Adipose-derived stem cell isolated culture method

Abstract

The invention relates to the technical field of adipose-derived stem cell isolated culture, and discloses a dental pulp stem cell isolated culture method. The dental pulp stem cell isolated culture method comprises the following steps: washing adipose tissues, digesting the adipose tissues by using collagenase Type I, separating adipose stromal vascular fractions, then carrying out scavenging-precipitation, planting and culturing obtained primary cells to obtain the primary cells in the end, and carrying out primary cell passage. The dental pulp stem cell isolated culture method has the beneficial effects that by standardizing the dental pulp stem cell isolated culture method and various parameters in concrete operations, the adipose-derived stem cell isolated culture efficiency is improved, the output rate is increased, and the production cost is reduced.

Description

technical field [0001] The invention relates to the field of separation and culture of adipose stem cells, in particular to a method for separation and culture of adipose stem cells. Background technique [0002] The stem cell storage business has developed from simple umbilical cord blood storage to the current storage of stem cells from various sources such as umbilical cord, placenta, and teeth, and its importance and necessity have been recognized by more and more people. For clinical research, the application of stem cells in clinical practice is currently mainly to isolate and purify hematopoietic stem cells from the bone marrow system, but during the process of obtaining bone marrow stem cells, bone penetration is extremely painful, and the number of cells obtained is small. Adipose stem cells have the advantages of wide sources, easy acquisition, less pain for patients, and sufficient cell volume. However, there is currently a lack of a complete system for the cultiv...

AI Technical Summary

Problems solved by technology

For clinical research, the application of stem cells in clinical practice is currently mainly to isolate and purify hematopoietic stem cells from the bone marrow system, but during the process of obtaining bone marrow stem cells, bone penetration is extremely painful, and the number of cells obtained is small

Adipose stem cells have the advantages of wide sources, easy acquisition, less pain for patients, and sufficient cell volume. However, at present, there is a lack of a complete system for the cultivation of adipose stem cells, and the culture effect of adipose stem cells is not ideal.

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