Method and relevant kit for detecting ALK gene status based on rare cells

A rare cell and kit technology, applied in the field of medical diagnostics, can solve the problems of lack of comprehensive and holistic evaluation of histological detection, inability to provide real-time monitoring information, and dynamic changes in molecular information.
CN105087776AInactive Publication Date: 2015-11-25北京莱尔生物医药科技有限公司
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
北京莱尔生物医药科技有限公司
Publication Date
2015-11-25
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention relates to a method and a relevant kit for detecting ALK gene status based on rare cells. The method comprises the following steps: step 1, taking a blood sample or a body fluid sample, wherein the sample contains a mixed cell population comprising circulating tumor cells (CTCs) or other rare cells, and white blood cells; step 2, diluting the sample by adopting a buffer solution, so as to remove plasma and recover the CTCs or the other rare cells; step 3, splitting by adopting red blood cell splitting solution, so as to remove red blood cells and recover the CTCs or the other rare cells; step 4, uniformly mixing magnetic particles coated by relevant human-leukocyte-resisting antigen-antibodies and the recovered cells obtained in the step 3 for incubation, and mixing to form a magnetic particle-white blood cell mixture; step 5, separating the magnetic particle-white blood cell mixture from other cells through centrifugation, and thus obtaining the mixed cell population containing CTCs or other rare cells, and small quantity of white blood cells; and step 6, determining the ALK gene rearrangement state of CTCs or other rare cells by adopting a method of combining the immunofluorescence cytochemistry and fluorescence in situ hybridization.
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Description

technical field

[0001] The present invention relates to medical diagnostics, in particular to the detection of tumor cells. More specifically, the present invention relates to diagnostic methods for detecting cancer and evaluating treatment options for patients. Background technique

[0002] Circulating tumor cells (Circulating Tumor Cell, CTC) refer to the tumor cells that enter the blood circulation, which can fall off into the blood from the primary tumor or metastases, and may also enter the blood before forming solid tumor lesions. Tumor cells invade the circulatory system, and most of them die within a short period of time due to the body’s immune recognition, mechanical killing, and self-apoptosis, and only a few survive and plant in distant or primary tissues and organs, and further develop into metastases. The most direct factor leading to tumor metastasis and recurrence.

[0003] CTC is a "liquid biopsy" sample that can represent the primary tumor. Peripheral blo...

Claims

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