Method for preparing biological fertilizer by utilizing Alteromonas colwelliana A321 to ferment enteromorpha

A kind of technology of biological fertilizer and prolifera, which is applied in the field of biological fertilizer preparation by using Alteromonas colwelliana A321 to ferment prolifera

Active Publication Date: 2013-10-16
OCEAN UNIV OF CHINA
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Problems solved by technology

There are no relevant reports and patents about the fermentation of Enteromorpha enteromorpha by bacteria related to this patent

Method used

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Embodiment Construction

[0007] Production method of Alteromonas colwelliana A321

[0008] 1. Slant culture: Under sterile conditions, inoculate the original strain of Alteromonas colwelliana A321 on a solid medium, and culture at 28°C for 3 days.

[0009] 2. Seed culture: inoculate the bacteria cultured in step 1 into the liquid medium under aseptic conditions, and cultivate them on a shaker at 28° C. and 200 r / min for 3 days. The OD600 of the bacterial suspension of Alteromonas colwelliana A321 reached 3.0.

[0010] 3. Strain cultivation: The volume ratio of the liquid medium is 8% inoculum, the seed liquid is inoculated in a fermenter, and cultured at 28° C. and 200 r / min for 3 days.

[0011] 4. Preparation of inoculum: add the carrier to the fermented broth of the prepared strain and dry it by spray drying technology to prepare the inoculum of Alteromonas colwelliana A321.

[0012] Among them, the medium used in Alteromonas colwelliana A321 in steps 1 to 3 is Enteromorpha polysaccharide medium: ...

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Abstract

The invention relates to a method for preparing a biological fertilizer by utilizing Alteromonas colwelliana A321 to ferment enteromorpha. The enteromorpha biological organic fertilizer is prepared through the processes of slant culture, seed culture, strain culture, preparation of microbial agents, enteromorpha fermentation and preparation. The Alteromonas colwelliana A321 provided by the invention is an autonomously directional induction screened strain and is preserved in CCTCC (China Center for Type Culture Collection) in Wuhan University, with the preservation number of CCTCC NO: M2012132. Characteristic polyose, proteins and cellulose in the intercellular substance and cell walls of the enteromorpha can be efficiently degraded by the microbial agents so as to fully release nutrient elements in the enteromorpha and increase the fermentation speed of the enteromorpha.

Description

technical field [0001] Biotechnology technical background [0002] Enteromorpha is rich in nutrients. The dry basis contains 9-14% protein, 32-36% ash, 6.7-9% water, 2% fat, and the rest is carbohydrates, about 40%. In particular, Enteromorpha contains seaweed polysaccharides, highly unsaturated fatty acids, alginic acid, and natural growth-regulating substances unique to marine organisms. It is a plant widely distributed in the ocean and has a huge reserve. Especially due to frequent outbreaks of green tide Enteromorpha in recent years, a large amount of Enteromorpha is salvaged every year and cannot be effectively treated. Some Enteromorpha accumulates on the shore all the year round, which not only wastes resources, but also pollutes the surrounding environment, which is not conducive to tourism. , fisheries and other industries. In order to make full use of Enteromorpha resources and solve the harm of Enteromorpha to the environment, it is necessary to ferment Enteromo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C05F11/08C12R1/01
Inventor 王鹏王进
Owner OCEAN UNIV OF CHINA
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