Adipose-derived stem cell isolated culture method

A technology for the separation and cultivation of adipose stem cells, applied in the field of adipose stem cell separation and cultivation, can solve the problems of small number of cells, bone pain, and unsatisfactory adipose stem cell culture effects, and achieve the effect of reducing production costs and improving the efficiency of adipose stem cell separation and cultivation

Inactive Publication Date: 2017-05-31
GUIZHOU BEIKE FACTORR BIOTECH CO LTD
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Problems solved by technology

For clinical research, the application of stem cells in clinical practice is currently mainly to isolate and purify hematopoietic stem cells from the bone marrow system, but during the process of obtaining bone marrow stem cells, bone penetration is extremely painful, and the number of cells obta

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  • Adipose-derived stem cell isolated culture method
  • Adipose-derived stem cell isolated culture method
  • Adipose-derived stem cell isolated culture method

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Embodiment Construction

[0024] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only some of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

[0025] The invention provides a method for isolating and culturing adipose stem cells, which improves the culture efficiency of adipose stem cells by establishing a complete set of adipose stem cell separation and culturing system, comprising the following steps.

[0026] S1, wash the adipose tissue, remove blood cells, add 50ml adipose tissue and 100ml sodium chloride injection to the T175 culture flask whose outer wall has been wiped with 75% al...

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Abstract

The invention relates to the technical field of adipose-derived stem cell isolated culture, and discloses a dental pulp stem cell isolated culture method. The dental pulp stem cell isolated culture method comprises the following steps: washing adipose tissues, digesting the adipose tissues by using collagenase Type I, separating adipose stromal vascular fractions, then carrying out scavenging-precipitation, planting and culturing obtained primary cells to obtain the primary cells in the end, and carrying out primary cell passage. The dental pulp stem cell isolated culture method has the beneficial effects that by standardizing the dental pulp stem cell isolated culture method and various parameters in concrete operations, the adipose-derived stem cell isolated culture efficiency is improved, the output rate is increased, and the production cost is reduced.

Description

technical field [0001] The invention relates to the field of separation and culture of adipose stem cells, in particular to a method for separation and culture of adipose stem cells. Background technique [0002] The stem cell storage business has developed from simple umbilical cord blood storage to the current storage of stem cells from various sources such as umbilical cord, placenta, and teeth, and its importance and necessity have been recognized by more and more people. For clinical research, the application of stem cells in clinical practice is currently mainly to isolate and purify hematopoietic stem cells from the bone marrow system, but during the process of obtaining bone marrow stem cells, bone penetration is extremely painful, and the number of cells obtained is small. Adipose stem cells have the advantages of wide sources, easy acquisition, less pain for patients, and sufficient cell volume. However, there is currently a lack of a complete system for the cultiv...

Claims

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Application Information

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IPC IPC(8): C12N5/0775
Inventor 张凤宁
Owner GUIZHOU BEIKE FACTORR BIOTECH CO LTD
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