Coaxial electrostatic spinning fibrous scaffold and preparation method thereof

A technology of coaxial electrospinning and fiber scaffolding, which is applied in spinning solution preparation, fiber processing, filament/thread forming, etc., can solve the problems of few BMSCs and weak differentiation ability, and achieve effective and rapid repair and promotion of cartilage The effect of direction differentiation, safety, non-toxicity and biocompatibility

Active Publication Date: 2014-07-02
PEKING UNIV THIRD HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] In order to solve the problem that the prior art coaxial electrospun fiber scaffolds recruit less BMSCs in the cartilage defect area and t

Method used

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  • Coaxial electrostatic spinning fibrous scaffold and preparation method thereof
  • Coaxial electrostatic spinning fibrous scaffold and preparation method thereof
  • Coaxial electrostatic spinning fibrous scaffold and preparation method thereof

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Experimental program
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Effect test

Embodiment 1

[0049] The embodiment of the present invention provides a coaxial electrospun fiber scaffold 5, the fiber scaffold includes an aliphatic polyester shell layer 51 and a polyvinylpyrrolidone core layer 52, the surface of the aliphatic polyester shell layer 51 is coupled with bone marrow Mesenchymal stem cell affinity peptide, the polyvinylpyrrolidone core layer 52 is loaded with recombinant human transforming growth factor-β1.

[0050] An embodiment of the present invention provides a coaxial electrospun fiber scaffold 5, which includes an aliphatic polyester shell 51 coupled with a bone marrow mesenchymal stem cell affinity peptide and a polysaccharide loaded with recombinant human transforming growth factor-β1. Vinylpyrrolidone core layer 52 . Through the combination of the aliphatic polyester shell layer 51 and the polyvinylpyrrolidone core layer 52, not only the fiber scaffold has the characteristics of safety, non-toxicity and excellent biocompatibility, but also the fiber ...

Embodiment 2

[0052] The embodiment of the present invention provides a coaxial electrospun fiber scaffold 5, the fiber scaffold includes an aliphatic polyester shell layer 51 and a polyvinylpyrrolidone core layer 52, the surface of the aliphatic polyester shell layer 51 is coupled with bone marrow Mesenchymal stem cell affinity peptide, the polyvinylpyrrolidone core layer 52 is loaded with recombinant human transforming growth factor-β1 and bovine serum albumin.

[0053] Since polyvinylpyrrolidone is a safe and non-toxic water-soluble pharmaceutical intermediate and pharmaceutical adjuvant, it can be miscible or compounded with various substances, making the coaxial electrospun fiber scaffold 5 safe and non-toxic and has excellent biocompatibility ; Because the N-H or O-H bond of polyvinylpyrrolidone can form intermolecular associations with various drugs and / or bioactive factors, and control the release time and action intensity of drugs and / or bioactive factors through the association, P...

Embodiment 3

[0063] as attached figure 1 As shown, the embodiment of the present invention provides a method for preparing a coaxial electrospun fiber scaffold 5, including:

[0064] Step 101: preparing an aliphatic polyester shell solution: dissolving the aliphatic polyester in an organic solvent and stirring until completely dissolved to obtain an aliphatic polyester shell solution.

[0065] Step 102: Prepare a polyvinylpyrrolidone core layer solution containing recombinant human transforming growth factor-β1: dissolve polyvinylpyrrolidone in the same organic solvent, stir until completely dissolved to obtain a polyvinylpyrrolidone core layer solution, and add the polyvinylpyrrolidone core layer solution to the polyvinylpyrrolidone core layer solution Add recombinant human transforming growth factor-β1 into the layer solution, stir until mixed evenly, and obtain polyvinylpyrrolidone core layer solution containing recombinant human transforming growth factor-β1.

[0066] Step 103: In a w...

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Abstract

The invention discloses a coaxial electrostatic spinning fibrous scaffold, belonging to the field of biological tissue engineering. The scaffold comprises an aliphatic polyester shell coupled with bone marrow derived mesenchymal stem cell (BMSC) affinity peptides and a polyvinylpyrrolidone core loading a recombinant human transforming growth factor (TGF)-beta1, not only is safe and non-toxic and has excellent biocompatibility but also can collect more BMSCs and promote chondrogenic differentiation of BMSCs, and then more cartilage tissues are obtained, so that cartilages are effectively and quickly repaired. The invention also discloses a preparation method of the coaxial electrostatic spinning fibrous scaffold. The preparation method comprises the steps of carrying out coaxial electrostatic spinning by using an aliphatic polyester shell solution and a polyvinylpyrrolidone core solution containing the recombinant human TGF-beta1 in the windless environment to prepare a fibrous scaffold; coupling the BMSC affinity peptides on the surface of the fibrous scaffold, thus obtaining a nanoscale coaxial electrostatic spinning fibrous scaffold. The method is simple, is easy to control and has relatively strong practicability.

Description

technical field [0001] The invention relates to the field of biological tissue engineering, in particular to a coaxial electrospinning fiber support and a preparation method thereof. Background technique [0002] Fibrous scaffold is a biological tissue engineering scaffold. Because it has a nanoscale structure similar to natural extracellular matrix, it can mimic the structural characteristics of extracellular matrix. It can support cell growth, guide tissue regeneration, control tissue structure and release biologically active factors. And so on. Since the material and structure of the fibrous scaffold are the main factors affecting its function, the preparation of the fibrous scaffold is very important. Nano-scale fibers prepared by coaxial electrospinning technology not only have high specific surface area and porosity, but also have a structure similar to extracellular matrix, which can be effectively used in tissue engineering to repair cartilage damage. In the proces...

Claims

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Application Information

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IPC IPC(8): A61L27/18A61L27/34A61L27/22A61L27/38A61L27/54D01D5/34D01D1/02
Inventor 敖英芳满振涛邵振兴陈海峰黄洪杰
Owner PEKING UNIV THIRD HOSPITAL
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