Large-scale preparation method of protamine peptide

A protamine and nucleoprotein technology, applied in the field of large-scale preparation of protamine peptides, can solve the problems of complicated and time-consuming purification steps, high cost, inability to realize industrialized amplification and large-scale production, etc., and achieves good flavor and time consumption. Less and more productive effects

Pending Publication Date: 2022-01-14
THIRD INST OF OCEANOGRAPHY MINIST OF NATURAL RESOURCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The protamine obtained by the conventional method of extracting protamine based on the current literature reports (normal temperature acid extraction+salting out+alcohol precipitation) is actually the crude extract of protamine, which still has an annoying and The fishy smell that most people cannot accept
And though

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  • Large-scale preparation method of protamine peptide
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  • Large-scale preparation method of protamine peptide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] A method of preparation of a fish essence peptide, steps are as follows:

[0049] (1) Raw material pretreatment

[0050] In the reactor in the reactor, the sodium bicarbonate is added 4 times the sodium hydrogencarbonate mixture with sodium chloride, stirred for 2 hours, washed with water, where the mass concentration of sodium bicarbonate is 3%, salt solution The mass volume concentration is 5%;

[0051] (2) High temperature sulfuric acid hydrolyzed nest nuclear protein

[0052] The nest of the pretreated and washed, was placed in the reaction kettle, adding deionized water having a weight nest weight, adding concentrated sulfuric acid to adjusting pH of 2, and the reaction temperature is 95 ° C, stirred for 4 hours;

[0053] (3) Merad reaction

[0054] Under high temperature conditions, the calcium hydroxide regulation pH is 6, and then the sodium hydroxide is added to 7.5; adding a glucose with a sodium flexible nest weight, the reaction temperature is 85 ° C, stirred fo...

Embodiment 2

[0062] A method of preparation of a fish essence peptide, steps are as follows:

[0063] (1) Raw material pretreatment

[0064] In the reactor, the squid is added to the reaction kettle, and the weight of the squid is 2 times the weight of sodium hydrogen carbonate and the sodium chloride mixture, stirred for 3 hours, washed with water, where the mass concentration of the potassium carbonate solution is 4%, salt solution The mass volume concentration is 4%;

[0065] (2) High temperature sulfuric acid hydrolyzed nest nuclear protein

[0066] The essence of the pretreated and washed in the reactor is placed in the reaction kettle, and a deionized water having a weight nest weight is added, and the concentrated sulfuric acid regulates the pH of 2.5, and the reaction temperature is 100 ° C, stirred for 3 hours;

[0067] (3) Merad reaction

[0068] Under high temperature conditions, the calcium hydroxide regulation pH was added to 5.5, and then the potassium hydroxide was adjusted pH o...

Embodiment 3

[0076] A method of preparation of a fish essence peptide, steps are as follows:

[0077] (1) Raw material pretreatment

[0078] In the reactor in the reactor, the squid was added to the reaction kettle, and the weight of the squid was added to the sodium bicarbonate mixture with the potassium chloride, stirred for 1 hour, washed with water, where the mass concentration of sodium bicarbonate solution was 2%, the quality of the salt solution The volume concentration is 3%;

[0079] (2) High temperature sulfuric acid hydrolyzed nest nuclear protein

[0080] The nest of the squid passes through the pretreated and washed, and a deionized water of 0.5 times the weight of the fine nest is added, and the concentrated sulfuric acid is adjusted to be 3, and the reaction temperature is 90 ° C, stirred for 4 hours;

[0081] (3) Merad reaction

[0082] Under high temperature conditions, the calcium hydroxide regulation pH was added to 6.5, and then the sodium hydroxide was adjusted to be 8.5; ...

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Abstract

The invention relates to a large-scale preparation method of protamine peptide. The method comprises the following steps: by taking mature testis tissues of aquatic organisms as raw materials, carrying out pretreatment by adopting a weak base salt solution for cleaning, then hydrolyzing testis nucleoprotein by adopting high-temperature sulfuric acid, adding reducing sugar under a high-temperature condition to carry out Maillard reaction, and then carrying out adsorption deodorization, continuous flow centrifugal filtration and multi-stage membrane separation to obtain a nucleotide-free protamine peptide concentrated solution with the content larger than or equal to 95%. Although a chemical extraction method is adopted, compared with the protamine peptide prepared by a current hot enzymolysis process, the protamine peptide prepared by the method disclosed by the invention is higher in yield and less in time consumption. Meanwhile, the production cycle of large-scale preparation of the protamine peptide can be basically controlled to be completed within 24 hours, so that the production cycle is shorter and more compact, the whole process is simpler and more efficient, and is more suitable for large-scale production. Compared with a protamine crude extract, the protamine peptide produced by the invention has better flavor in the aspects of smell and taste, and is easier to accept and eat by vast consumers.

Description

Technical field [0001] The present invention relates to a method of preparation of a fishqueous protein peptide, which belongs to the chemical extraction technology and the field of food. Background technique [0002] The nest of the aquatic animals is commonly known as fish white, also known as fish, Nucleovrotein, as well as multiple enzymes and mineral elements. The main component of the nuclear protein is about 2 / 3 of the deoxyribonucleic acid (DNA) and about 1 / 3 of the fish essence. Among them, protamine is a basic protein with a small molecular weight (from several thousand to 10,000 Dalton), which is generally consisting of 30 to 50 amino acids and is rich in arginine. Fish protein has broad-spectrum inhibitory, lowering blood pressure, inhibits tumor growth and promoting digestion, fish precision protein pure products can restore heparin anticoagulation effects, the only one of the US Food and Drug Administration (FDA) approved Anti-citrugation drugs against heparin in vi...

Claims

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Application Information

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IPC IPC(8): A23J3/04A23J3/32
CPCA23J3/04A23J3/32Y02P20/10
Inventor 陈思谨洪专张怡评谢全灵陈晖陈伟珠
Owner THIRD INST OF OCEANOGRAPHY MINIST OF NATURAL RESOURCES
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