Method for purifying edible fungus polysaccharide by using enzyme pretreatment-microwave assisted extraction-chitosan flocculation technology

Abstract

The invention relates to a method for purifying edible fungus polysaccharide by using an enzyme pretreatment-microwave assisted extraction-chitosan flocculation technology. The method comprises the following steps: adding enzyme into crushed edible fungi, mixing with water, stirring, and performing enzymolysis for a certain period of time; performing microwave radiation for a period of time, then cooling and performing suction filtration to obtain a polysaccharide extract; concentrating the extract, then adding chitosan to perform flocculation, and filtering to remove deposition after flocculation is finished, thus obtaining a purified polysaccharide solution; and drying to obtain relatively pure edible fungus polysaccharide which is tested to have good vitro antioxidation activity. According to the method provided by the invention, an enzymolysis technology is adopted to perform a certain degree of hydrolysis destruction on cell walls and intercellular substances of edible fungi at first, and then a 'body heating' characteristic of microwave is adopted to promote the dissolution of active ingredients. The method is efficient and energy-saving and can reduce the solvent consumption; and chitosan serving as a natural flocculating agent can well remove protein high polymer impurities, is good in impurity removal effect and low in cost, and cannot influence the product quality.

Description

technical field

[0001] The invention relates to a method for extracting and purifying active ingredients from edible fungi. Background technique

[0002] In recent years, natural active ingredients in plants have become one of the important means for human beings to find new drugs. Edible fungus polysaccharides have anti-oxidation, anti-tumor and immune regulation effects, and have potential development value. However, due to the low content of active ingredients, how to effectively obtain high-purity edible fungus polysaccharides is an important issue for technology researchers.

[0003] Traditional extraction techniques, such as percolation, Soxhlet extraction, maceration, and reflux extraction, generally have disadvantages such as low extraction efficiency, high energy consumption, and long production cycle; the purification method is commonly used by water extraction and alcohol precipitation, which has a long production cycle. , Ethanol usage is large and other disadva...