A method for constructing a zebrafish bacterial infection model and its application
A construction method, a technology of bacterial infection, applied in applications, fish farming, climate change adaptation, etc., can solve problems such as differences in physiological structure and physiological functions, poor prediction reliability and comparability, and large differences in biological levels, etc., to achieve repeatability Stable and reliable performance, simple experimental process operation, high success rate effect
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[0050] The object of the present invention is to provide a construction method and application of a zebrafish bacterial infection model. The method provided by the invention has the advantages of simplicity, rapidity, economy, high efficiency, high throughput and the like.
[0051] One, the present invention provides a kind of method that establishes zebrafish bacterial infection model, design scheme is:
[0052] 1. Culture of Bacteria
[0053] Transfer the red fluorescent plasmid RFP(Kan+) with kanamycin resistance into chloramphenicol-resistant Escherichia coli BL21(DE3)Plyss(Chl+), after successful transformation, apply LB solid plates (Kan+, Chl+) for screening Escherichia coli (hereinafter referred to as BL21RFP) capable of producing red fluorescence and having kanamycin resistance and chloramphenicol resistance, after growing a single colony, store the plate at 4°C. One day before each zebrafish bacterial injection, a single colony of BL21RFP was picked and placed in L...
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