Tumor necrosis factor-alpha induced protein 8 L3 (TIPE3) immunohistochemistry detection kit for diagnosing lung cancer

A technology of immunohistochemistry and detection kits, which is applied in the field of medical and biological detection, can solve the problems of low positive rate of lung cancer, low specificity, and increased number of patients, and achieve the goal of improving the quality of life, strong specificity, and increased survival rate Effect

Inactive Publication Date: 2012-10-03
SHANDONG UNIV
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Problems solved by technology

[0003] In recent years, in the auxiliary diagnosis of lung cancer, the study of tumor markers is very active. Currently, the clinically used diagnostic markers for lung cancer mainly include CEA, SCC, CYFRA21 1, etc., but they still have many limitations: many tumor cells can CEA is produced, and in some benign tumors and non-tumor diseases, some patients c

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  • Tumor necrosis factor-alpha induced protein 8 L3 (TIPE3) immunohistochemistry detection kit for diagnosing lung cancer
  • Tumor necrosis factor-alpha induced protein 8 L3 (TIPE3) immunohistochemistry detection kit for diagnosing lung cancer
  • Tumor necrosis factor-alpha induced protein 8 L3 (TIPE3) immunohistochemistry detection kit for diagnosing lung cancer

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Embodiment 1

[0027] Example 1 A Tipe3 immunohistochemical detection kit for diagnosing lung cancer

[0028] The contents of the kit include: natural peroxidase and biotin inactivator, non-specific protein blocking agent, rabbit anti-human primary antibody and antibody diluent, horseradish peroxidase-labeled goat anti-rabbit monoclonal Cloning linking antibody, chromogenic reagent, and cell nucleus staining agent, each reagent should be added in sequence during operation, and the volume ratio of the amount used is: endogenous peroxidase and biotin inactivator: non-specific protein blocking agent: initial antibody : Antibody Diluent: Conjugated Antibody: Chromogenic Reagent: Cell Nuclear Stain = 100:100:0.5:100:100:200:100. According to the actual use, each reagent can be divided into 20, 50, and 100 doses of reagents in each box according to the above ratio.

[0029] The following is the composition of the lung cancer TIPE3 immunohistochemical diagnostic kit prepared by 50 copies per box: ...

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Abstract

The invention discloses a tumor necrosis factor-alpha induced protein 8 L3 (TIPE3) immunohistochemistry detection kit for diagnosing lung cancer. Contents of the kit comprise a reagent which is used for inactivating endogenous peroxidase and biotin in a human tissue section, a non-specific protein blocking agent which is used for blocking non-specific staining of cross protein reaction in the human tissue section, a rabbit anti-human initial antibody which is combined with human tissue antigen, an antibody diluent, a goat anti-rabbit monoclonal connection antibody which can be connected with the initial antibody and is marked by horse radish peroxidase, a developing reagent which can be reacted with the horse radish peroxidase and used for developing, and a reagent which can specifically stain nuclei in tissues. TIPE3 is positively expressed in various kinds of pathological type lung cancer, tumors can be early detected and found, the kit is high in specificity and high in sensitivity and can be applied to early diagnosis of the lung cancer, a patient can be effectively and timely treated, unnecessary medical cost is reduced, the survival quality of the patient is improved, survival time is prolonged, and the survival rate of the patient who suffers from the lung cancer is improved.

Description

technical field [0001] The invention relates to a Tipe3 immunohistochemical detection kit for diagnosing lung cancer, which belongs to the field of medical and biological detection. Background technique [0002] Lung cancer is the most common primary malignant tumor of the lung. In recent years, the morbidity and mortality of lung cancer have been on the rise in countries all over the world, especially in industrialized countries, and it has become an important factor in global cancer deaths. It is called " The world's number one cancer killer". In recent years, the incidence of lung cancer in my country has risen rapidly. In 2000, there were 1.8-2 million new cases, 1.4-1.5 million deaths, and an incidence rate of 36.7 / 100,000. The total number of lung cancer patients ranks first in the world. The high mortality rate of lung cancer is mainly due to the insidious onset of lung cancer and the lack of effective screening and early diagnosis methods, which delays the best time...

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Application Information

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IPC IPC(8): G01N33/577G01N33/532G01N1/30
Inventor 张利宁王群刘香岚石永玉郭春朱法良王晓燕王嘉宁
Owner SHANDONG UNIV
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