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Rapid frozen section method for camphor leaf

A technology of frozen sections and blades, applied in the direction of sampling devices, etc., can solve the problem of easy formation of ice crystals

Inactive Publication Date: 2015-11-25
XUZHOU UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to overcome the above-mentioned deficiencies in the prior art and solve the problem of easily forming ice crystals in the frozen section of camphor tree plant tissue, the present invention adopts the combination of direct embedding freezing of plant material and proper return temperature, and provides a kind of quick frozen section method of camphor tree leaf

Method used

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  • Rapid frozen section method for camphor leaf
  • Rapid frozen section method for camphor leaf
  • Rapid frozen section method for camphor leaf

Examples

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Embodiment

[0024] Such as figure 1 , figure 2 and image 3 Shown, a kind of camphor tree leaf quick frozen section method, the cryostat used in this method is Lycra CM3050S, and it comprises microtome cooling switch 1, slicer cabinet 2, machine head 3, rotary lever 4, freezing platform 5; Press as follows Steps to prepare:

[0025] Step 1) Immediately put the cut leaves of camphor tree into 4% paraformaldehyde fixative solution, fix at 4°C for 2-3 hours, soak 2-3 times with 100mmol / L phosphate buffer solution, and obtain Camphor tree leaf samples, spare.

[0026] Step 2) Turn on the cooling switch 1 of the slicer, the temperature of the slicer box 2 is maintained at -20°C, and the temperature of the freezing table 5 is lowered to -20°C. The camphor tree leaf sample obtained through step 1) is evenly spread with OCT frozen section embedding agent, so that the camphor tree leaf sample is deeply buried in it, the embedding is completed, and it is rapidly cooled and fixed on the sample...

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Abstract

The present invention provides a rapid frozen section method for camphor leaf. The method is as below: fixing fresh cut camphor leaf at 4 DEG C for 2-3 h; maintaining the temperature of box and freezing station at -20 DEG C; evenly smearing a material sample with an OCT frozen section embedding agent to embed the sample deeply in the agent; rapidly cooling and fixing the sample on a sample stage; shaking a rotating lever outside the machine body, and trimming to an observation site; then taking the sample stage along with the embedded block out of a slicer; placing at room temperature for 20-30 s to soften the embedded block; quickly placing the embedded block into the a slicer, and slicing in 15-20 s, wherein the slice thickness is 10-20 mum; gently touching the embedding agent around the section by using a dissecting needle, so as to attach the section to the dissecting needle; and transferring the section to a slide glass outside the slicer by the dissecting needle and fully expanding the section. Fully expanded section generally does not need special treatment for direct dyeing or tissue chemical analysis and observation and photographing under the microscope.

Description

technical field [0001] The invention belongs to the technical field of microtissue sectioning, and relates to the production of plant frozen sections, in particular to a method for quick frozen sectioning of camphor leaves. Background technique [0002] camphor tree ( Cinnamomum camphora ) is an important tree species in the subtropical evergreen broad-leaved forest in China. It is mainly distributed in the provinces south of the Yangtze River Basin. Important raw material tree species for chemical industry and medicine. It is also one of the tree species with the greatest economic value in the evergreen trees of the Lauraceae genus, and has great potential for development and utilization. [0003] As the global climate tends to warm, camphor trees are becoming more and more popular in northern cities as an excellent landscaping tree species. The research on the cold resistance of camphor tree is very important for the successful introduction and application of camphor tr...

Claims

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Application Information

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IPC IPC(8): G01N1/06
Inventor 黄绍辉刘艳
Owner XUZHOU UNIV OF TECH
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