Method for purifying herbaceous plant polysaccharide by adopting enzyme method extraction integrated flocculation technology
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A herbal and enzymatic extraction technology is applied in the field of extracting and purifying plant polysaccharides, which can solve the problems of long production cycle, low extraction efficiency, large alcohol consumption, etc. Effect
Inactive Publication Date: 2015-04-29
EAST CHINA UNIV OF SCI & TECH
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Problems solved by technology
The traditional extraction technology generally has disadvantages such as low extraction efficiency, high energy consumption, and long production cycle; the traditional purification method-alcohol precipitation method has problems such as long process flow, large alcohol consumption, and high cost.
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Embodiment 1
[0023] (1) Grind the dried Achyranthes bidentata to 20 mesh, reflux in industrial ethanol for 1 hour according to the ratio of material to liquid 1:10, cool to room temperature, and dry the dregs with hot air at 75°C after suction filtration;
[0024] (2) Weigh 5g of pretreated Achyranthes bidentata, put it in a round bottom flask, add 250mL of deionized water with a pH of 6, then add 20mg of cellulase, mix well, and enzymolyze it in a water bath at 45°C for 75min ;
[0025] (3) Heat the mixture obtained in step (2) to reflux, extract for 30 minutes, cool in a cold water bath after cooling at room temperature for a period of time, and filter under reduced pressure to obtain an extract containing Achyranthes bidentata polysaccharide, the polysaccharide yield 69.62%;
[0026] (4) Concentrate the Achyranthes bidentata polysaccharide extract obtained in step (3) according to the concentration ratio of 10:1, adjust the pH to 4, add 2mL of 1% chitosan solution under stirring, keep ...
Embodiment 2
[0029] (1) Grind the dried Achyranthes bidentata to 30 mesh, reflux in industrial ethanol for 1 hour according to the ratio of solid to liquid 1:10, cool to room temperature, and dry the dregs with hot air at 75°C after suction filtration;
[0030] (2) Weigh 3g of pretreated Achyranthes bidentata, put it in a round bottom flask, add 150mL of deionized water with a pH of 5, then add 24mg of cellulase, mix well, and enzymolyze it in a water bath at 45°C for 60min ;
[0031] (3) Heat the mixture obtained in step (2) to reflux, extract for 60 minutes, cool in a cold water bath after cooling at room temperature for a period of time, and filter under reduced pressure to obtain an extract containing Achyranthes bidentata polysaccharide, the polysaccharide yield 70.72%;
[0032] (4) Concentrate the Achyranthes bidentata polysaccharide extract obtained in step (3) according to the concentration ratio of 8:1, adjust the pH to 4, add 1.2mL of 1% chitosan solution under stirring, and kee...
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Abstract
The invention relates to a method for purifying herbaceous plant polysaccharide by adopting an enzyme method extraction integrated flocculation technology. The method comprises the following steps: adding an enzyme in to medicinal material particles obtained by crushing, stirring and mixing with water, and performing enzymolysis for a certain period of time; heating the obtained mixture until refluxing, extracting for a period of time, cooling to room temperature, and then performing reduced pressure suction filtration to obtain an extract containing polysaccharide; and concentrating the extract, adding a chitosan solution while stirring to perform flocculation purification, then centrifuging to obtain a purified polysaccharide solution, and drying to obtain herbaceous plant polysaccharide with relatively high purity. According to the method provided by the invention, a biological enzyme extraction technology is adopted to perform a certain degree of hydrolytic destruction on cell walls and intercellular substances of a herbaceous plant, the extraction efficiency of the method is high, and the activity of polysaccharide can be kept to the maximum extent; and a selected flocculant is a food additive namely chitosan which is approved by FDA, so that protein type high polymer impurities can be well eliminated, the purification effect is good, and the cost is low to ensure that whole production link is economic, environment-friendly and safe.
Description
technical field [0001] The invention relates to a method for extracting and purifying plant polysaccharides from herbs, in particular to a method for extracting and purifying polysaccharides from the traditional Chinese medicine Achyranthes bidentata. Background technique [0002] In recent years, studies have found that polysaccharides have pharmacological effects such as enhancing the body's immune function and anti-tumor. They can be used as active ingredients in various medicines and health products, and have great potential for the prevention and treatment of various diseases including cancer. [0003] Cyathula officinalis Kuan is one of the authentic medicinal materials produced in Sichuan. It is the dried root of Cyathula officinalis Kuan, a plant in the family Amaranthaceae. Modern pharmacological studies have shown that the main chemical components in Achyranthes bidentata are ecdysterones, saponins, isoflavones, fatty acids and polysaccharides, etc. Among them, Ach...
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