Nervous tissue engineering fibrous membrane and preparation method

A technology of engineering fiber and nerve tissue, applied in non-woven fabrics, textiles, papermaking, medical science, etc., can solve problems such as insufficient spatial resolution and unsatisfactory results, and achieve convenient manipulation, high conductivity, and safety high effect

Inactive Publication Date: 2012-06-20
WUXI ZHONGKE GUANGYUAN BIOMATERIALS
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Problems solved by technology

[0003] However, there are few studies on the electrical and morphological synergistic effects of guiding and expanding neurons. Some studies have made polypyrrole (PPY) microchannels on sheets covered with indium tin oxide (ITO) by etching techniques, and studied How hippocampal cells of embryonic rats respond to combined electrical and morphological signals. The

Method used

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Embodiment Construction

[0014] In order to deepen the understanding of the present invention, the present invention will be further described below in conjunction with the examples.

[0015] A preparation method of nerve tissue engineering fiber membrane, the steps are as follows:

[0016] (1) Preparation of electrospinning solution: Polylactic acid (PLA) and polycaprolactone (PCL) were respectively dissolved in dichloromethane to prepare polymer solutions with a mass percent concentration of 1% and 10% W / V.

[0017] (2) Preparation of polymer fiber membrane: the polymer solution is injected into a 5ml syringe, connected to No. 5 stainless steel pillow (diameter 3mm), the electrospinning condition is a voltage of 15 kV, a flow rate of 1.0ml / h, and a receiving distance of 20cm. The neatly aligned nanofibers were collected with a stainless steel frame (with a 2 cm x 5 cm cavity in the middle).

[0018] (3) Preparation of core-sheath fibers: soak the polymer fiber membrane in 0.05M pyrrole aqueous solu...

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Abstract

The invention discloses a nervous tissue engineering fibrous membrane and a preparation method. The method comprises the following steps of: dissolving a high molecular material into water or an organic solvent to prepare electrostatic spinning solution; injecting the high molecular solution into an injector; performing electro-spinning to form nanofibers, which are arranged tidily, to prepare a high molecular fibrous membrane; and immersing the high molecular fibrous membrane into pyrrole aqueous solution to form core-sheath fibers. In the preparation method of the nervous tissue engineering fibrous membrane provided by the invention, the fibrous membrane is constituted by conductive core-shell nanofibers; the core is high molecule which has excellent material biocompatibility; the nanofibers are arranged tidily by controlling the preparation conditions; the shell-layer material is polypyrrole; the biocompatibility is good; the prepared core-sheath fiber can provide a unique platform to grow and extend neurites; and the process is simple, easy to operate and low in cost and has broad application prospect in the field of nervous tissue engineering.

Description

Technical field: [0001] The invention relates to a nerve tissue engineering fiber membrane, in particular to a fiber membrane with synergistic effects of nerve morphology and electrical stimulation and a preparation method thereof. Background technique: [0002] The ultimate goal of neural tissue engineering is to spontaneously exhibit biochemical, morphological, and electrical properties of neural tissue. However, in most previous studies, the effects of morphology and electrical stimulation, or biochemical properties on synapses, have been examined separately; it has been demonstrated that dorsal root nerves can be controlled using aligned electrospun fibers as templates. The direction of synapse growth in DRG synapses, and even guide axon growth and glial cell migration; Recently, it has been reported that embryonic stem cells can be differentiated into neurons on poly(E-caprolactone) fibers (PCL), and with Compared with random fibers, neatly aligned fibers can improve t...

Claims

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Application Information

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IPC IPC(8): A61L27/34A61L27/16A61L27/18D04H1/728
Inventor 韩志超许杉杉
Owner WUXI ZHONGKE GUANGYUAN BIOMATERIALS
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