Method and related kit for detecting c-MET/CEP7 gene status based on rare cells

A rare cell and kit technology, applied in the field of medical diagnostics, can solve the problems of inconsistent c-MET expression, dynamic changes in molecular information, and difficulty in specimen collection.
CN105087775AInactive Publication Date: 2015-11-25北京莱尔生物医药科技有限公司
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
北京莱尔生物医药科技有限公司
Publication Date
2015-11-25
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention relates to a method and a related kit for detecting c-MET / CEP7 gene status based on rare cells, wherein the method comprises the following steps: 1. acquiring a blood sample or a body fluid sample, wherein the sample includes a mixed cell population of circulating tumor cells or other rare cells and white blood cells; 2. diluting the sample by virtue of a buffer solution, removing plasma and recovering CTC or other rare cells; 3. cracking by virtue of a red blood cell lysis buffer, removing red blood cells and recovering CTC or other rare cells; 4. recovering cells with magnetic particles coated with related anti-human leucocyte antigens and antibodies, uniformly mixing and breeding, and fully combining so as to form a magnetic particle-white blood cell mixture; 5. isolating the magnetic particle-white blood cell mixture from other cells so as to obtain a mixed cell population containing CTC or other rare cells and a few of white blood cells; and 6. by virtue of a method combining immunofluorescence cytochemistry and fluorescence in-situ hybridization, determining the status of c-MET / CEP7, and simultaneously identifying CTC or other rare cells.
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Description

technical field

[0001] The present invention relates to medical diagnostics, in particular to the detection of tumor cells. More specifically, the present invention relates to diagnostic methods for detecting cancer and evaluating treatment options for patients. Background technique

[0002] Circulating tumor cells (Circulating Tumor Cell, CTC) refer to the tumor cells that enter the blood circulation, which can fall off into the blood from the primary tumor or metastases, and may also enter the blood before forming solid tumor lesions. Tumor cells invade the circulatory system, and most of them die within a short period of time due to the body’s immune recognition, mechanical killing, and self-apoptosis, and only a few survive and plant in distant or primary tissues and organs, and further develop into metastases. The most direct factor leading to tumor metastasis and recurrence.

[0003] CTC is a "liquid biopsy" sample that can represent the primary tumor. Peripheral blo...

Claims

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