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Large-scale preparation method of sulfated kelp oligosaccharide suitable for cosmetics

A kelp oligosaccharide and sulfation technology, which is applied in the field of chemical extraction technology and cosmetics, can solve the problems of undiscovered sulfated kelp oligosaccharide preparation technology and process, which is not conducive to the absorption of kelp oligosaccharide, molecular weight distribution dispersion, etc., to avoid time Low cost and expensive consumables, good absorption, high concentration of molecular weight distribution

Active Publication Date: 2021-03-19
THIRD INST OF OCEANOGRAPHY MINIST OF NATURAL RESOURCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The laminar oligosaccharide prepared by the above method has a wide distribution of polymerization degree and a relatively dispersed molecular weight distribution, and the unique natural sulfate radical of laminarin is lost, which is not conducive to the faster absorption of laminar oligosaccharide by the human body
At present, no patent literature reports on the preparation technology and process of sulfated laminar oligosaccharides that are particularly suitable for cosmetics have been found.

Method used

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  • Large-scale preparation method of sulfated kelp oligosaccharide suitable for cosmetics
  • Large-scale preparation method of sulfated kelp oligosaccharide suitable for cosmetics
  • Large-scale preparation method of sulfated kelp oligosaccharide suitable for cosmetics

Examples

Experimental program
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Effect test

Embodiment 1

[0058] A large-scale preparation method of sulfated laminaria oligosaccharides suitable for cosmetics, the steps are as follows:

[0059] (1) Pretreatment

[0060] Put the kelp raw material into the reaction kettle, add a sodium bicarbonate solution 10 times the weight of the kelp, stir for 30 minutes, rinse with clean water, wherein the mass volume concentration of the sodium bicarbonate solution is 1%; put the washed kelp into the reaction kettle Add deionized water 10 times the weight of kelp, homogenate at high speed for 30 minutes, the homogenate speed is 16000 rpm, after the homogenization is completed, the temperature is raised to 55°C, the pH is adjusted to 6.5, and papain with 0.5% of the weight of kelp is added , reacted for 60 minutes; after enzymolysis, use a continuous flow centrifuge to centrifuge at a high speed at 14000rpm, collect the kelp residue after centrifugation and put it into the reactor again, add deionized water 10 times the weight of kelp, stir for ...

Embodiment 2

[0068] A large-scale preparation method of sulfated laminaria oligosaccharides suitable for cosmetics, the steps are as follows:

[0069] (1) Pretreatment

[0070] Put the kelp raw material into the reaction kettle, add potassium bicarbonate solution 5 times the weight of kelp, stir for 45 minutes, rinse with clean water, wherein the mass volume concentration of the potassium bicarbonate solution is 1%; put the washed kelp into the reaction kettle , add deionized water 5 times the weight of kelp, homogenate at a high speed for 60 minutes, the homogenate speed is 12000 rpm, after the homogenization is completed, the temperature is raised to 60 ° C, the pH is adjusted to 7.0, and papain with 0.5% of the weight of kelp is added React with 0.5% neutral protease for 45 minutes; after enzymolysis, use a continuous flow centrifuge to centrifuge at a high speed at 14,000rpm, collect the kelp residue after centrifugation and put it into the reactor again, add deionized water 15 times t...

Embodiment 3

[0078] A large-scale preparation method of sulfated laminaria oligosaccharides suitable for cosmetics, the steps are as follows:

[0079] (1) Pretreatment

[0080] Put the kelp raw material into the reaction kettle, add the sodium hydroxide solution 10 times the weight of the kelp, stir for 15 minutes, rinse with clean water, wherein the mass volume concentration of the sodium hydroxide solution is 4%; put the washed kelp into the reaction kettle , add deionized water 3 times the weight of kelp, homogenize at high speed for 60 minutes, the homogenization speed is 10000 rpm, after the homogenization is completed, the temperature is raised to 53 ° C, the pH is adjusted to 6.5, and papain with 1.0% of the weight of kelp is added React with 1.0% flavor protease for 30 minutes; after enzymolysis, use a continuous flow centrifuge to centrifuge at a high speed at 14,000rpm, collect the kelp residue after centrifugation and put it into the reactor again, add 15 times the weight of kel...

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Abstract

The invention relates to a large-scale preparation method of sulfated kelp oligosaccharide suitable for cosmetics. The method comprises the following steps of: by taking kelp as a raw material, performing pretreatment, namely quickly rinsing and degreasing by adopting an alkali solution, performing high-speed homogenate grinding and protease enzymolysis to quickly remove all crude proteins and partial mannitol and ash, performing high-temperature oxidative hydrolysis, quickly preparing the pretreated kelp residues into a sulfated kelp oligosaccharide primary extract, separating and purifying the sulfated kelp oligosaccharide extract at normal temperature by utilizing a continuous flow centrifugation and membrane separation technology, thereby obtaining a sulfated kelp oligosaccharide solution, and finally, quickly drying by utilizing a spray drying technology, thereby forming the sulfated kelp oligosaccharide powder. The method disclosed by the invention is simple and feasible in overall process, high in efficiency, energy-saving and suitable for large-scale production; the prepared sulfated kelp oligosaccharide is high in molecular weight concentration ratio and even can be comparable with a dextran standard product; and moreover, the prepared sulfated kelp oligosaccharide with the molecular weight of less than 1000 is particularly suitable for being used in various cosmetics.

Description

technical field [0001] The invention relates to a large-scale preparation method of sulfated laminar oligosaccharides suitable for cosmetics, and belongs to the fields of chemical extraction technology and cosmetics. Background technique [0002] As an intercellular polysaccharide inherent in brown algae such as kelp, laminarin exists in the cell wall matrix of brown algae and is a unique active substance of brown algae. Laminaria polysaccharide is a kind of multi-component mixture. The composition and activity of polysaccharides obtained from kelp by different extraction methods are quite different. In addition, laminar polysaccharide has defects such as complex molecular structure, poor absorption, and difficult quality control, which need to be solved urgently. Laminar oligosaccharides are hydrolyzed products of laminar polysaccharides. There are many reports that low molecular weight laminar oligosaccharides also have biological activities such as anti-tumor, anti-oxidat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H3/06C07H1/08A61K8/60A61K8/9711A61Q19/00A61Q19/08
CPCC07H3/06C07H1/08A61K8/60A61K8/9711A61Q19/00A61Q19/08A61K2800/805Y02P20/10
Inventor 陈思谨洪专张怡评
Owner THIRD INST OF OCEANOGRAPHY MINIST OF NATURAL RESOURCES
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