Separation system for CTCs (circulating tumor cells)

A tumor cell and separation system technology, applied in biochemical instruments, biochemical equipment and methods, enzymology/microbiology devices, etc., can solve the problems of low purity of circulating tumor cells, prone to false positives, unreleased detection, etc., to achieve Ease of marketing, low cost, low cost effect

Inactive Publication Date: 2014-02-19
SHANGHAI KANGWEI HEALTH TECH CO LTD
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Problems solved by technology

[0008] 1) The purity of captured circulating tumor cells is low, and there is a large number of non-specific adsorption of white blood cells. Specific staining is required to identify circulating tum

Method used

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  • Separation system for CTCs (circulating tumor cells)
  • Separation system for CTCs (circulating tumor cells)

Examples

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Embodiment Construction

[0032] The present invention is described in detail below in conjunction with accompanying drawing:

[0033] Such as figure 1 As shown, the circulating tumor cell separation system of the present invention includes a sample preparation module, a sample injection module, a first microfluidic chip 3, a second microfluidic chip 4, a release module, a collection module and a control module.

[0034] The sample preparation module is used to process the blood into a test sample, and the test sample is 1000 colon cancer tumor cells HCT116 stained red with the cell membrane red fluorescent probe DiI, and then mixed with 1 ml of anticoagulated healthy person's Whole blood or whole blood that has been lysed by red blood cells is mixed, and 1 microliter of 1 mg / ml antibody-single-stranded labeled polynucleotide complex is added at the same time, and the mixture is placed on a shaker to fully incubate for half an hour to obtain samples. Wherein the antibody-single-stranded labeled polynu...

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Abstract

The invention belongs to the field of biological and medical detection, and relates to a system for separating CTCs (circulating tumor cells) in a blood sample from a mixed cell group in high purity. The separation system for the CTCs comprises a sample preparation module, a sample injection module, a first micro-fluidic chip, a release module and a collection module, wherein the sample preparation module is used for processing blood into a detection sample; the sample injection module is used for injecting the detection sample into the first micro-fluidic chip; the first micro-fluidic chip is used for extracting the CTCs in the detection sample; the release module is used for separating the CTCs from the first micro-fluidic chip and obtaining a collection sample; and the collection module is used for collecting the collection sample. According to the technical scheme, compared with the prior art, the separation system for the CTCs is simple, easy to implement, lower in cost, simple to operate and high in automation degree.

Description

technical field [0001] The invention belongs to the field of biological and medical detection, and relates to a system for separating circulating tumor cells in blood samples with high purity from mixed cell populations. Background technique [0002] Rare cells refer to cells that are rare in biological samples but have important biological functions or clinical detection significance, such as circulating tumor cells in human peripheral blood, circulating endothelial cells, cells infected by viruses, and fetuses in peripheral blood of pregnant women. cells, and tumor stem cells in solid tumors. Due to the extremely rare number of rare cells, it is usually impossible to directly detect them under the interference of a large number of background cells. Therefore, it is necessary to develop efficient and rapid methods and equipment for separating rare cells from complex biological samples. [0003] Circulating tumor cells (CTCs) in human peripheral blood are a representative t...

Claims

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Application Information

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IPC IPC(8): C12M1/00
CPCC12M23/16C12M33/10
Inventor 李小卫
Owner SHANGHAI KANGWEI HEALTH TECH CO LTD
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