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Injectable bone induction repair material and preparation method thereof

A repair material and osteoinductive technology, which can be used in pharmaceutical formulations, prostheses, drug delivery, etc. to achieve the effects of high crystal purity, easy shaping, and convenient operation.

Inactive Publication Date: 2022-01-11
深圳市迈捷生命科学有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to provide an injectable osteoinductive repair material and its preparation method, which uses composite inorganic mineralized particles to solve the pore structure that cannot be achieved by artificial synthetic materials, and at the same time eliminates allogeneic bone antigenicity and ethical issues. Problems in aspects; the injectable osteoinductive repair material of the present invention also has ultra-high crystallinity and crystal purity, and retains the bone trabeculae of natural bone, and its pores are interconnected to facilitate the adhesion and growth of osteoblasts

Method used

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  • Injectable bone induction repair material and preparation method thereof
  • Injectable bone induction repair material and preparation method thereof
  • Injectable bone induction repair material and preparation method thereof

Examples

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Effect test

Embodiment 1

[0040] Example 1: Morphological characteristics and properties of the osteoinductive repair material of the present invention

[0041] A preparation of osteoinductive repair material, comprising the following steps:

[0042]Step S1, prefabricated composite inorganic mineralized particles: select the cancellous bone part of the femoral metaphysis of the limbs of mammals as the biological bone, and cut the biological bone into 2 cm with a linear cutter 3 put the small pieces into a steamer, boil the water for 30 minutes, rinse them with clean water to remove surface impurities, replace the water in the steamer, and repeat the above steaming and rinsing steps 3 times; Add an equal volume of mixed clear water and 40% hydrogen peroxide solution, and continue to boil for 1 hour. After all the small biological bone pieces turn white, remove the white bone pieces and rinse them with clean water, and boil them again with clean water for 1 hour. Take it out and wash it, dry it in an ov...

Embodiment 2

[0048] Example 2: The effect of the osteoinductive repair material of the present invention on cell proliferation and differentiation

[0049] A preparation of osteoinductive repair material, comprising the following steps:

[0050] Step S1, prefabricated composite inorganic mineralized particles: select the cancellous bone part of the femoral metaphysis of the limbs of mammals as the biological bone, and cut the biological bone into 2 cm with a linear cutter 3 put the small pieces into a steamer, boil the water for 30 minutes, rinse them with clean water to remove surface impurities, replace the water in the steamer, and repeat the above steaming and rinsing steps 4 times; Add an equal volume of mixed clear water and 30% hydrogen peroxide solution, and continue to boil for 1 hour. After all the small pieces of biological bone turn white, remove the white bone pieces and rinse them with clean water, and boil them again with clean water for 1 hour. Take it out and wash it, dry...

Embodiment 3

[0057] Example 3: Effect of osteoinductive repair material of the present invention on ectopic osteogenesis

[0058] The osteoinductive repair material prepared in Example 2 was implanted into one side of the leg muscles of rats, and the newly formed hard tissue was analyzed 6 weeks after the implantation, and the dose used was 300 mg. The extracted tissues were stored in formalin solution with a mass fraction of 10% and fixed. After fixing, the tissues were collected and trimmed. According to the conventional paraffin section preparation method, dehydration, embedding, sectioning and other steps were performed, and then conventional HE ( Hematoxylin-Eosin) staining, and HE stained tissue sections were made after sealing.

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Abstract

The invention relates to an injectable bone induction repair material and a preparation method thereof, and belongs to the technical field of biomedical materials. The preparation method specifically comprises the following steps of: S1, performing mineralization treatment on biological bones to obtain mineral substances from the biological bones, and crushing the mineral substances from the biological bones to obtain composite inorganic mineralized particles; S2, performing oscillation adsorption on active factors and the composite inorganic mineralized particles, so that the active factors are stably adsorbed on the surfaces of the composite inorganic mineralized particles through electrostatic attraction to obtain adsorbed composite inorganic mineralized particles; and S3, uniformly mixing the adsorbed composite inorganic mineralized particles with a gel aqueous solution to obtain the bone induction repair material. The bone induction repair material prepared by the preparation method is easy to shape and can be used through injection; and the bone induction repair material has ultrahigh crystallinity and crystallization purity, and pores are communicated with each other, so that cells can be combined with the active factors to promote cell recruitment.

Description

technical field [0001] The invention belongs to the technical field of biomedical materials, and in particular relates to an injectable osteoinductive repair material and a preparation method thereof. Background technique [0002] When the bones of the human body are damaged, the bones need to be repaired. Natural autologous bone is the most ideal donor in terms of osteoconductivity, osteoinductivity and osteogenic ability. However, due to limited sources, when the defect site is large, it cannot provide enough bone source, and it also seriously threatens human health. healthy. [0003] At present, there are many types of repair materials used for clinical bone defects, mainly including artificially synthesized hydroxyapatite, organic bone cement, allogeneic bone, α-tricalcium phosphate, β-tricalcium phosphate, and added collagen, bone, etc. Orthopedic products of active factors such as morphogenetic proteins. [0004] Due to the limitations in the number of clinical appl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/36A61L27/22A61L27/24A61L27/54A61L27/50
CPCA61L27/3608A61L27/365A61L27/3687A61L27/3691A61L27/227A61L27/24A61L27/54A61L27/50A61L2400/06A61L2430/02C08L89/00
Inventor 张建光邓荣霞李秋罗鹏韩苗苗
Owner 深圳市迈捷生命科学有限公司
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