Large-scale preparation method of protamine peptide

Abstract

The invention relates to a large-scale preparation method of protamine peptide. The method comprises the following steps: by taking mature testis tissues of aquatic organisms as raw materials, carrying out pretreatment by adopting a weak base salt solution for cleaning, then hydrolyzing testis nucleoprotein by adopting high-temperature sulfuric acid, adding reducing sugar under a high-temperature condition to carry out Maillard reaction, and then carrying out adsorption deodorization, continuous flow centrifugal filtration and multi-stage membrane separation to obtain a nucleotide-free protamine peptide concentrated solution with the content larger than or equal to 95%. Although a chemical extraction method is adopted, compared with the protamine peptide prepared by a current hot enzymolysis process, the protamine peptide prepared by the method disclosed by the invention is higher in yield and less in time consumption. Meanwhile, the production cycle of large-scale preparation of the protamine peptide can be basically controlled to be completed within 24 hours, so that the production cycle is shorter and more compact, the whole process is simpler and more efficient, and is more suitable for large-scale production. Compared with a protamine crude extract, the protamine peptide produced by the invention has better flavor in the aspects of smell and taste, and is easier to accept and eat by vast consumers.

Description

Technical field [0001] The present invention relates to a method of preparation of a fishqueous protein peptide, which belongs to the chemical extraction technology and the field of food. Background technique [0002] The nest of the aquatic animals is commonly known as fish white, also known as fish, Nucleovrotein, as well as multiple enzymes and mineral elements. The main component of the nuclear protein is about 2 / 3 of the deoxyribonucleic acid (DNA) and about 1 / 3 of the fish essence. Among them, protamine is a basic protein with a small molecular weight (from several thousand to 10,000 Dalton), which is generally consisting of 30 to 50 amino acids and is rich in arginine. Fish protein has broad-spectrum inhibitory, lowering blood pressure, inhibits tumor growth and promoting digestion, fish precision protein pure products can restore heparin anticoagulation effects, the only one of the US Food and Drug Administration (FDA) approved Anti-citrugation drugs against heparin in vi...

AI Technical Summary

Problems solved by technology

The protamine obtained by the conventional method of extracting protamine based on the current literature reports (normal temperature acid extraction+salting out+alcohol precipitation) is actually the crude extract of protamine, which still has an annoying and The fishy smell that most people cannot accept

And though the fishy smell of the pure product of protamine obtained by further column chromatography (gel chromatography or ion exchange chromatography) purification has obvious improvement, the whole purification step is complex, time-consuming and costly, and cannot be realized in the industry. scaling up and mass production

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