Reagent for measurement of leukocytes and hemoglobin concentration in blood

a technology for hemoglobin concentration and leukocyte count, which is applied in the direction of instruments, chemical methods analysis, material analysis, etc., can solve the problems of insufficient stabilizing effect of these stabilizers, affecting the measurement accuracy of leukocyte count, and affecting the quality of blood samples, etc., to achieve the effect of measuring the hemoglobin concentration stably and measuring the leukocyte coun
USRE38131E1Inactive Publication Date: 2003-06-03SYSMEX CORP
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Patent Information

Authority / Receiving Office
US · United States
Patent Type
Patents(United States)
Current Assignee / Owner
SYSMEX CORP
Publication Date
2003-06-03
Estimated Expiration
Not applicable · inactive patent

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Abstract

A reagent for measurement of leukocytes and hemoglobin concentration in the blood includes a cationic surfactant in an amount sufficient to lyse erythrocytes and denature hemoglobin, at least one of the following hemoglobin stabilizers:(a) sulfosalicylic acid, or its salt, in an amount effective for promoting the conversion of hemoglobin into methemoglobin,(b) 0.2 to 10.0 g / L of a water-soluble chelating agent having a nitrogen atom and a carboxyl group, and(c) piperazine, or its salt, in an amount effective for promoting the conversion of hemoglobin into methemoglobin, anda buffer for maintaining pH at 4 to 6.
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Description

BACKGROUND OF THE INVENTIONThis invention relates to a reagent for measurement of leukocytes and hemoglobin concentration in a blood sample.Measuring the leukocyte count and hemoglobin concentration in the blood is very important for the clinical diagnosis of leukemia, anemia, etc. or for monitoring the clinical course in patients.Currently, the leukocyte count and hemoglobin concentration can be measured in a short time by an automatic blood analyzer, and such an analyzer is in widespread use.Automatic blood analyzers can be roughly classified into an optical detection system for detecting light scattering or fluorescence, and an electric resistance detection system for detecting a change in impedance occurring when particles pass through an aperture. The latter system is superior in terms of ease of use. According to the electric resistance detection system, the measurement of the leukocyte count is performed by adding a hemolytic agent to a blood sample to lyse erythrocytes and p...

Claims

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