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A preparation method of nanofiber gel composite matrix for constructing skin tissue

A nanofiber, composite matrix technology, applied in cellulose/protein conjugated rayon, pharmaceutical formulations, non-woven fabrics, etc., can solve the problem of difficulty in preparing large-area nanofiber scaffold materials, and achieve the prevention of wound infection, Excellent biocompatibility, the effect of promoting cell recruitment

Active Publication Date: 2021-10-29
NANJING TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, based on the current fiber spinning technologies: electrospinning, microfluidic spinning and solution air-jet spinning, it seems that it is not easy to prepare large-area nanofibrous scaffold materials.

Method used

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  • A preparation method of nanofiber gel composite matrix for constructing skin tissue
  • A preparation method of nanofiber gel composite matrix for constructing skin tissue
  • A preparation method of nanofiber gel composite matrix for constructing skin tissue

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Inject 15 g of the main glue solution in the pig-source fibrin adhesive into 0.3 g of the main glue freeze-dried powder to obtain a 1.96 wt % fibrin solution. Then 0.5g PCL (weight average molecular weight 80000), 2.0g dry SF were dissolved in the solution containing 15g formic acid (the mass concentration of the formic acid solution was 85%), and it was fully stirred at room temperature for 6h to obtain a mass fraction of 14.2 wt% PCL / SF spinning solution (the mass ratio of PCL to SF is 0.25). The obtained PCL / SF spinning solution is used as the internal phase, the fibrin solution is used as the external phase, and the two solutions are used as the reservoir of the precursor, and loaded into a syringe. The two injectors were respectively connected to the two inlets of the T-shaped microfluidic chip as reactors for preparing fibrin-coated PCL / SF nanofibers. A stainless steel passivated needle (24G) was used to feed the prepared spinning solution at a fixed feed rate (0...

Embodiment 2

[0036]Inject 15 g of the main glue solution in the pig-source fibrin adhesive into 0.5 g of the main glue freeze-dried powder to obtain a 3.33 wt % fibrin solution. Then 0.9g PCL (weight-average molecular weight 80000), 2.1g dry SF are dissolved in containing 15g formic acid, make it fully stir 6h at room temperature and make mass fraction be the PCL / SF spinning solution of 16.7%wt%. 0.428, w / w). The obtained PCL / SF spinning solution is used as the internal phase, the fibrin solution is used as the external phase, and the two solutions are used as the reservoir of the precursor, and loaded into a syringe. The two injectors were respectively connected to the two inlets of the T-shaped microfluidic chip as reactors for preparing fibrin-coated PCL / SF nanofibers. A stainless steel passivated needle (24G) was used to feed the prepared spinning solution at a fixed feed rate (1.5 mL / h and 5 mL / h for the inner and outer parts, respectively). During the MBS process, the air pressure ...

Embodiment 3

[0038] Inject 15 g of the main glue solution in the pig-source fibrin adhesive into 0.75 g of the main glue freeze-dried powder to obtain a 4.76 wt % fibrin solution. Then 3.5g of PCL (weight average molecular weight 80000), 1.5g of dry SF were dissolved in a 25mL beaker containing 15g of formic acid, and it was fully stirred at room temperature for 6h to prepare a mass fraction of 25wt% PCL / SF spinning solution (2.33, w / w). The obtained PCL / SF spinning solution is used as the internal phase, the fibrin solution is used as the external phase, and the two solutions are used as the reservoir of the precursor, and loaded into a syringe. The two injectors were respectively connected to the two inlets of the T-shaped microfluidic chip as reactors for preparing fibrin-coated PCL / SF nanofibers. A stainless steel passivated needle (24G) was used to feed the prepared spinning solution at a fixed feed rate (5 mL / h and 0.1 mL / h for the inner and outer parts, respectively). During the M...

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Abstract

The invention relates to a preparation method of a nanofiber gel composite matrix for constructing skin tissue. The main gel solution in the porcine fibrin adhesive is injected into the main gel freeze-dried powder to obtain a fibrin solution; Ester and silk fibroin are added to a container containing formic acid to obtain a PCL / SF spinning solution; the PCL / SF spinning solution is used as the inner phase, and the fibrin solution is used as the outer phase to perform spinning to obtain PCL coated with fibrin / SF core-shell nanofibers composed of nanofabric; spray the freeze-dried powder catalyst solution on the obtained nanofabric to form a gel, and obtain a nanofiber gel composite matrix for constructing skin tissue. This method is easy to operate and versatile, and can achieve large-area skin regeneration in abdominal full-thickness skin defects.

Description

technical field [0001] The invention relates to a preparation method of nano-fabric, in particular to a preparation method of a nano-fiber gel composite matrix for constructing skin tissue. Background technique [0002] In nature, human beings mainly rely on the skin to avoid external intrusions. The skin, as the body's outer epithelium, maintains the body's homeostasis and repairs damage throughout life. Mimicking skin to obtain artificial materials with advanced potential is highly desirable. For this purpose, many techniques (eg, skin grafting, reprogramming of wound-resident cells) and materials (eg, porous foams, biocompatible films, biomaterials, and functional gels) have been developed. However, advances in repairing the abdomen for extensive burns or even gut exposure have been inefficient and indeed represent a major challenge in the field. In order to quickly construct patients' skin tissue for regeneration, it is necessary to develop more rapid and effective sk...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/60A61L27/22A61L27/18A61L27/50A61L27/52A61L27/56A61L27/58D04H1/4382D04H1/728D01F8/02D01F8/14
CPCA61L27/18A61L27/225A61L27/227A61L27/50A61L27/52A61L27/56A61L27/58A61L27/60A61L2300/412A61L2400/12D01F8/02D01F8/14D04H1/4382D04H1/728C08L67/04C08L89/00
Inventor 陈苏崔婷婷余加飞王格飞
Owner NANJING TECH UNIV
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