Separation system for CTCs (circulating tumor cells)

Abstract

The invention belongs to the field of biological and medical detection, and relates to a system for separating CTCs (circulating tumor cells) in a blood sample from a mixed cell group in high purity. The separation system for the CTCs comprises a sample preparation module, a sample injection module, a first micro-fluidic chip, a release module and a collection module, wherein the sample preparation module is used for processing blood into a detection sample; the sample injection module is used for injecting the detection sample into the first micro-fluidic chip; the first micro-fluidic chip is used for extracting the CTCs in the detection sample; the release module is used for separating the CTCs from the first micro-fluidic chip and obtaining a collection sample; and the collection module is used for collecting the collection sample. According to the technical scheme, compared with the prior art, the separation system for the CTCs is simple, easy to implement, lower in cost, simple to operate and high in automation degree.

Description

technical field [0001] The invention belongs to the field of biological and medical detection, and relates to a system for separating circulating tumor cells in blood samples with high purity from mixed cell populations. Background technique [0002] Rare cells refer to cells that are rare in biological samples but have important biological functions or clinical detection significance, such as circulating tumor cells in human peripheral blood, circulating endothelial cells, cells infected by viruses, and fetuses in peripheral blood of pregnant women. cells, and tumor stem cells in solid tumors. Due to the extremely rare number of rare cells, it is usually impossible to directly detect them under the interference of a large number of background cells. Therefore, it is necessary to develop efficient and rapid methods and equipment for separating rare cells from complex biological samples. [0003] Circulating tumor cells (CTCs) in human peripheral blood are a representative t...

AI Technical Summary

Problems solved by technology

[0008] 1) The purity of captured circulating tumor cells is low, and there is a large number of non-specific adsorption of white blood cells. Specific staining is required to identify circulating tumor cells, which is time-consuming and prone to false positives;

[0009] 2) The captured circulating tumor cells are often fixed on the surface of the chip and cannot be released for subsequent detection

Images