3mm×2mm, take 2 samples from each grou...

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Abstract

The invention discloses a method for making paraffin slices of fish gill tissue, including fixation, decalcification, dehydration, transparency, paraffin penetration, embedding, slicing, sticking and spreading, dewaxing and rehydrating, dyeing, counterstaining and sealing and other steps. Compared with the existing method for making paraffin slices, the present invention improves the operation process of dehydration, transparency, and wax immersion, improves the effects of preparation and fixation of fish gill tissue paraffin wax and tissue wax immersion, and improves the slicing problem during preparation of fish gill tissue paraffin slices. The structure definition of the fish gill tissue section is greatly improved, and several problems existing in the production process of the fish gill tissue in the prior art are solved. It is beneficial to the antigen localization of immunocytochemical staining, so in situ hybridization, immunohistochemistry and immunofluorescence experiments on fish gill tissue sections can show the tissue distribution and cellular localization of certain genes and proteins. The study of fish gills at the protein level provides a feasible condition.

Description

technical field [0001] The invention belongs to the field of microscopic tissue sections, and in particular relates to a method for preparing fish gill tissue paraffin sections. Background technique [0002] Paraffin section (paraffinsection) is the most widely used method in routine histological preparation techniques. Paraffin section is not only used to observe the morphological structure of normal cells and tissues, but also the main method for studying, observing and judging the morphological changes of cells and tissues in pathology and forensic science, and has been widely used in research in many other disciplines middle. In teaching, most of the section samples observed under the light microscope are prepared by paraffin section method. Living cells or tissues are mostly colorless and transparent, and there is a lack of contrast between various tissues and various structures in the cells, and it is difficult to distinguish them clearly under a general light micros...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N1/28G01N1/30G01N1/36
Inventor 曹谨玲陈剑杰罗永巨王俊东李宏全宋晶
Owner SHANXI AGRI UNIV
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