Primary isolation culture method of condylar cartilage stem cells

A culture method, a technology of separation and culture, applied in the field of stem cell culture, can solve the problem that the etiology of condylar osteochondroma is not yet clear, and achieve the effect of simple operation and high efficiency of separation and culture

Pending Publication Date: 2021-03-09
SICHUAN UNIV
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Problems solved by technology

[0003] The etiology of condylar osteochondroma is unknown

Method used

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  • Primary isolation culture method of condylar cartilage stem cells
  • Primary isolation culture method of condylar cartilage stem cells
  • Primary isolation culture method of condylar cartilage stem cells

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Embodiment 1

[0045] Embodiment 1 The primary cell extraction of human condylar cartilage stem cells of the present invention

[0046] 1. Primary isolation and culture method

[0047] For the isolation and extraction of human condylar osteochondroma condylar cartilage stem cells:

[0048] 1. Fresh human condylar osteochondroma tissue (including condylar cartilage layer) was placed in DPBS containing 1% penicillin / streptomycin

[0049] , store and transport on ice.

[0050] 2. In an ultra-clean bench, carefully peel off the cartilage surface layer of the condylar cartilage under a stereomicroscope using a scalpel, microtissue scissors, and ophthalmic forceps (see figure 1 ), during the operation, it is necessary to keep the tissue in a moist state, place the stripped cartilage surface in a culture dish filled with fresh pre-cooled DPBS, use tissue scissors to cut it into small pieces of about 1*1mm, and use a pipette Aspirate the DPBS containing the tissue fragments with a liquid gun and ...

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Abstract

The invention provides a primary isolation culture method of condylar cartilage stem cells. The primary isolation culture method comprises the following steps of: a, taking a human condylar cartilagesurface layer, cutting into pieces, cleaning, and digesting by using I-type collagenase; b, after digestion is ended, centrifuging, removing supernatant, cleaning, adding a cell culture medium, resuspending, and carrying out adherent culture; and c, changing liquid, carrying out digestion passage, culturing, separating and screening the condylar cartilage stem cells by adopting a flow sorting method, and culturing. The primary isolation culture method provided by the invention is simple and convenient to operate, can be used for effectively separating osteochondral tumor condylar cartilage stem cells from normal human condylar cartilage stem cells and has a good application prospect.

Description

technical field [0001] The invention belongs to the field of stem cell culture, and in particular relates to a method for primary isolation and culture of condylar cartilage stem cells. Background technique [0002] Osteochondroma (OC), also known as osteocartilaginous exotosis, is a common benign bone tumor. Strictly speaking, it is an abnormal growth. The tumor has a cartilage cap and a bone protruding from the side of the bone. organize. The condyle is the most easily affected part of maxillofacial osteochondroma, and the main manifestations of condylar osteochondroma are lumps in the joint area and abnormal joint function, often secondary to asymmetric dental and maxillofacial deformities. [0003] The etiology of condylar osteochondroma is unknown. From histological analysis, the surface of the condyle is covered with fibrocartilage, which can be divided into articular surface zone, proliferative zone, hypertrophic zone and calcified cartilage zone from the surface la...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/0775
CPCC12N5/0662C12N2509/00C12N2509/10
Inventor 毕瑞野祝颂松尹晴樊怡
Owner SICHUAN UNIV
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