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Establishment method and application of zebrafish multiple sclerosis model

A technology for multiple sclerosis and method establishment, applied in the establishment of zebrafish multiple sclerosis model, and in the field of nerve myelin damage, can solve the problems that the treatment is not necessarily effective, and achieve intuitive, comparable, and simple experiments Effects of Process Operations

Active Publication Date: 2013-08-07
HANGZHOU HUANTE BIOLOGICAL TECH CO LTD
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

However, the transgenic fluorescent zebrafish is a normal myelin zebrafish, which is completely different from the pathological changes of demyelination in MS. Therefore, the myelin formation promoter screened by the normal transgenic fluorescent zebrafish with myelin is not necessarily effective for the treatment of MS. efficient

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  • Establishment method and application of zebrafish multiple sclerosis model
  • Establishment method and application of zebrafish multiple sclerosis model
  • Establishment method and application of zebrafish multiple sclerosis model

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Embodiment Construction

[0068] The purpose of the present invention is to provide a method for establishing a living zebrafish multiple sclerosis model, and at the same time provide a method for screening multiple sclerosis therapeutic drugs and evaluating compound-induced myelin damage by using the model. The method provided by the invention has the advantages of simplicity, speed, economy, high efficiency, high throughput and the like.

[0069] 1. The present invention provides a method for establishing a live zebrafish multiple sclerosis model, the design scheme is:

[0070] 1Zebrafish selection

[0071] Take 4~5 pairs of zebrafish parents for mating, according to Westerfield [33] Method of incubating embryos. Place the zebrafish at the optimal stage for observation under a dissecting microscope, pick out the normal zebrafish and move them into 6, 12, 24, 48, 96 or 384-well microplates, and put different quantities according to the microplate specifications Zebrafish.

[0072] 2 compound treatment

[007...

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Abstract

The invention relates to an establishment method for a zebrafish multiple sclerosis model, and a method for screening therapeutic medicines for multiple sclerosis or evaluating compound-induced nerve myelin injuries by using the animal model. The establishment method of the zebrafish multiple sclerosis model mainly comprises the following steps of: zebrafish selection, compound treatment, whole zebrafish immunofluorescent staining, image analysis and / or microplate selection, and statistical analysis. The method disclosed by the invention has the advantages of being economic, simple and convenient, rapid, efficient, less in the dosage of compound and the like, can realize a purpose of screening the therapeutic medicines for multiple sclerosis with a high flux in vivo, and is significant for acceleration for the research and development process of the therapeutic medicines for multiple sclerosis, and therapy for patients with multiple sclerosis.

Description

Technical field [0001] The invention belongs to the field of drug screening (evaluation), and specifically relates to a simple, economical, and high-throughput method for establishing a zebrafish multiple sclerosis model, and uses the animal model to screen multiple sclerosis therapeutic drugs or evaluate compounds induced Methods of nerve myelin injury. Background technique [0002] Multiple sclerosis (MS) is an autoimmune disease characterized by white matter demyelination of the central nervous system. It may be a neuroimmune process that occurs in genetically susceptible individuals and environmental factors, due to its high incidence , Has a chronic course and tends to be affected by young people, and becomes one of the most important neurological diseases [1] . Epidemiology shows that there are about 400,000 cases of the disease in North America, about 65,000 cases in my country, and more than 1 million cases in the world. There are more female patients than men, and it mo...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/473A61P37/02G01N21/64
Inventor 李春启朱晓宇朱凤郭胜亚
Owner HANGZHOU HUANTE BIOLOGICAL TECH CO LTD
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