Method for sorting human marrow mesenchymal stemcell by monoclonal antibody ZUF10 immunomagnetic bead

An immunomagnetic bead sorting and bone marrow mesenchymal technology, which is applied in the direction of bone/connective tissue cells, animal cells, vertebrate cells, etc., can solve the problem of complex components of CD45-GlycophorinA- cell populations and the difficulty of direct sorting by immunolabeling Ideal labeling of human bone marrow MSCs, difficulties in the practical application of MSCs isolation and purification, etc., to achieve the effect of improving time-consuming, shortening time, and improving efficiency
CN100562569CInactive Publication Date: 2009-11-25ZHEJIANG UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
ZHEJIANG UNIV
Publication Date
2009-11-25
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention provides the method of separating and choosing the dry cell in the marrow using the single resisting ZUF10 immune magnetoelectric bead. The medullary suspending liquid is separated to gain the single nuclear cell. Using the single clonal antibody ZUF10 for the sign, the full dry cell in the human marrow with the ZUF10 masculine antigen by the indirect immune magnetoelectric bead choosing system is gained. The full dry cell in the human body has the good developing active capability and the good proliferating potential. The ZUF10 antigen during the process of the cell bearing is steady and can be directionally induced and separated to the lipocyte, the bone cell, the nerve cell. The invention provides the perfect method of choosing and sublimating the full dry cell in the human marrow and advances the purity of the full dry cell between the original era and the efficiency of separating and culturing. The invention is propitious to the research and the extending in the clinic checking and the application of the biology characteristic of the full dry cell.
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Description

Technical field:

[0001] The invention belongs to the field of biological technology, and relates to a new method for the separation, purification and in vitro culture of primary cells of human bone marrow mesenchymal stem cells. The method uses a newly prepared anti-human bone marrow mesenchymal stem cell monoclonal antibody, which The sorting method effectively separates and purifies mesenchymal stem cells from human bone marrow mononuclear cells, cultures and expands them in vitro, and induces multilineage differentiation. Background technique:

[0002] Human bone marrow mesenchymal stem cells (Mesenchymal stem cells, MSCs) are stem cells with high self-renewal and multi-lineage differentiation potential existing in the bone marrow, and are ideal seed cells for tissue engineering and regenerative medicine. There are a large number of basic and clinical research reports on bone marrow MSCs at home and abroad, which have broad prospects in the clinical application of stem ce...

Claims

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