The invention discloses a method for extracting adipose-derived stem cells, and belongs to the technical field of stem cell extraction. The method for extracting the adipose-derived stem cells comprises the steps of fat acquisition, washing, digestion, cleaning, lysis of red blood cells, termination of lysis, culture, re-inoculation, passage and identification. According to the method, adipose tissue is washed repeatedly with a cell washing liquid to effectively remove fat droplets; a digestive enzyme is utilized to digest collagenous fiber in an intercellular substance to release the cells, so that the damage to the cells is small, and the free cells maintain the activity of the cells in a mesenchymal basal culture medium; subculture is carried out on the cells not attached to walls in asupernatant of a cell culture bottle, thereby increasing the survival rate of the cells, and increasing the multiplication rate of the cells. The method for extracting the adipose-derived stem cells has the advantages that the method is simple and convenient; the enzymolysis of the adipose tissue is thorough, and each component cell is released maximumlly; the survival rate of the obtained adipose-derived stem cells is high.