Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for purifying edible fungus polysaccharide by using enzyme pretreatment-microwave assisted extraction-chitosan flocculation technology

A microwave-assisted and enzymatic pretreatment technology is applied in the field of extracting and purifying active ingredients, which can solve the problems of large amount of ethanol, long production cycle, low extraction efficiency, etc., and achieve the effects of convenient process, simplified operation, and enhanced extraction effect.

Inactive Publication Date: 2015-04-08
EAST CHINA UNIV OF SCI & TECH
View PDF6 Cites 8 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Traditional extraction techniques, such as percolation, Soxhlet extraction, maceration, and reflux extraction, generally have disadvantages such as low extraction efficiency, high energy consumption, and long production cycle; the purification method is commonly used by water extraction and alcohol precipitation, which has a long production cycle. , large amount of ethanol used and other disadvantages

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Extraction and Purification of Flammulina velutipes Polysaccharides from Flammulina velutipes

[0025] (1) Reflux the dried Flammulina velutipes in ethanol for 2 hours according to the material-to-liquid ratio of 1:10, and dry them with hot air at 65°C;

[0026] (2) Weigh 5g of pretreated Flammulina velutipes, put it in a three-neck flask, add 125mg of pectinase, and then add 125mL of deionized water, mix and enzymolyze in a water bath at 40°C for 45min;

[0027] (3) Transfer the mixture in step (2) to a microwave extraction device, irradiate for 150s at a microwave power of 650W, cool to room temperature, and filter under reduced pressure to obtain a crude extract of Flammulina velutipes polysaccharide, with a polysaccharide yield of 11.35%;

[0028] (4) Concentrate the crude extract of Flammulina velutipes polysaccharides in step (3) according to the concentration ratio of 10:1, adjust the pH to 2.0, add 4mL of 1% chitosan solution, keep the temperature at 35°C, flocc...

Embodiment 2

[0033] Extraction and Purification of Grifola frondosa Polysaccharides from Grifola frondosa

[0034] (1) Reflux the dried Grifola frondosa in ethanol for 2 hours according to the material-to-liquid ratio of 1:10, dry it with hot air at 65°C, and take the grifola frondosa particles with a particle size of 10-20 mesh after crushing;

[0035] (2) Weigh 5g of pretreated Grifola frondosa, put it in a three-necked flask, and add 150mL of deionized water with a pH of 6;

[0036] (3) Transfer the mixture in step (2) to a microwave extraction device, irradiate at a microwave power of 650W for 210s, cool to room temperature, and filter under reduced pressure to obtain a crude extract of Grifola frondosa polysaccharide, with a polysaccharide yield of 22.61% ;

[0037] (4) Concentrate the crude frondosa frondosa polysaccharide extract in step (3) according to the concentration ratio of 4:1, adjust the pH to 6.0, add 5mL of 1% chitosan solution, keep the temperature at 45°C, and floccula...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
particle size (mesh)aaaaaaaaaa
Login to View More

Abstract

The invention relates to a method for purifying edible fungus polysaccharide by using an enzyme pretreatment-microwave assisted extraction-chitosan flocculation technology. The method comprises the following steps: adding enzyme into crushed edible fungi, mixing with water, stirring, and performing enzymolysis for a certain period of time; performing microwave radiation for a period of time, then cooling and performing suction filtration to obtain a polysaccharide extract; concentrating the extract, then adding chitosan to perform flocculation, and filtering to remove deposition after flocculation is finished, thus obtaining a purified polysaccharide solution; and drying to obtain relatively pure edible fungus polysaccharide which is tested to have good vitro antioxidation activity. According to the method provided by the invention, an enzymolysis technology is adopted to perform a certain degree of hydrolysis destruction on cell walls and intercellular substances of edible fungi at first, and then a 'body heating' characteristic of microwave is adopted to promote the dissolution of active ingredients. The method is efficient and energy-saving and can reduce the solvent consumption; and chitosan serving as a natural flocculating agent can well remove protein high polymer impurities, is good in impurity removal effect and low in cost, and cannot influence the product quality.

Description

technical field [0001] The invention relates to a method for extracting and purifying active ingredients from edible fungi. Background technique [0002] In recent years, natural active ingredients in plants have become one of the important means for human beings to find new drugs. Edible fungus polysaccharides have anti-oxidation, anti-tumor and immune regulation effects, and have potential development value. However, due to the low content of active ingredients, how to effectively obtain high-purity edible fungus polysaccharides is an important issue for technology researchers. [0003] Traditional extraction techniques, such as percolation, Soxhlet extraction, maceration, and reflux extraction, generally have disadvantages such as low extraction efficiency, high energy consumption, and long production cycle; the purification method is commonly used by water extraction and alcohol precipitation, which has a long production cycle. , Ethanol usage is large and other disadva...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
Inventor 韩伟罗文锋满宁韩乐马迪豆欣欣汪佳丹
Owner EAST CHINA UNIV OF SCI & TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com