Building method and application of zebra fish hyperlipidemia model

Abstract

The invention relates to a building method of a zebra fish hyperlipidemia model and application of the animal model to hyperlipidemia disease research and lipid-lowering drug screening. The building method of the zebra fish hyperlipidemia model mainly includes the steps of zebra fish selection, feeding of zebra fish by yolk powder, histochemical staining or fluorescent staining, image analysis and/or microwell plate analysis and statistical analysis. The building method has the advantages of simplicity, convenience, rapidity, economy, high efficiency, high throughput and the like, and the model can be used for hyperlipidemia disease research and lipid-lowering drug screening. The building method and application of the zebra fish bacterial infection model are of great significance to acceleration of research and development on lipid-lowering drugs and improvement on treatment of patients suffering from hyperlipidemia.

Description

technical field [0001] The invention belongs to the field of biological model construction, and in particular relates to a method for establishing a zebrafish hyperlipidemia model and an application thereof. Background technique [0002] The disease in which one or more lipids in plasma is higher than normal due to abnormal fat metabolism or operation is called hyperlipidemia. Hyperlipidemia is a systemic disease, refers to blood cholesterol (TC) and / or triglyceride (TG) is too high or high-density lipoprotein cholesterol (HDL-C) is too low, modern medicine is called dyslipidemia [1] . [0003] A large number of epidemiological surveys and studies have shown that hyperlipidemia is the most important risk factor for the onset of atherosclerosis, coronary heart disease and cerebrovascular disease. Hyperlipidemia is also an important risk factor for hypertension, impaired glucose tolerance, and diabetes. Hyperlipidemia can also lead to fatty liver, liver cirrhosis, cholelith...

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Problems solved by technology

Baek et al. [8] Feed zebrafish with a high-cholesterol diet to establish a high-cholesterol model, but the establishment of a high-cholesterol model in this article takes a long time, and the cholesterol analysis uses lysed zebrafish, which is cumbersome and prone to false positives or false negatives, which is not suitable for lipid-lowering drugs filter

Clifton et al. [9] Zebrafish fluorescent dyes were used to screen new fat absorption inhibitors. In the article, the author mentioned that zebrafish were treated with egg yolk powder and drugs, and then fat staining was performed to observe the effect of drugs on fat absorption inhibition; in this article, the author did not establish The zebrafish hyperlipidemia model only observes the experimental results qualitatively, and does not establish a quantitative evaluation method for pharmacodynamics

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