The invention discloses an immune cell culture method. The method comprises the following steps: S1, preparation work, S2, sample dilution, S3, gradient centrifugation, S4, buffy coat collection, S5, washing, S6, washing, S7, inoculation, S8, addition of feeder cells, S9, culture start, S10, observation and recording, and S11, cryopreservation. According to the immune cell culture method, the conditions that manual operation of cell culture medium blowing is time-consuming and laborious and the blowing effect is poor are avoided, the blowing efficiency is improved, centrifugal treatment of the cell culture medium can be achieved through first centrifugal mechanisms and second centrifugal mechanisms, by arranging four first centrifugal mechanisms and four second centrifugal mechanisms outside a blowing mixing mechanism at one time, multi-station operation is achieved, a large number of immune cells can be cultured at one time, and the device is high in efficiency and is convenient in popularization.