Analytical method of components of fatty acid contained in listeria cells

Abstract

The invention relates to an analytical method of components of fatty acids contained in listeria cells, in particular to a method for carrying out bacteria classification by adopting a cell chemical analysis method, belonging to the technical field of biological engineering. The method mainly comprises the following steps of: rejuvenating listeria; respectively separating and purifying the listeria by using an OXA agar plate and a trypticase soy yeast extract agar plate; culturing the listeria of a single typical colony, and preparing into a bacterial suspension; carrying out inactivation processing by using formaldehyde; averagely filling the bacterial suspension processed by the formaldehyde into centrifuge tubes for washing; carrying out methyl esterification by using a mixed solution of hydrochloric acid and the formaldehyde; and carrying out GC-MS (Gas Chromatograph-Mass Spectrometer) analysis on the prepared fatty acids. The method can not only break through the limit of the traditional bacterial taxonomy and reduce the errors brought about by anthropic factors on the traditional morphological taxonomy, but also provide a strong and powerful tool for the classification and the identification of new strains and virus types; and in addition, the method can be used for identifying the listeria from other bacteria and foods.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, in particular to a method for classifying bacteria by adopting a cytochemical analysis method. Background technique [0002] The early bacteriological classification mainly adopted the traditional bacteriological classification method based on morphological characteristics and physiological and biochemical characteristics. Until now, morphological characteristics and physiological and biochemical characteristics are still the cornerstone of bacterial taxonomy. However, because the surface characteristics of bacteria are often affected and limited by many human factors, especially the differences in people's subjective judgments, it is difficult to reflect the natural kinship of bacteria, which reflects the fact that bacteria are only based on morphological characteristics and physiological and biochemical characteristics. limitations of traditional taxonomies. Therefore, Colwee1 proposed ...

AI Technical Summary

Problems solved by technology

This kind of method is simple and easy to implement, with high resolution, and plays an increasingly important role in the study of bacterial taxonomy. However, the method of molecular classification is more complicated than the method of chemical classification, with high requirements and higher requirements for instrument operation accuracy.

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