Preparation method of rare-earth coordination polymer fluorescence probe and application of rare-earth coordination polymer fluorescence probe in H2O2 and glucose detection

A technology of coordination polymers and fluorescent probes, applied in the field of optical sensing, can solve problems such as rare earth coordination polymer nanoparticles, etc., and achieve high sensitivity and good selectivity.

Inactive Publication Date: 2016-09-21
NANCHANG UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

So far, there has been no report on the self-assembly of rare earth coordination polymer nanoparticles using ATP molecules as ligands and rare earth ions

Method used

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  • Preparation method of rare-earth coordination polymer fluorescence probe and application of rare-earth coordination polymer fluorescence probe in H2O2 and glucose detection
  • Preparation method of rare-earth coordination polymer fluorescence probe and application of rare-earth coordination polymer fluorescence probe in H2O2 and glucose detection
  • Preparation method of rare-earth coordination polymer fluorescence probe and application of rare-earth coordination polymer fluorescence probe in H2O2 and glucose detection

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Embodiment 1

[0020]Preparation of ATP-Ce-Tris: Mix 0.2 mL of 10 mM adenosine triphosphate (ATP) with 1.6 mL of 50 mM pH 7.4 Tris-HCl buffer solution, and then add 1 mL of 8 mM Ce(NO 3 ) 3 ·6H 2 O aqueous solution was slowly stirred at a rate of 300 rpm / min at room temperature for 1 min to form a white precipitate. The white precipitate was centrifuged at 16000 rpm / min for 15 min, and then washed with ultrapure water. After repeated centrifugation and washing 3 times, the obtained precipitate was dispersed in 2 mL of ultrapure water to prepare ATP-Ce-Tris rare earth complex. Polymeric fluorescent probes.

[0021] ATP-Ce-Tris, ATP-Ce, Ce-Tris and Ce(NO 3 ) 3 Fluorescent properties were characterized, and the results were as follows: figure 1 shown. Under the excitation of light with a wavelength of 310 nm, Ce(NO 3 ) 3 The fluorescence emission peak of the aqueous solution is located at 350 nm; when ATP, Tris and Ce 3+ After mixing, the fluorescence emission peak of the obtained ATP...

Embodiment 2

[0023] Rare earth coordination polymer fluorescent probe for detection of H 2 o 2 and glucose

[0024] (1) Detection of H 2 o 2 : Mix 20 μL of 4 mM ATP-Ce-Tris, 40 μL of 50 mM Tris-HCl pH 7.4 and different concentrations of H 2 o 2 Mix, add ultrapure water to bring the total volume of the test solution to 200 μL, react at room temperature for 25 min, and measure the fluorescence of the solution when the excitation wavelength is 310 nm. Figure 5 a is ATP-Ce-Tris pair H 2 o 2 The detected fluorescence spectrum, along with the H 2 o 2 As the concentration increases, the fluorescent Ce in ATP-Ce-Tris 3+ Gradually H 2 o 2 Oxidized to non-fluorescent Ce 4+ , causing the fluorescence intensity of ATP-Ce-Tris to gradually decrease, and the rate of change of fluorescence intensity of ATP-Ce-Tris (1-F / F 0 ) with H 2 o 2 The logarithm of the concentration has a good linear relationship in the range of 1 nM-80 μM ( Figure 5 b), with a detection limit of 0.6 nM, enabling ...

Embodiment 3

[0027] Examined the ATP-Ce-Tris effect on H 2 o 2 and selectivity for glucose detection ( Figure 7 ). Depend on Figure 7 e Visible, ATP-Ce-Tris for H 2 o 2 During detection, interfering ions include K + , Na + , Ca 2+ , Mg 2+ , PO 4 3- , SO 4 2- , CO 3 2- , Cl - , Ac - etc. No response. Depend on Figure 7 It can be seen from f that when ATP-Ce-Tris detects glucose, the interfering ions include cations (K + , Na + , Ca 2+ , Mg 2+ ) and carbohydrates (lactose (lac), sucrose (suc), fructose (fru), mannose (man)) etc. did not respond. The above results show that the rare earth coordination polymer fluorescent probe ATP-Ce-Tris constructed by the present invention has a positive effect on H 2 o 2 and glucose detection have good selectivity.

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Abstract

The invention discloses a preparation method of a rare-earth coordination polymer fluorescence probe and an application of the rare-earth coordination polymer fluorescence probe in H2O2 and glucose detection and belongs to the technical field of optical sensing. The preparation method comprises steps as follows: triphosadenine is mixed with a Tris-HCl buffer solution containing Ce<3+>, and the rare-earth coordination polymer fluorescence probe ATP-Ce-Tris is prepared; when H2O2 is present in the solution, Ce<3+> in the ATP-Ce-Tris is oxidized into Ce<4+> by H2O2 to result in fluorescence quenching of the ATP-Ce-Tris, and accordingly, detection of H2O2 is realized; simple and sensitive detection of glucose is further realized through fluorescence quenching of the ATP-Ce-Tris due to H2O2 which is produced through catalytic oxidation of glucose by glucose oxidases.

Description

Technical field [0001] The present invention involves a preparation method and H in the preparation method of a rare earth supporting polymer fluorescent probe and its H 2 O 2 And glucose detection application belongs to the field of optical sensing technology. Background technique [0002] Due to its unique electronic structure, rare earth element has excellent photoelectric properties, especially its prominent catalytic properties and free radical clearance capabilities.Two prices (CE 3+ , CE 4+ The existence of), and the reverse conversion between the two prices, make the rare earth compounds a good antioxidant with good performance.Among them, the antioxidant effect of nano oxide and the biological antioxidant mechanism through the doped SM 3+ The verification, the research results show that CE 3+ / CE 4+ The oxidation reactions between them are the main factor of this unique antioxidant.Based on this special performance of rare earth compounds, Maryna Ornatska et al. Reported...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08G83/00C09K11/06G01N21/64
CPCC08G83/00C09K11/06C09K2211/182G01N21/643
Inventor 邱建丁曾慧慧梁汝萍
Owner NANCHANG UNIV
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