Biological 3d printed active biological membrane for improving AMIC technology cartilage repair and preparation method thereof

An active biofilm and cartilage repair technology, applied in tissue regeneration, medical science, prosthesis, etc., can solve the problems of incompatibility of allogeneic bone reconstruction, different size, shape and curvature, abnormal intra-articular stress, etc., and achieve the ability to significantly promote cartilage. , good histocompatibility and biodegradability, and the effect of promoting cartilage regeneration

Active Publication Date: 2020-08-28
NANJING FIRST HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] For large-scale talus osteochondral injuries, the most common method at present is to use allogeneic bone transplantation after the lesion is removed. When the allogeneic bone is taken, the three-dimensional geometry of the amputated recipient's talus lesion is artificially observed and the corresponding part is cut from the allogeneic talus. For repair and reconstruction, this method has strong subjectivity and inaccuracy, and the shape of the talus is irregular, and the size, shap...

Method used

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  • Biological 3d printed active biological membrane for improving AMIC technology cartilage repair and preparation method thereof
  • Biological 3d printed active biological membrane for improving AMIC technology cartilage repair and preparation method thereof
  • Biological 3d printed active biological membrane for improving AMIC technology cartilage repair and preparation method thereof

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Experimental program
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Effect test

Embodiment 1

[0060] 1) Printing material preparation

[0061] 1.1) Aseptic treatment: gelatin, sodium alginate, hyaluronic acid, and calcium chloride powder were sterilized by ultraviolet light for 24 hours, and a magnetic heating stirrer was placed on an ultra-clean bench to prepare a hydrogel pre-preparation solution. All other operations are performed on a clean bench.

[0062] 1.2) Preparation of gelatin solution: Heat 2.5g of gelatin with 20ml of deionized water in a 48°C water bath, stir with a constant temperature magnetic stirrer at a speed of 300r / min, and let it fully dissolve to prepare a gelatin solution.

[0063] 1.3) Preparation of sodium alginate / gelatin solution: Add 20ml of deionized water and 1.25g of sodium alginate to the gelatin solution, heat in a water bath at 48°C with a rotation speed of 300r / min, and fully dissolve it into a sodium alginate / gelatin solution.

[0064] 1.4) Preparation of sodium alginate / gelatin / hyaluronic acid mixed solution: add 10ml of deionized...

Embodiment 2

[0073] 1) Printing material preparation

[0074] 1.1) Aseptic treatment: gelatin, sodium alginate, hyaluronic acid, and calcium chloride powder were sterilized by ultraviolet light for 24 hours, and a magnetic heating stirrer was placed on an ultra-clean bench to prepare a hydrogel pre-preparation solution. All other operations are performed on a clean bench.

[0075] 1.2) Preparation of gelatin solution: Heat 4g of gelatin in a 48°C water bath with 20ml of deionized water, stir with a constant temperature magnetic stirrer at a speed of 300r / min, and allow it to fully dissolve to prepare a gelatin solution.

[0076] 1.3) Preparation of sodium alginate / gelatin solution: Add 20ml of deionized water and 1.25g of sodium alginate to the gelatin solution, heat in a water bath at 48°C with a rotation speed of 300r / min, and fully dissolve it into a sodium alginate / gelatin solution.

[0077] 1.4) Preparation of sodium alginate / gelatin / hyaluronic acid mixed solution: add 10ml of deionize...

Embodiment 3

[0086] 1) Printing material preparation

[0087] 1.1) Aseptic treatment: gelatin, sodium alginate, hyaluronic acid, and calcium chloride powder were sterilized by ultraviolet light for 24 hours, and a magnetic heating stirrer was placed on an ultra-clean bench to prepare a hydrogel pre-preparation solution. All other operations are performed on a clean bench.

[0088] 1.2) Preparation of gelatin solution: Heat 5g of gelatin with 20ml of deionized water in a water bath at 48°C, stir with a constant temperature magnetic stirrer at a speed of 300r / min, and allow it to fully dissolve to prepare a gelatin solution.

[0089] 1.3) Preparation of sodium alginate / gelatin solution: Add 20ml of deionized water and 1.25g of sodium alginate to the gelatin solution, heat in a water bath at 48°C with a rotation speed of 300r / min, and fully dissolve it into a sodium alginate / gelatin solution.

[0090] 1.4) Preparation of sodium alginate / gelatin / hyaluronic acid mixed solution: add 10ml of dei...

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Abstract

The invention discloses a biological 3d printed active biological membrane of improved AMIC for cartilage repair. The method is characterized in that: sodium alginate, gelatin and hyaluronic acid areused as raw materials to prepare mixed hydrogel, cartilage precursor cells and fibronectin are mixed into the hydrogel, a deposition type biological 3D printing technology is used for constructing theporous hydrogel biological membrane, and chemical crosslinking is achieved through calcium chloride soaking to enhance the mechanical property. The materials used in the invention are all natural materials, are low in immunogenicity, good in biocompatibility and wide in source, have certain mechanical properties and can provide good support for cartilage regeneration. In terms of the preparationmethod, gaps of 300-500 microns can promote cartilage regeneration, meanwhile, an excellent porosity structure and the like can also promote intercellular substance exchange and communication, facilitate adhesion of cytokines or cells is, and facilitate growth and proliferation of the cells in the structure.

Description

technical field [0001] The invention relates to the technical field of biomedical materials, in particular to a bio-3D-printed modified AMIC active biofilm for cartilage repair and a preparation method thereof. Background technique [0002] Because the hyaline cartilage of the joint lacks the control of blood vessels, nerves, and lymph, it is difficult to achieve self-healing and easily develop into degenerative diseases once damaged. The existing clinical treatment techniques such as osteochondral transplantation, autologous chondrocyte transplantation, microfracture technology and Restorative effects such as AMIC technology are limited and the long-term curative effect is not good. In recent years, tissue engineering technology and bio-3D printing technology are gradually becoming a new generation of cartilage repair technology. 3D printing technology can precisely control the internal structure of the scaffold, construct a structure similar to cartilage morphology, and c...

Claims

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Application Information

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IPC IPC(8): A61L27/20A61L27/22A61L27/38A61L27/50A61L27/52A61L27/56B33Y70/10B33Y80/00
CPCA61L27/20A61L27/222A61L27/227A61L27/3817A61L27/50A61L27/52A61L27/56A61L2300/252A61L2300/412A61L2430/06B33Y70/00B33Y80/00C08L5/04C08L5/08
Inventor 桂鉴超周杨蒋逸秋秦然陈通
Owner NANJING FIRST HOSPITAL
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