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An immunochemical staining kit for cervical cancer auxiliary diagnosis

A technology of concentration and working solution, applied in the field of liquid-based cytology immunocytochemistry, can solve the problems of false positives, false negatives, missed diagnosis of high-risk patients, etc., and achieve the effect of easy storage, fast sealing and saving operation time.

Active Publication Date: 2022-04-15
肖国林
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, limited by the experience of pathologists, there are certain false positives and false negatives, resulting in the missed diagnosis of some high-risk patients.

Method used

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  • An immunochemical staining kit for cervical cancer auxiliary diagnosis
  • An immunochemical staining kit for cervical cancer auxiliary diagnosis
  • An immunochemical staining kit for cervical cancer auxiliary diagnosis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0075] Example 1 Provides an immunochemical staining kit for auxiliary diagnosis of cervical cancer, including ready-to-use mixed primary antibody working solution (1 bottle), ready-to-use mixed secondary antibody working solution (1 bottle), rapid protein-free blocking solution ( 1 bottle), stable horseradish peroxidase chromogenic reagent (3 bottles), ready-to-use alkaline phosphatase chromogenic reagent (1 bottle), aqueous mounting medium (1 bottle).

[0076] The ready-to-use mixed primary antibody working solution includes PBS buffer solution with a pH value of 7.4, p16 INK4a Monoclonal antibody, Ki-67 monoclonal antibody, bovine serum albumin, trehalose, betaine, β-cyclodextrin, sodium chloride, Tween-20, KATHON; wherein, the p16 INK4aThe antibody concentration is 0.2mg / L; the Ki-67 antibody concentration is 0.3mg / L; the bovine serum albumin concentration is 0.5g / L; the trehalose concentration is 1.7g / L; the betaine concentration It is 3.6g / L; the β-cyclodextrin concen...

Embodiment 2

[0089] Example 2 Provides an immunochemical staining kit for auxiliary diagnosis of cervical cancer, including ready-to-use mixed primary antibody working solution (1 bottle), ready-to-use mixed secondary antibody working solution (1 bottle), rapid protein-free blocking solution ( 1 bottle), stable horseradish peroxidase chromogenic reagent (3 bottles), ready-to-use alkaline phosphatase chromogenic reagent (1 bottle), aqueous mounting medium (1 bottle).

[0090] The ready-to-use mixed primary antibody working solution includes PBS buffer solution with a pH value of 7.2, p16 INK4a Monoclonal antibody, Ki-67 monoclonal antibody, bovine serum albumin, trehalose, betaine, β-cyclodextrin, sodium chloride, Tween-20, KATHON; wherein, the p16 INK4a The antibody concentration is 0.3mg / L; the Ki-67 antibody concentration is 0.3mg / L; the bovine serum albumin concentration is 0.4g / L; the trehalose concentration is 2g / L; the betaine concentration is 2g / L; the concentration of β-cyclodex...

Embodiment 3

[0103] Example 3 Provides an immunochemical staining kit for auxiliary diagnosis of cervical cancer, including ready-to-use mixed primary antibody working solution (1 bottle), ready-to-use mixed secondary antibody working solution (1 bottle), rapid protein-free blocking solution ( 1 bottle), stable horseradish peroxidase chromogenic reagent (3 bottles), ready-to-use alkaline phosphatase chromogenic reagent (1 bottle), aqueous mounting medium (1 bottle).

[0104] The ready-to-use mixed primary antibody working solution includes PBS buffer solution with a pH value of 7.7, p16 INK4a Monoclonal antibody, Ki-67 monoclonal antibody, bovine serum albumin, trehalose, betaine, β-cyclodextrin, sodium chloride, Tween-20, KATHON; wherein, the p16 INK4a The antibody concentration is 1.2mg / L; the Ki-67 antibody concentration is 1.1mg / L; the bovine serum albumin concentration is 2.2g / L; the trehalose concentration is 7g / L; the betaine concentration is 4g / L; the concentration of β-cyclodex...

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Abstract

The invention relates to the field of liquid-based cytology immunocytochemistry, in particular to an immunohistochemistry kit for auxiliary diagnosis of cervical cancer. The present invention provides a ready-to-use antibody working solution, including antibody reagents, bovine serum albumin, non-reducing sugars, betaine, cyclodextrin, neutral inorganic salts, surfactants, and preservatives; wherein the The antibody reagent is reagent A or reagent B; the reagent A is p16 INK4a The PBS buffer solution of monoclonal antibody and Ki-67 monoclonal antibody; The reagent B is the PBS buffer solution of the goat anti-mouse secondary antibody labeled with horseradish peroxidase and the goat anti-rabbit secondary antibody labeled with alkaline phosphatase; The pH value of the PBS buffer solution is 7.2-7.8.

Description

technical field [0001] The invention relates to the field of liquid-based cytology immunocytochemistry, in particular to an immunochemical staining kit for auxiliary diagnosis of cervical cancer. Background technique [0002] Cervical cancer is one of the most common malignant tumors in gynecology. Its incidence is second only to breast cancer and tends to be younger, seriously endangering women's health. However, cervical cancer is the only clear cause, and it is expected to become the first tumor to be conquered by humans. Therefore, prevention and early screening are of great significance to reduce the incidence and mortality of cervical cancer. [0003] At present, the main methods of cervical cancer diagnosis are: cervical exfoliation cytology examination, human papillomavirus (HPV) detection, colposcopy, pathological biopsy, etc.; the three-stage procedure for cervical cancer diagnosis recommended by the Chinese Medical Association is cytology, colposcopy, etc. , Cer...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/52G01N33/574G01N33/535G01N33/577G01N33/58
Inventor 肖国林
Owner 肖国林
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