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HMBG (High Mobility Group Box) 1 modified bone tissue engineering bracket material and preparation method thereof

A bone tissue engineering and scaffold material technology, applied in the field of nanofiber bone repair scaffold and its preparation, can solve the problems of difficult scaffold preparation, limited ability to regulate the repair process, unfavorable cell invasion and migration, etc.

Active Publication Date: 2014-07-16
CHONGQING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are still great difficulties in the preparation of ideal bone tissue engineering scaffolds
For example, the nanofibrous scaffold prepared by electrospinning technology is not conducive to the infiltration and migration of cells into the material when the fiber diameter reaches a certain level, and the ability to regulate the repair process is also very limited.

Method used

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  • HMBG (High Mobility Group Box) 1 modified bone tissue engineering bracket material and preparation method thereof
  • HMBG (High Mobility Group Box) 1 modified bone tissue engineering bracket material and preparation method thereof
  • HMBG (High Mobility Group Box) 1 modified bone tissue engineering bracket material and preparation method thereof

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Embodiment Construction

[0033] The following examples are used to illustrate the present invention, but are not intended to limit the scope of the present invention.

[0034] figure 1 A schematic diagram of HMGB1 cross-linking of the HMGB1-modified bone tissue engineering scaffold material provided by the present invention. The PLLA nanofibrous scaffold was prepared by conventional electrospinning method, and the cross-linking molecule Di-NH 2 -PEG crosslinks the signaling molecule HMGB1 to nanofibers 4 in the PLLA nanofibrous scaffold.

[0035] A PLLA solution with a mass fraction of 8-20% was prepared with hexafluoroisopropanol as a solvent, and placed in a magnetic stirrer to stir evenly. Withdraw 3-4mL of PLLA solution using a 10mL gauge medical syringe and install the syringe in the syringe pump. Cut a piece of rectangular tinfoil according to the size of the drum receiver, fix it on the surface of the drum collector, turn on the drum to make it rotate, and set the speed at 20-800r / min. Turn o...

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Abstract

The invention discloses an HMBG (High Mobility Group Box) 1 modified bone tissue engineering bracket material and a preparation method thereof. The HMBG1 modified bone tissue engineering bracket material is prepared by crosslinking a signal molecule HMGB1 to a PLLA (Poly L Lactic Acid) nanofiber bracket by means of heparin (2) and a crosslinking molecule Di-NH2-PEG (Polyethylene Glycol) by using the PLLA nanofiber bracket manufactured by an electrostatic spinning technology as a matrix. According to the invention, various functions of recruitment, infiltration and mobility of cells are realized by the signal molecule, so that the material has good biocompatibility, and can be used to compose stem cells and further can be directly applied to bone tissue repair. The material is simple and accessible in process and good in repeatability.

Description

technical field [0001] The invention relates to a bone tissue engineering scaffold material and a preparation method thereof, in particular to a HMGB1-modified nanofiber bone repair scaffold and a preparation method thereof. Background technique [0002] Tissue engineering is currently a common method for repairing bone defects, and constructing an ideal tissue engineering scaffold for bone defects is one of the key links. An ideal scaffold for bone tissue engineering should contain the following properties: good biocompatibility; provide a certain mechanical strength and integrity before the newly formed tissue can maintain its function; have osteoconduction and osteoinduction capabilities; have interconnected The three-dimensional porous microstructure and appropriate pore size enable cells / stem cells to adhere, proliferate and differentiate on the material; the material can be absorbed during tissue remodeling; bone extracellular matrix and blood vessels can grow on the m...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/22A61L27/18
Inventor 吕永钢林崇文邹杨
Owner CHONGQING UNIV
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