Patch for promoting wound healing and preparation method thereof

A wound healing and patch technology, applied in biochemical equipment and methods, cell dissociation methods, sheet delivery, etc., can solve the problems of unavailable spot supply, harsh storage conditions of cells or factors, etc., to promote wound healing, The effect of low instrument requirements and convenient use

Pending Publication Date: 2022-01-04
陕西医赛尔生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The present invention aims at the technical problem that the active ingredients for promoting wound healing in the prior art are mostly injected or applied in the form of living cells. Due to the harsh storage conditions of the cells or factors, the technical problem that spot supply cannot be realized at room temperature, and there are no literature and patent reports to promote wound healing. Application of healing active ingredients in patches

Method used

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  • Patch for promoting wound healing and preparation method thereof
  • Patch for promoting wound healing and preparation method thereof
  • Patch for promoting wound healing and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Embodiment 1: the preparation method of patch for promoting wound healing

[0022] The present invention specifically provides a preparation for promoting wound healing patches, comprising the following steps:

[0023] (1) Use mTeSR1 human ESC / iPSC medium without xenogeneic animal-derived components to culture umbilical cord mesenchymal stem cells in a culture mode without feeder cells. The culture conditions are 37°C, 5%-10% O2 and 10%-15 %CO2, change the medium every day, and culture for 15 days.

[0024] (2) During the culture process, add EDTA to the culture dish, incubate at 37°C for 1-10 minutes to obtain the digestion solution, absorb the above digestion solution, blow the cells down gently, and transfer them to the ultra-low adsorption culture containing mTeSR1 human ESC / iPSC medium Suspension culture was carried out in a dish to form embryoid bodies.

[0025] (3) During the continuous suspension culture of embryoid bodies, the induction medium combination TeS...

Embodiment 2

[0029] Embodiment 2: the preparation method of patch for promoting wound healing

[0030] On the basis of the preparation method in Example 1, a preparation method of a patch with active ingredients for promoting wound healing is provided, and 5% O2 in mTeSR1 human ESC / iPSC medium is provided. 2 and 10% CO 2 During the culture, add EDTA to the culture dish, incubate at 37°C for 1 min, and add the induction medium combination TeSR during the suspension culture of the embryoid bodies TM -E8 TM Human ESC / iPSC medium and AR-A014418 were used to induce differentiation at a concentration of 1 μM, fetal bovine serum was added, and the final compound concentration was 1%.

Embodiment 3

[0031] Embodiment 3: the preparation method of patch for promoting wound healing

[0032] On the basis of the preparation method in Example 1, a preparation method of a patch with active ingredients for promoting wound healing is provided. In mTeSR1 human ESC / iPSC medium, 7% O 2 and 12% CO 2 During the culture, add EDTA to the culture dish, incubate at 37°C for 4min, and add the induction medium combination TeSR during the suspension culture of the embryoid body TM -E8 TM Human ESC / iPSC medium and AR-A014418 were used to induce differentiation at a concentration of 5 μM, fetal bovine serum was added, and the final compound concentration was 5%.

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PUM

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Abstract

The invention discloses a preparation method of a patch for promoting wound healing. According to the invention, an ultralow temperature cell lysis technology is combined with continuous flow centrifugation to separate out active components having a promoting effect on wound healing in MSCs, the active components for promoting wound healing are finally obtained by optimizing process parameters of the active components for wound healing, the active components are bound on a carrier patch for freeze-drying, the patch containing stem cell active ingredients and capable of promoting wound healing can be provided, the preparation method is simple, the requirement for instruments is not high, the patch is suitable for mass production and convenient to use, umbilical cord mesenchymal stem cells serve as the active ingredients of the patch, the use allergy rate is low, and the application range is wide.

Description

technical field [0001] The invention mainly relates to the technical field of external medicine, in particular, the invention relates to a patch for promoting wound healing and a preparation method thereof. [0002] technical background [0003] Wound repair has always been a hot topic in the medical field. In my country, there are tens of millions of patients with wounds caused by diabetes and vascular sclerosis every year. As the largest organ of the human body, the skin is the body's first line of defense against external aggression, and it is also the most prone to ulceration defects or functional loss . Skin wound repair is a complex and orderly process that requires the coordinated participation of various cells and active molecules. Although the current full-thickness skin transplantation and tissue engineering skin technology are becoming more and more perfect, there are still a series of problems to be solved, such as: immune rejection after allogeneic skin transpla...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/28A61K9/70A61P17/02C12N5/0775
CPCA61K35/28A61K9/7023A61P17/02C12N5/0668C12N2509/00
Inventor 杨劲鹏张小波
Owner 陕西医赛尔生物科技有限公司
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