Calcium phosphate bone cement with hollow through structure, preparation method and application thereof

Abstract

The invention discloses a calcium phosphate bone cement with a hollow through structure, a preparation method and an application thereof, belongs to the technical field of calcium phosphate bone cement, wherein the calcium phosphate bone cement with a hollow through structure comprises a solid phase and a solidifying liquid, the solid phase comprises calcium phosphate powder and a fiber mixture, the fiber mixture is an absorbable fiber and a fibrin fiber, and the diameter of the absorbable fiber is 100-500mum, the length is 0.5 mm-1.5 mm, preferably, that diameter of the absorbable fiber is 200-400mum. The prepared calcium phosphate bone cement can form three-dimensional connected macropores, promote cell adhesion and proliferation, and improve the mechanical strength of the bone cement.

Description

technical field

[0001] The invention relates to the technical field of calcium phosphate bone cement, in particular to a calcium phosphate bone cement with a hollow through structure, a preparation method and an application thereof. Background technique

[0002] Calcium Phosphate Cement (CPC) was successfully developed by Borwn and Chow in the 1990s. It is a self-curing non-ceramic hydroxyapatite artificial bone material. It is composed of solid phase powder and solidification liquid mixed according to a certain ratio to form an easy-to-shape slurry, which solidifies itself in a short time, and the final hydration product is apatite, which is similar to the inorganic composition and crystal structure of human bone tissue. . CPC has good osteoconductivity and biocompatibility.

[0003] Polylactic-co-glycolic acid (PLGA) is a high ester polymer obtained by the copolymerization of lactide (LA) and glycolide (GA), which has good biocompatibility, complete For a long time, it ...

Examples

Embodiment 1

[0038] Preparation of fibrin fibers:

[0039] Weigh 450 mg of human fibrinogen, dissolve it in 2.25 ml of hexafluoroisopropanol solution, stir magnetically for 30 minutes, add 0.25 ml of normal saline and stir for 10 minutes to obtain an 18% fibrinogen solution;

[0040] The fibrinogen solution is subjected to high-voltage electrospinning, the voltage is set at 15kV, the spinning rate is 1.0 ml / h, the electrospun fibers are received by a metal carrier, and the electrospun fibrin is obtained after 2.5 hours, and the fibers are cut into fibers with a length of 0.5mm protein fibers.

Embodiment 2

[0042] As an embodiment of the calcium phosphate bone cement described in the present invention, the calcium phosphate bone cement described in this embodiment includes solid phase powder and solidification liquid;

[0043] 70 parts by weight of absorbable fiber PLGA and 30 parts by weight of fibrin fiber are uniformly mixed to form a mixed fiber, the length of the absorbable fiber PLGA is 0.5 mm, the diameter is 100 microns, and the length of the fibrin fiber is 0.5 mm. Mix 95% mass fraction of a-calcium phosphate powder with 5% mass fraction of mixed fiber, prepare the bone cement according to the amount of 0.3g liquid phase solidification solution per gram of solid phase; wherein the mass fraction of solidification solution is 4% Na 2 HPO 4 solution.

Embodiment 3

[0045] As an embodiment of the calcium phosphate bone cement described in the present invention, the calcium phosphate bone cement described in this embodiment includes solid phase powder and solidification liquid;

[0046]80 parts by weight of absorbable fiber PLGA and 20 parts by weight of fibrin fiber are uniformly mixed to form a mixed fiber, the length of the absorbable fiber PLGA is 1 mm, the diameter is 250 microns, and the length of the fibrin fiber is 0.5 mm. Mix 85% mass fraction of a-calcium phosphate powder with 15% mass fraction of mixed fiber, prepare the bone cement according to the amount of 0.5g liquid phase solidification solution per gram of solid phase; wherein the mass fraction of solidification liquid is 4% Na 2 HPO 4 solution.

technical field

[0001] The invention relates to the technical field of calcium phosphate bone cement, in particular to a calcium phosphate bone cement with a hollow through structure, a preparation method and an application thereof. Background technique

[0002] Calcium Phosphate Cement (CPC) was successfully developed by Borwn and Chow in the 1990s. It is a self-curing non-ceramic hydroxyapatite artificial bone material. It is composed of solid phase powder and solidification liquid mixed according to a certain ratio to form an easy-to-shape slurry, which solidifies itself in a short time, and the final hydration product is apatite, which is similar to the inorganic composition and crystal structure of human bone tissue. . CPC has good osteoconductivity and biocompatibility.

[0003] Polylactic-co-glycolic acid (PLGA) is a high ester polymer obtained by the copolymerization of lactide (LA) and glycolide (GA), which has good biocompatibility, complete For a long time, it ...

Examples

Embodiment 1

[0038] Preparation of fibrin fibers:

[0039] Weigh 450 mg of human fibrinogen, dissolve it in 2.25 ml of hexafluoroisopropanol solution, stir magnetically for 30 minutes, add 0.25 ml of normal saline and stir for 10 minutes to obtain an 18% fibrinogen solution;

[0040] The fibrinogen solution is subjected to high-voltage electrospinning, the voltage is set at 15kV, the spinning rate is 1.0 ml / h, the electrospun fibers are received by a metal carrier, and the electrospun fibrin is obtained after 2.5 hours, and the fibers are cut into fibers with a length of 0.5mm protein fibers.

Embodiment 2

[0042] As an embodiment of the calcium phosphate bone cement described in the present invention, the calcium phosphate bone cement described in this embodiment includes solid phase powder and solidification liquid;

[0043] 70 parts by weight of absorbable fiber PLGA and 30 parts by weight of fibrin fiber are uniformly mixed to form a mixed fiber, the length of the absorbable fiber PLGA is 0.5 mm, the diameter is 100 microns, and the length of the fibrin fiber is 0.5 mm. Mix 95% mass fraction of a-calcium phosphate powder with 5% mass fraction of mixed fiber, prepare the bone cement according to the amount of 0.3g liquid phase solidification solution per gram of solid phase; wherein the mass fraction of solidification solution is 4% Na 2 HPO 4 solution.

Embodiment 3

[0045] As an embodiment of the calcium phosphate bone cement described in the present invention, the calcium phosphate bone cement described in this embodiment includes solid phase powder and solidification liquid;

[0046]80 parts by weight of absorbable fiber PLGA and 20 parts by weight of fibrin fiber are uniformly mixed to form a mixed fiber, the length of the absorbable fiber PLGA is 1 mm, the diameter is 250 microns, and the length of the fibrin fiber is 0.5 mm. Mix 85% mass fraction of a-calcium phosphate powder with 15% mass fraction of mixed fiber, prepare the bone cement according to the amount of 0.5g liquid phase solidification solution per gram of solid phase; wherein the mass fraction of solidification liquid is 4% Na 2 HPO 4 solution.