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A biomimetic multilayer collagen scaffold for cartilage repair and its preparation method

A collagen scaffold and cartilage repair technology, applied in tissue regeneration, bone implants, medical science, etc., can solve the problems of low inductivity of biomaterials, high risk of immune rejection, and high treatment costs, achieving good biocompatibility, Promotes cell recruitment and shortens treatment time

Active Publication Date: 2021-12-21
GUANGDONG GENERAL HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] Therefore, in order to be able to simultaneously solve the deficiencies in the current practice of cartilage repair, that is, low inductivity of biomaterials, high risk of immune rejection, low effectiveness and high cost of treatment, in addition to considering the selection and preparation process of biomimetic scaffold materials, it is also necessary to consider The design of its bionic space structure and the realization of its restoration function

Method used

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  • A biomimetic multilayer collagen scaffold for cartilage repair and its preparation method
  • A biomimetic multilayer collagen scaffold for cartilage repair and its preparation method
  • A biomimetic multilayer collagen scaffold for cartilage repair and its preparation method

Examples

Experimental program
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Effect test

Embodiment 1

[0080] After pulverizing the stripped tendon, add 10 times the volume of 4M guanidine hydrochloride prepared by pH=7.5, 0.05M Tris-HCl;

[0081] After suspension, pulverize the homogenate, stir at 4°C for 24 hours, centrifuge at 12000g for 20 minutes, and separate the supernatant and precipitate;

[0082] The precipitate was fully washed with Tris-HCl buffer and 0.5mM acetic acid, and then added acetic acid overnight to remove proteoglycans;

[0083] Dissolve the tendon with pepsin-containing glacial acetic acid solution, continue to process for a period of time until the mixture becomes a colorless, transparent, viscous liquid, centrifuge at 4°C for 20 minutes under 5000g centrifugal force, and collect the supernatant as the crude tendon collagen stock solution;

[0084] Add the sodium chloride solution to it, and keep stirring until white flocculent precipitates are precipitated from the solution, and continue to add the sodium chloride solution until the precipitate is no l...

Embodiment 2

[0091] After pulverizing the stripped tendon, add 10 times the volume of 4M guanidine hydrochloride prepared by pH=7.5, 0.05M Tris-HCl;

[0092] After suspension, pulverize the homogenate, stir at 4°C for 24 hours, centrifuge at 12000g for 20 minutes, and separate the supernatant and precipitate;

[0093] The precipitate was fully washed with Tris-HCl buffer and 0.5mM acetic acid, and then added acetic acid overnight to remove proteoglycans;

[0094] Dissolve the tendon with pepsin-containing glacial acetic acid solution, continue to process for a period of time until the mixture becomes a colorless, transparent, viscous liquid, centrifuge at 4°C for 20 minutes under 5000g centrifugal force, and collect the supernatant as the crude tendon collagen stock solution;

[0095] Add the sodium chloride solution to it, and keep stirring until white flocculent precipitates are precipitated from the solution, and continue to add the sodium chloride solution until the precipitate is no l...

Embodiment 3

[0102] After pulverizing the stripped tendon, add 10 times the volume of 4M guanidine hydrochloride prepared by pH=7.5, 0.05M Tris-HCl;

[0103] After suspension, pulverize the homogenate, stir at 4°C for 24 hours, centrifuge at 12000g for 20 minutes, and separate the supernatant and precipitate;

[0104] The precipitate was fully washed with Tris-HCl buffer and 0.5mM acetic acid, and then added acetic acid overnight to remove proteoglycans;

[0105] Dissolve the tendon with pepsin-containing glacial acetic acid solution, continue to process for a period of time until the mixture becomes a colorless, transparent, viscous liquid, centrifuge at 4°C for 20 minutes under 5000g centrifugal force, and collect the supernatant as the crude tendon collagen stock solution;

[0106]Add the sodium chloride solution to it, and keep stirring until white flocculent precipitates are precipitated from the solution, and continue to add the sodium chloride solution until the precipitate is no lo...

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Abstract

The invention relates to a medical material, in particular to the field of cartilage injury regeneration, and mainly discloses a bionic multi-layer collagen scaffold for cartilage repair and a preparation method thereof. The stent product of the present invention has a multi-layer three-dimensional space structure, which promotes the recruitment of cells in the body by reducing the immunogenicity of the material, and the multi-layer superposition design including a dense layer, a porous layer and a barrier layer, and realizes a biological material without exogenous cells Induced cartilage regeneration in vivo, and optimized regeneration strategies for the treatment and regeneration of cartilage injuries.

Description

technical field [0001] The invention relates to a medical material, in particular to the field of cartilage injury regeneration, and mainly discloses a bionic multi-layer collagen scaffold for cartilage repair and a preparation method thereof. Background technique [0002] Osteochondral defects in joints caused by trauma or bone disease are common in clinical practice and seriously affect the quality of life of patients. It has become one of the main causes of physical disability. Knee disease can occur in people of all ages. Cartilage defects in the knee can result from trauma, sprains, overuse of the knee, muscle weakness, or general wear and tear. Due to the special tissue structure and biological characteristics of articular cartilage, although many methods are known to treat articular cartilage defects, including but not limited to: subchondral bone drilling, electrical stimulation, laser, drug and cell injection, gene therapy, etc., However, none of the above methods...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/40A61L27/24A61L27/56A61L27/12A61L27/50A61F2/28A61F2/30
CPCA61L27/24A61L27/56A61L27/12A61L27/50A61F2/28A61F2/30756A61F2/30942A61F2/2846A61L2430/06A61F2002/2835A61F2310/00293
Inventor 郑秋坚邓展涛马元琛李梦远郑铭豪
Owner GUANGDONG GENERAL HOSPITAL
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