14 results about "Continuous flow centrifugation" patented technology

The invention discloses a corn peptide for dispelling effects of alcohol and protecting liver as well as a preparation method and application thereof, and belongs to the technical field of biological peptide extraction. The preparation method comprises the following steps: crushing and sieving corn, dissolving the corn in deionized water, adjusting the pH value to be alkaline, and performing ultrasonic-assisted extraction to obtain corn homogenate; centrifuging, taking supernate, adjusting the pH to be neutral, centrifuging, adding an ethanol solution with the mass fraction of 10%, standing, carrying out continuous flow centrifugation, suspending precipitates in distilled water, dialyzing in a dialysis bag, and freeze-drying to obtain crude corn protein; the preparation method comprises the following steps: preparing an aqueous solution, carrying out variable-temperature treatment, adjusting the pH value to be alkaline, adding trypsin and alkaline protease, carrying out enzymolysis, repeating the enzymolysis twice, and after the enzymolysis is finished, carrying out heating enzyme deactivation, centrifugation, sediment discarding, ultrafiltration, centrifugation, supernatant taking and spray drying to obtain the corn peptide. The corn peptide can remarkably shorten the drunkenness time and sobering time of mice and reduce the drunkenness duration, and has good effects of dispelling the effects of alcohol and protecting the liver.

The invention discloses an abelmoschus manihot exosome extraction process, and relates to the field of extraction and purification in biotechnology, and the abelmoschus manihot exosome extraction process comprises the following steps: (1) providing a fresh flower material of abelmoschus manihot, and carrying out mechanical cleaning and surface impurity removal; and (2) homogenizing the cleaned fresh flowers of the abelmoschus manihot for 5-30 minutes by a high-shear homogenizer under the condition that the rotating speed is 8000-15000rpm to obtain homogenate. The method is specially designed for the physiological characteristics of the fresh flowers of the abelmoschus manihot, and through the core principle of'rapid treatment and low-temperature operation ', the activity loss of the exosomes in the extraction process is reduced to the greatest extent, so that the yield of the exosomes is increased, and the yield of the abelmoschus manihot is increased. The method ensures the integrity of the natural structure and function, creatively adopts a process route of combining continuous flow centrifugation and multistage tangential flow ultrafiltration, realizes continuous and efficient separation and purification of exosomes from a large number of plant raw materials, solves the industrial bottleneck that a traditional ultracentrifugation method cannot realize large-scale production, and is suitable for industrial production. And the treatment capacity can easily reach 5-15 tons of fresh flowers per day.

The invention belongs to the technical field of polypeptide extraction, and particularly discloses an extraction method of camellia seed meal polypeptide. The method comprises the following steps: crushing and sieving camellia oleifera seed meal, mixing with pure water, adjusting the pH value to be alkaline, carrying out microwave-assisted extraction, and carrying out continuous flow centrifugation to obtain supernate; adjusting the pH value to be acidic, standing, carrying out continuous flow centrifugation, collecting precipitate, washing, and carrying out spray drying to obtain camellia seed meal crude protein; the preparation method comprises the following steps: preparing an aqueous solution, heating for denaturation treatment, cooling, adjusting the pH value to acidity, standing, adjusting the pH value to alkalescence, synchronously adding neutral protease, trypsin and alkaline protease, carrying out enzymolysis, enzyme deactivation, centrifugation, sediment discarding, ultrafiltration membrane filtration, tangential flow filtration desalination, filtration, supernatant taking, continuous flow centrifugation, supernatant taking and spray drying, thereby obtaining the camellia seed meal polypeptide. The invention discloses an extraction method of camellia seed meal polypeptide, which can improve the yield of polypeptide, and the polypeptide extracted by the method has high activity and is easily absorbed by organisms.

The invention relates to a centrifuge for continuous flow centrifugation, wherein the centrifuge (1) comprises a drum (3), a rotor (4) and a motor (5) for driving the drum (3) and the rotor (4), wherein the centrifuge (1) comprises a drive bearing arrangement (7) with at least two drive roller bearings (8) with an inner race (9), an outer race (10) and a lubricant, wherein one of the drive roller bearings (8) comprises a sealing (11), wherein the drive roller bearings (8) rotate around the common rotation axis (A), wherein the drive bearing arrangement (7) comprises a bearing housing (12) with an outer wall (13). It is proposed that the outer wall (13) comprises a ridge (14) connected to the outer wall (13) by a connection providing a sealing (11) function and extending from the outer wall (13) towards the inner races (9) along the sealing (11).

A control system for a centrifuge is disclosed, that comprises: a controller that receives a first pressure signal indicative of a first pressure of a product flowing into or out of the centrifuge, and a second pressure signal indicative of a second pressure of an operating fluid flowing into or out of the centrifuge. The controller is configured to: determine a first pressure difference between the first pressure and the second pressure; an in response to the first pressure difference dropping below a first predetermined offset, output a first control signal that causes a back pressure of the product flowing out of the centrifuge to increase.

The invention relates to a centrifuge for continuous flow centrifugation with a drum (3), a rotor (4) and a motor (5) for driving the drum (3) and the rotor (4), wherein the drum (3), driven by the motor (5), rotates around a common rotation axis (A) with a rotational frequency during use of the centrifuge (1), wherein the rotor (4) is coupled to the drum (3), wherein the rotor (4) rotates around the common rotation axis (A) with the double of the rotational frequency, wherein the centrifuge (1) comprises at least one main bearing (7) between the rotor (4) and the drum (3) via which the rotor (4) is mounted onto the drum (3). It is proposed that the rotor (4) comprises at least one cooling fin (10) which moves air in a direction radially outwards from the common rotation axis (A) when the rotor (4) rotates.

The invention relates to a centrifuge for continuous flow centrifugation with drum (3), a rotor (4) and a motor (5), wherein the drum (3), driven by the motor (5), rotates around a common rotation axis (A) with a rotational frequency during use of the centrifuge (1), wherein the rotor (4) is coupled to the drum (3), wherein the centrifuge (1) comprises a drive bearing arrangement (7) with at least two drive ball bearings (8), wherein the drive ball bearings (8) each comprise an inner race (9), an outer race (10) and a bearing axis (B), wherein the drive ball bearings (8) rotate around the common rotation axis (A). It is proposed that the drive ball bearings (8) are angular drive ball bearings (8).

A control system for a centrifuge is disclosed that includes a controller that receives a first pressure signal indicative of a first pressure of a product flowing into or out of the centrifuge and a second pressure signal indicative of a second pressure of a working fluid flowing into or out of the centrifuge. The controller is configured to determine a first pressure differential between the first and second pressures and, in response to the first pressure differential falling below a first predetermined offset, output a first control signal that increases a backpressure of the product flowing out of the centrifuge.

The present invention relates to a squid cartilage hydrolyzate, a large-scale preparation method and its application. Using squid cartilage discarded from aquatic product processing plants as raw material, first use acid solution to remove calcium salts, then use high-temperature acid hydrolysis reaction to quickly hydrolyze the squid cartilage into a mixture of oligosaccharides and oligopeptides, and then use Maillard reaction and ethanol to remove fishy smell to improve the flavor, and further use continuous flow centrifugation synergistic membrane separation technology to separate and purify the crude extract of squid cartilage hydrolyzate to obtain a squid cartilage hydrolyzate solution with a content of ≥95%. Compared with the enzymatic hydrolysis method, the method of the present invention has a higher yield and consumes less time. At the same time, the squid cartilage hydrolyzate produced by the present invention has better antibacterial activity than potassium sorbate and sodium benzoate; has better water absorption performance than glycerol; has better antioxidant activity than butylated hydroxyanisole (BHA) and vitamin E, and its flavor is better.

This invention discloses a centrifugal recovery device, relating to the field of biological cell processing technology. The centrifugal recovery device includes a centrifuge container and a paddle assembly. The centrifuge container includes a centrifuge chamber, with a collection area at the top for easy discharge of waste liquid. The paddle assembly is located within the centrifuge chamber and includes paddles and a paddle shaft. The centrifuge container and the paddle shaft share the same axis of rotation. A first flow channel and a second flow channel are formed between the paddles and the centrifuge container. The paddle shaft has an axially arranged inlet channel and a recovery channel. The inlet channel communicates with the centrifuge chamber. The first flow channel is arranged on the inner circumference of the centrifuge container, with one side communicating with the inner cavity of the centrifuge chamber. The second flow channel communicates with both the first flow channel and the recovery channel. This invention provides a continuous flow centrifugal recovery device for cell processing, which features a long cell fluid movement path, improving centrifugation efficiency and effectiveness, resulting in more thorough separation of cells from the supernatant, and increasing cell yield and viability.

The utility model relates to a kind of continuous flow centrifuge rotor and its baffle structure, by baffle structure, rotor body inner cavity is separated into multiple independent areas, multiple through holes are distributed to each independent area by the liquid to be separated, and the liquid level of each independent area is kept consistent when gap is maintained in liquid inflow. Baffle structure separates rotor body inner cavity, reduces the generation of vortex phenomenon, through hole ensures that liquid is uniformly distributed to each independent area, and gap keeps liquid level consistent during liquid flow, thereby effectively inhibiting vortex phenomenon, ensuring that particles are uniformly settled, and improving separation efficiency.

The application discloses an extracellular vesicle extraction system, application and method for extracting extracellular vesicles, and belongs to the technical field of extracellular vesicle separation. The extracellular vesicle extraction system comprises a raw material storage tank, a deep filtration device, a continuous flow centrifuge, a pretreatment buffer tank, a first filtration device, a concentrated liquid tank, a second filtration device, a product collection tank and a waste liquid tank. The extracellular vesicle extraction system is used for extracting extracellular vesicles, and based on the ultrafiltration and microfiltration technology, the extracellular vesicles in the crushed liquid of Chinese herbal medicines or other plants are separated. Compared with the existing differential centrifugation, density gradient centrifugation, ultrafiltration, immunocapture and other technologies, the process is more suitable for large-batch, standardized and high-efficiency extraction, the product yield is high, the speed is fast, the service life of the filter membrane is greatly prolonged, the process cost is reduced, and the process has great commercial value.

The present invention relates to a large-scale rapid preparation method for a rice water hydrolysate suitable for cosmetics. The rice water collected after rice washing is used as raw material. A continuous flow centrifugal-coordinated membrane separation technique is first used for solid-liquid separation, desalination, and impurity removal, followed by concentration to obtain a rice water concentrate. The solid obtained by centrifugation is then mixed with the rice water concentrate, and then subjected to a high-temperature rapid acid hydrolysis reaction to obtain a rice water hydrolysate solution having a content of ≥95%. Finally, the rice water hydrolysate is rapidly dried using a spray drying technique to form a rice water hydrolysate powder. The method of the present invention is simple and feasible overall, has convenient process operation, is low in cost, and is highly efficient, making it more suitable for large-scale production. The prepared rice water hydrolysate has a low molecular weight (substantially less than 5000 Da), a high molecular weight distribution concentration, and a high content of B vitamins. Furthermore, it exhibits excellent moisture absorption and moisture retention properties and antioxidant activity, making it particularly suitable for use in various cosmetics.