Preparation method and application of targeted chondrocyte exosome

A technology of chondrocytes and exosomes, which is applied in the direction of biochemical equipment and methods, animal cells, vertebrate cells, etc., can solve the problem of poor delay of osteoarticular cartilage damage, poor improvement of cartilage matrix destruction, and cartilage matrix decomposition. Fast metabolism and other problems, to achieve the effect of delaying the cartilage damage process, improving the cartilage matrix damage, and controlling the damage of articular cartilage

Inactive Publication Date: 2021-06-25
THE SECOND PEOPLES HOSPITAL OF SHENZHEN
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Problems solved by technology

[0003] In the prior art, targeting chondrocyte exosomes has poor effect on improving cartilage matrix damage, and has poor effect on delaying the process

Method used

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Embodiment Construction

[0023] The technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention. Apparently, the described embodiments are only some of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

[0024] The present invention provides a technical solution: a method for preparing exosomes targeting chondrocytes, comprising the following steps:

[0025] S1, preparing exosome vectors, first constructing the chondrocyte-targeting peptide-modified lamp2b recombinant plasmid, and then extracting chondrocyte-affinity targeting exosomes;

[0026] S2, preparing cartilage-targeted exosomes, transfecting mouse dendritic cells with the CAP-lamp2b recombinant plasmid genetically mo...

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Abstract

The invention provides a preparation method of a targeting chondrocyte exosome. The preparation method comprises the following steps of preparing an exosome carrier, preparing a targeting cartilage exosome, and preparing miRNA-140 wrapped by the targeting cartilage exosome. The preparation method has the beneficial effects that the miRNA medicine is delivered with treatment potential, high efficiency and tissue specificity for treating osteoarthritis, the application of the miRNA-140 wrapped by the targeting exosome in the medicine for treating osteoarthritis is mainly utilized, in an osteoarthritis animal model of a rat, the expression of MMP-13 can be inhibited by injecting the miRNA-140 into a knee joint cavity, the damage of cartilage matrix can be obviously improved, the cartilage damage process of an osteoarthritis animal model is delayed, and the cartilage protection effect is achieved, and therefore, the exosome is used for transporting miRNA-140 in a targeted manner, degradation of cartilage tissues can be slowed down, and the effect of inhibiting catabolism of cartilage matrix is achieved, so that the damage of articular cartilage is controlled, and cartilage repair is promoted.

Description

technical field [0001] The invention relates to the technical field of preparation of chondrocyte exosomes, in particular to a preparation method and application of targeted chondrocyte exosomes. Background technique [0002] Targeted delivery of drug molecules to specific types of cells is a major challenge in precision medicine. Natural exosomes have biofunctionalized nanovesicles that can display a variety of targeting proteins through genetic engineering. Or the ligand of the polypeptide and directly package the drug to exercise the ability of drug targeted delivery; [0003] In the prior art, targeting chondrocyte exosomes has poor effect on improving cartilage matrix damage, and has poor effect on delaying the process of osteoarticular cartilage damage, resulting in faster catabolism of cartilage matrix, which is not conducive to controlling articular cartilage damage. Contents of the invention [0004] The purpose of the present invention is to provide a preparatio...

Claims

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Application Information

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IPC IPC(8): A61K9/127A61K31/7088A61K47/46A61P19/02A61P29/00C12N15/85C12N5/10
CPCA61K9/1273A61K31/7088A61K47/46A61P19/02A61P29/00C07K14/70596C12N5/0625C12N15/85C12N2510/00C12N2800/107
Inventor 徐晓段莉梁宇杰徐丽梅
Owner THE SECOND PEOPLES HOSPITAL OF SHENZHEN
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