New application of cells matched with tumor cell developmental stage and extract of cells

A technology for tumor cells and developmental stages, applied in antineoplastic drugs, drug combinations, pharmaceutical formulations, etc., can solve the problems that the common biological characteristics of cancer cells cannot be explained

Inactive Publication Date: 2015-09-09
齐锦生
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Although there are various carcinogens, the biological characteristics of tumor cells are the same, and after removing carcinogens, tumor cells still survive, so the treatment for etiology is meaningle

Method used

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  • New application of cells matched with tumor cell developmental stage and extract of cells
  • New application of cells matched with tumor cell developmental stage and extract of cells
  • New application of cells matched with tumor cell developmental stage and extract of cells

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] 9-11d chicken embryo liver extract can significantly inhibit the proliferation of HepG2

[0050] Under sterile conditions, take chicken embryos at different development stages and divide them into control group, 3-5d, 6-8d, 9-11d, 12-14d groups, add twice the volume of medium, and homogenize the embryos , after centrifugation, the supernatant was taken, passed through a 0.22 μm filter membrane, added to the HepG2 tumor cell culture medium (30% final concentration), and photographic observation and index detection were carried out after acting for different times.

[0051] The results showed that the proliferation of HepG2 cells in the 9-11d group and the cancer marker AFP were significantly inhibited with statistical significance (pfigure 1 , figure 2 , image 3 , Figure 7 )

Embodiment 2

[0053] 13.5-14.5d mouse embryonic liver co-culture can significantly inhibit the proliferation of HepG2

[0054] Under sterile conditions, mouse embryonic livers at different developmental stages were collected and divided into control group, 12.5d, 13.5d, 14.5d, 15.5d groups. After the primary cultured mouse embryonic liver cells were identified, they were co-cultured with liver cancer HepG2 cells. HepG2 cells (10 5 ) were grown on the bottom of a 6-well plate, embryonic liver cells (10 7 ) were co-cultured in the Transwell chamber. After co-cultivation for different periods of time, photographic observation and indicator detection were carried out.

[0055] The results showed that most HepG2 cells in the 13.5-14.5d group became round, shrunken and floated, while only a small part of adherent cells. In the 13.5-14.5d group, the proliferation rate of HepG2 and the cancer marker AFP were significantly inhibited, with statistical significance (p Figure 4 , Figure 5 , Ima...

Embodiment 3

[0057] Embryonic liver cells or their extracts promote the maturation of HepG2

[0058] The experimental treatment of chicken embryo fluid was the same as in Example 1, and the experimental treatment of mouse embryonic liver cells was the same as in Example 2. After 48 hours, Real time PCR was used to detect the gene expression of liver cancer proliferation marker gene Myc and liver cell differentiation marker gene HNF-4α.

[0059] The results showed that in the 9-11d chicken embryo fluid group and the 13.5-14.5d mouse embryonic liver cell co-culture group, the expression of Myc gene was significantly inhibited, while the expression of HNF-4α gene was significantly increased, which was statistically significant (p Figure 8 with Figure 9)

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Abstract

The invention discloses a new application of cells matched with a tumor cell developmental stage and an extract of the cells, that is, an application of the cells and the extract in preparation of medicines for inhibiting growth or proliferation of tumor cells. The cells matched with the tumor cell developmental stage comprise cells in all stages of the differentiation process from stem cells to mature cells or the embryonic development process; the extract of the cells comprises cell lysate, secretions, intercellular substances and the like. The functional mechanism is as follows: the tumor cells are induced to realize differentiation and maturation through the cells matched with the tumor cell developmental stage and the extract of the cells, so that growth or proliferation of the tumor cells is inhibited, and the purpose of curing tumor is achieved. The tumor cells are induced to realize differentiation and maturation and prevented from growing indefinitely through the cells matched with the tumor cell developmental stage and/or the extract of the cells, and finally, the tumor is cured.

Description

technical field [0001] The invention relates to a medicine for treating cancer, in particular to a new application of cells and extracts matching the developmental stage of tumor cells. Background technique [0002] After years of hard work, tumor mortality has not significantly decreased (Siegel R, Naishadham D, Jemal A. Cancer statistics, 2013. CA Cancer J Clin 2013; 63:11-30.2 Forouzanfar MH, Foreman KJ, Delossantos AM, et al . Breast and cervical cancer in 187 countries between 1980 and 2010: a systematic analysis. Lancet 2011; 378:1461-84.). Although the current treatment can effectively kill tumor cells, it cannot cure the tumor, nor can it suppress the recurrence, and the quality of life of the patient is very poor due to severe side effects. Therefore, finding an effective treatment is still a difficult problem in the medical field. To cure tumors, we must re-understand the origin and nature of tumor cells. [0003] Through the statistical analysis of tumor inciden...

Claims

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Application Information

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IPC IPC(8): A61K35/545A61K35/57A61P35/00
Inventor 齐锦生
Owner 齐锦生
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